FlyBase Allele Report: Dmel\faf[FO8]

General Information

Symbol

Dmel\faf^FO8

Species

D. melanogaster

Name

FlyBase ID

FBal0031258

Feature type

allele

Associated gene

Dmel\faf

Associated Insertion(s)

Carried in Construct

Also Known As

faf^F08

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Fischer-Vize et al., 1992)

Mutagen

ethyl methanesulfonate

(Fischer-Vize et al., 1992)

Progenitor genotype

Cytology

Description

Nucleotide substitution: C?T. Amino acid replacement: H1986Y.

(Chen and Fischer, 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:31,785,557..31,785,557 [+]

Nucleotide change:

C31785557T

Reported nucleotide change:

C?T

Amino acid change:

Reported amino acid change:

H1986Y

(Chen and Fischer, 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cone cell (with faf^BX3)

(Chen et al., 2000)

denticle (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

denticle (with faf^B3)

(Stinchfield et al., 2012)

embryo | embryonic stage 4 | maternal effect (with faf^BX3)

(Chen et al., 2000)

embryonic/first instar larval cuticle (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

embryonic/first instar larval cuticle (with faf^B3)

(Stinchfield et al., 2012)

embryonic/first instar larval cuticle | non-rescuable maternal effect (with faf^B4)

(Stinchfield et al., 2012)

embryonic head | non-rescuable maternal effect (with faf^B4)

(Stinchfield et al., 2012)

eye (with faf^BX4)

(Chen and Fischer, 2000)

eye photoreceptor cell

(Li et al., 1997)

eye photoreceptor cell (with faf^BX4)

(Chen et al., 2000)

eye photoreceptor cell & ommatidium | ectopic | somatic clone

(Overstreet et al., 2004)

eye photoreceptor cell | increased number

(Chen et al., 2000)

eye photoreceptor cell | increased number (with faf^BX3)

(Chen et al., 2000)

eye photoreceptor cell | increased number (with faf^BX4)

(Chen and Fischer, 2000)

filzkorper (with Df(3R)faf-BP)

(Stinchfield et al., 2012)

filzkorper (with faf^B3)

(Stinchfield et al., 2012)

filzkorper | non-rescuable maternal effect (with faf^B4)

(Stinchfield et al., 2012)

ommatidium

(Chen et al., 2000)

ommatidium (with faf^BX3)

(Chen et al., 2000)

ommatidium (with faf^BX4)

(Chen and Fischer, 2000, Chen et al., 2000)

ventral denticle belt | ectopic (with faf^B3)

(Stinchfield et al., 2012)

ventral denticle belt | ectopic | non-rescuable maternal effect (with faf^B4)

(Stinchfield et al., 2012)

Detailed Description

Statement

Reference

Only 27% of the expected number of faf^FO8/faf^B3 zygotic mutants survive. The embryos have a ventralised cuticle with dorsally extended denticles and an ectopic denticle belt replacing the Filzkorper.

faf^FO8/Df(3R)faf-BP embryos have a ventralised cuticle with dorsally extended denticles and poorly developed Filzkorper.

Embryos derived from faf^B4/faf^FO8 females mated to wild-type males show significant ventralisation of the cuticle, with a herniated head, defective Filzkorper, malformed dorsal cuticle and denticle belts that extend well into the dorsal half of the embryo.

(Stinchfield et al., 2012)

faf^FO8 homozygous somatic clones in the eye lead to the formation of ommatidia containing one additional photoreceptor cell. For an ommatidium to show this phenotype, it is sufficient for 2 of the photoreceptors R2 to R5 to be mutant.

(Overstreet et al., 2004)

faf^BX3/faf^FO8 eyes are nearly wild type.

(Cadavid et al., 2000)

The external surface of the eye is irregular in faf^FO8/faf^BX4 flies and most facets contain more than the wild-type number of photoreceptor cells.

(Chen and Fischer, 2000)

faf^BX4/faf^FO8 flies have a rough eye phenotype, due to the ommatidia containing more than the normal number of photoreceptor cells. Embryos derived from homozygous females die prior to cellularisation. faf^BX3/faf^FO8 flies have facets with more than the wild-type number of photoreceptor cell and an abnormal number of cone cells.

(Chen et al., 2000)

Each facet contains more than the normal number of photoreceptors in mutant flies. BrdU incorporation resembles wild type in faf^FO8/faf^BX4 third larval instar eye discs.

(Chen et al., 2000)

faf^FO8/faf^BX3 flies have essentially wild-type eyes.

