FB2024_03 , released June 25, 2024
Allele: Dmel\Cdk2hs.PS
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General Information
Symbol
Dmel\Cdk2hs.PS
Species
D. melanogaster
Name
heat shock construct of Stern
FlyBase ID
FBal0030734
Feature type
allele
Associated gene
Dmel\Cdk2
Associated Insertion(s)
Carried in Construct
P{hs-Cdk2.BS}
Key Links
Transgenic product class
cDNA
(Stern et al., 1993)
wild_type
(Stern et al., 1993)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Stern et al., 1993)
wild_type
(Stern et al., 1993)
Mutagen
in vitro construct
(Stern et al., 1993)
Progenitor genotype
Carried in construct
P{hs-Cdk2.BS}
Cytology
Description

An Hsp70 promoter drives expression of a Cdk2 cDNA.

(Stern et al., 1993)
Allele components
Component
Use(s)
Regulatory region(s)
Hsp70
Encoded product / tool
Cdk2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Heat shock does not rescue deficit of cdc2 product.

      (Stern et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      Cdk2hs.PS is a suppressor of eye phenotype of Zzzz\aptamerhs.Cdk2.pep4

      (Kolonin and Finley, 1998)
      NOT Suppressor of
      Statement
      Reference

      Cdk2hs.PS is a non-suppressor of phenotype of Eip63EE1/Eip63EGN50

      (Stowers et al., 2000)
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference

      Suppresses the rough eye phenotype caused by overexpression of Zzzz\aptamerhs.cdc2c.pep4.T:Ecol\trxA. Has little effect on the rough eye phenotype caused by overexpression of Zzzz\aptamerhs.cdc2.pep8.T:Ecol\trxA.

      (Kolonin and Finley, 1998)
      Complementation and Rescue Data
      Rescues

      Cdk2hs.PS rescues Cdk22/Cdk21

      (Lane et al., 2000)

      Cdk2hs.PS rescues Cdk23/Cdk22

      (Lane et al., 2000)
      Comments

      Periodic expression of cdc2chs.PS (using heat shock) in cdc2c2/cdc2c3 animals allows them to develop into morphologically normal and fertile adults. Some animals are still observed to develop into adults even if the onset of periodic expression of cdc2chs.PS is delayed until 116 hours after egg deposition. cdc2c2/cdc2c3 or cdc2c1/cdc2c2 females that have been rescued by periodic expression of cdc2chs.PS readily lay eggs as long as they are subjected to periodic heat shocks (to induce expression of cdc2chs.PS). However, after termination of periodic heat shocks, egg deposition decreases rapidly and stops completely within 2-3 days. This arrest of egg deposition can be readily reversed within 7 days after resumption of periodic heat shocks. Eggs collected from females 1 day after termination of periodic heat shocks often show abnormal spatial distribution of nuclei and the chromatin often appears abnormal. A significant fraction of embryos contain very few nuclei. About two-thirds of eggs with less than 5 nuclei are not fertilised.

      (Lane et al., 2000)
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (4)
      Reported As
      Symbol Synonym
      Cdk2hs.PS
      cdc2chs.P
      cdc2chs.PS
      Name Synonyms
      heat shock construct of Stern
      (Stern et al., 1993)
      Secondary FlyBase IDs
        References (4)