(Wu et al., 1999)

faf^BX3/faf^FO8 adults exhibit nearly wild type eyes with a smooth exterior.

(Fischer et al., 1997)

Most facets of transheterozygotes with faf^BX3 contain more than the normal complement of 8 photoreceptor cells, many facets have extra R7 cells and a small proportion have missing R7 cells.

(Li et al., 1997)

Mutation causes no significant alteration to the variegated eye phenotype of In(1)w^m4h.

(Henchoz et al., 1996)

Mutation has no effect on rho^hs.sev rough eye phenotype.

(Freeman, 1994)

Eyes roughened due to abnormalities in the hexagonal facet array: most facets include 1-3 extra outer photoreceptor cells which are probably mystery cells. Ovaries of homozygous females are normal in appearance but the embryos produced never form syncytial blastoderm and all fail to hatch.

(Fischer-Vize et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

faf^FO8/faf^BX3 has visible phenotype, enhanceable by shi¹/shi¹

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has visible phenotype, enhanceable by shi²/shi²

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has visible phenotype, enhanceable by lqf^AG/lqf[+]

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has visible phenotype, enhanceable by Chc[+]/Chc¹

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has visible phenotype, enhanceable by shi^EM42/shi[+]

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has visible phenotype, enhanceable by shi[+]/shi^EM45

(Cadavid et al., 2000)

Suppressed by

Statement

Reference

dpp^hr4/dpp[+], faf^FO8 has lethal | dominant | maternal effect | embryonic stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Med^UASp.cSa

(Stinchfield et al., 2012)

dpp^hr4/dpp[+], faf^FO8 has lethal | dominant | maternal effect | embryonic stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp

(Stinchfield et al., 2012)

faf^FO8/faf^BX3, lqf^bE25 has visible phenotype, suppressible by eff^XS347

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible phenotype, suppressible by eff⁰¹⁴⁶²

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible phenotype, suppressible by Prosβ6^rv10e

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible phenotype, suppressible by Prosβ6¹

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible | recessive phenotype, suppressible by eff^XS347

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible | recessive phenotype, suppressible by eff⁰¹⁴⁶²

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible | recessive phenotype, suppressible by Prosβ6^rv10e

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has visible | recessive phenotype, suppressible by Prosβ6¹

(Cadavid et al., 2000)

faf^FO8/faf^BX4 has visible phenotype, suppressible by Mmus\Usp9x^-.ro

(Chen et al., 2000)

faf^FO8/faf^BX4 has visible phenotype, suppressible by Mmus\Usp9x^+.ro

(Chen et al., 2000)

faf^FO8 has lethal | recessive | maternal effect | embryonic stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Mmus\Usp9x^+.UASp

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by Prim2^Sfaf240/DNAprim[+]

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by DNAprim[+]/Prim2^j10B2

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by Df(3L)rdgC-co2/+

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by S(faf)196¹⁹⁶/S(faf)196[+]

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by S(faf)244[+]/S(faf)244²⁴⁴

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by eff[+]/eff^Sfaf184

(Chen et al., 2000)

faf^FO8 has visible | recessive phenotype, suppressible by S(faf)86⁸⁶/S(faf)86[+]

(Chen et al., 2000)

NOT suppressed by

Statement

Reference

faf^FO8 has female sterile | recessive phenotype, non-suppressible by Prim2^Sfaf240/DNAprim[+]

(Chen et al., 2000)

faf^FO8 has female sterile | recessive phenotype, non-suppressible by S(faf)196¹⁹⁶/S(faf)196[+]

(Chen et al., 2000)

faf^FO8 has female sterile | recessive phenotype, non-suppressible by S(faf)244[+]/S(faf)244²⁴⁴

(Chen et al., 2000)

faf^FO8 has female sterile | recessive phenotype, non-suppressible by S(faf)86⁸⁶/S(faf)86[+]

(Chen et al., 2000)

faf^FO8 has female sterile | recessive phenotype, non-suppressible by eff[+]/eff^Sfaf184

(Chen et al., 2000)

Other

Statement

Reference

Med⁸, faf^FO8/faf[+] has partially lethal - majority die | dominant | maternal effect phenotype

(Stinchfield et al., 2012)

dpp^hr4/dpp[+], faf^FO8 has lethal | dominant | maternal effect | embryonic stage phenotype

(Stinchfield et al., 2012)

Phenotype Manifest In

Enhanced by

Statement

Reference

faf^FO8/faf^BX3 has eye phenotype, enhanceable by shi[+]/shi^EM45

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has eye phenotype, enhanceable by shi¹/shi¹

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has eye phenotype, enhanceable by shi²/shi²

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has eye phenotype, enhanceable by lqf^AG/lqf[+]

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has eye phenotype, enhanceable by Chc[+]/Chc¹

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has eye phenotype, enhanceable by shi^EM42/shi[+]

(Cadavid et al., 2000)

NOT Enhanced by

Statement

Reference

faf^FO8 has phenotype, non-enhanceable by Chc⁴

(Cadavid et al., 2000)

faf^FO8/faf^BX3 has ommatidium phenotype, non-enhanceable by arm¹/arm[+]

(Chen et al., 2000)

faf^FO8/faf^BX3 has ommatidium phenotype, non-enhanceable by arm⁴/arm[+]

(Chen et al., 2000)

faf^FO8/faf^BX3 has ommatidium phenotype, non-enhanceable by cno³/cno[+]

(Chen et al., 2000)

faf^FO8/faf^BX3 has ommatidium phenotype, non-enhanceable by cno²/cno[+]

(Chen et al., 2000)

Suppressed by

Statement

Reference

dpp^hr4/dpp[+], faf^FO8 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible | partially by Med^K738R.UASp

(Stinchfield et al., 2012)

dpp^hr4/dpp[+], faf^FO8 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Med^UASp.cSa

(Stinchfield et al., 2012)

dpp^hr4/dpp[+], faf^FO8 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Med^K738R.UASp

(Stinchfield et al., 2012)

faf^FO8/faf^BX3, lqf^bE25 has ommatidium phenotype, suppressible by eff^XS347

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has ommatidium phenotype, suppressible by eff⁰¹⁴⁶²

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has ommatidium phenotype, suppressible by Prosβ6^rv10e

(Cadavid et al., 2000)

faf^FO8/faf^BX3, lqf^bE25 has ommatidium phenotype, suppressible by Prosβ6¹

(Cadavid et al., 2000)

faf^FO8/faf^BX4 has eye phenotype, suppressible by lqf^+t19G

(Cadavid et al., 2000)

faf^FO8/faf^BX4 has eye phenotype, suppressible by lqf^ro.Tag:FLAG

(Cadavid et al., 2000)

faf^FO8/faf^BX4 has ommatidium phenotype, suppressible by Mmus\Usp9x^+.ro

(Chen et al., 2000)

faf^FO8/faf^BX4 has ommatidium phenotype, suppressible by Mmus\Usp9x^-.ro

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by Prim2^Sfaf240/DNAprim[+]

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by DNAprim[+]/Prim2^j10B2

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by Df(3L)rdgC-co2/+

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by S(faf)196¹⁹⁶/S(faf)196[+]

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by S(faf)244[+]/S(faf)244²⁴⁴

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by S(faf)86⁸⁶/S(faf)86[+]

(Chen et al., 2000)

faf^FO8 has ommatidium phenotype, suppressible by eff[+]/eff^Sfaf184

(Chen et al., 2000)

faf^FO8 has eye phenotype, suppressible by Scer\CDC34^ro.Tag:MYC

(Wu et al., 1999)

faf^FO8 has eye phenotype, suppressible by Scer\UBP2^{ro.hs.Tag:MYC}

(Wu et al., 1999)

faf^FO8 has eye phenotype, suppressible by eff^S98

(Wu et al., 1999)

faf^FO8 has eye phenotype, suppressible by eff^XS347

(Wu et al., 1999)

faf^FO8 has eye phenotype, suppressible by Prosβ6¹

(Huang et al., 1995)

NOT suppressed by

Statement

Reference

dpp^hr4/dpp[+], faf^FO8 has embryonic/first instar larval cuticle | maternal effect phenotype, non-suppressible by Med^UASp.cSa

(Stinchfield et al., 2012)

faf^FO8 has eye phenotype, non-suppressible by Scer\DOA4^ro.Tag:MYC

(Wu et al., 1999)

Enhancer of

Statement

Reference

faf^FO8 is an enhancer of eye photoreceptor cell phenotype of Rap1^E8

(Li et al., 1997)

faf^FO8 is an enhancer of eye photoreceptor cell phenotype of Rap1^E127

(Li et al., 1997)

NOT Enhancer of

Statement

Reference

faf^FO8/faf[+] is a non-enhancer of ommatidium phenotype of cno^mis1

(Chen et al., 2000)

faf^FO8/faf[+] is a non-enhancer of ommatidium phenotype of cno^mis1/cno²

(Chen et al., 2000)

Suppressor of

Statement

Reference

faf^FO8/faf[+] is a suppressor of embryonic/first instar larval cuticle phenotype of sog^Y506

(Stinchfield et al., 2012)

faf^FO8/faf[+] is a suppressor of denticle phenotype of sog^Y506

(Stinchfield et al., 2012)

faf^FO8/faf^BX4 is a suppressor of synaptic vesicle phenotype of hiw^unspecified

(DiAntonio et al., 2001)

NOT Suppressor of

Statement

Reference

faf^FO8/faf^BX4 is a non-suppressor of synapse phenotype of hiw^unspecified

(DiAntonio et al., 2001)

Other

Statement

Reference

dpp^hr4/dpp[+], faf^FO8 has embryonic/first instar larval cuticle | maternal effect phenotype

(Stinchfield et al., 2012)

faf^FO8, shi^EM45 has eye phenotype

(Cadavid et al., 2000)

eff^ro.Tag:MYC, faf^FO8/faf^BX3 has eye phenotype

(Wu et al., 1999)

Ras85D^bE382, faf^FO8 has eye photoreceptor cell phenotype

(Li et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos derived from faf^FO8/+ females mated to dpp^hr4/+ males show strong lethality (only 10% of the expected number survive). The embryos have a ventralised cuticle phenotype.

Embryos derived from Med⁸/+ females mated to males heterozygous for faf^FO8 show semi-lethality.

faf^FO8/+ partially suppresses the cuticle phenotype of sog^Y506 hemizygous embryos. The embryos have partially restored denticles, a normal body shape and poorly developed Filzkorper.

The survival of the progeny of faf^FO8/+ females mated to dpp^hr4/+ males is increased 1.6-fold by basal expression of Med^{Scer\UAS.P\T.cSa} (in the absence of a Scer\GAL4 driver) in the females, while basal expression of Med^{K738R.Scer\UAS.P\T} in the females increases survival of the progeny 4.2-fold. Expression of either Med^{Scer\UAS.P\T.cSa} or Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females increases survival of the progeny to approximately 80% in both cases.

The ventralised cuticle phenotype of embryos derived from faf^FO8/+ females mated to dpp^hr4/+ males is not rescued by basal expression of Med^{Scer\UAS.P\T.cSa} (in the absence of a Scer\GAL4 driver) in the females, while basal expression of Med^{K738R.Scer\UAS.P\T} in the females results in the rescue of the ventralised cuticle phenotype in some embryos. Expression of either Med^{Scer\UAS.P\T.cSa} or Med^{K738R.Scer\UAS.P\T} under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the females effectively rescues the ventralised cuticle phenotype of the embryos.

(Stinchfield et al., 2012)

The addition of either homozygous faf^BX4, or faf^BX4/faf^FO8 suppresses the physiological phenotype of hiw^unspecified, leading to a more tha doubling of both quantal content and mEJP frequency. However the synaptic overgrowth phenotype is not affected.

(DiAntonio et al., 2001)

The eyes of faf^BX3/faf^FO8 flies are severely malformed if the flies are also carrying one copy of lqf^AG. The faf^BX3/faf^FO8 phenotype is dominantly enhanced by Chc¹, no enhancement if seen with Chc⁴. The faf^BX3/faf^FO8 phenotype is dominantly enhanced by shi^EM42 or shi^EM45. The faf^BX3/faf^FO8 phenotype is slightly enhanced if the flies are also homozygous for shi¹ or shi². The lqf^bE25 faf^BX3/faf^FO8 mutant phenotype is suppressed by eff^XS347, eff⁰¹⁴⁶², Pros26^rv10e or Pros26¹. The faf^FO8/faf^BX4 eye phenotype is completely suppressed by one copy of lqf^+t19G or lqf^{ro.T:Zzzz\FLAG}.

(Cadavid et al., 2000)

The addition of eff^XS347 or eff^S98 dominantly suppresses the faf^FO8 eye phenotype. The addition of three copies of P{ro-UbcD1.T:Hsap\MYC} to faf^FO8/faf^BX3 flies enhances the eye phenotype.

(Wu et al., 1999)

Heterozygosity for Pros26¹ dramatically reduces the severity of the eye phenotype of faf^FO8 homozygotes.

(Huang et al., 1995)

Xenogenetic Interactions

Statement

Reference

Mmus\Usp9x^+.ro or Mmus\Usp9x^-.ro rescue nearly completely the eye phenotype of faf^BX4/faf^FO8 flies. Embryos derived from faf^FO8 females which are expressing Mmus\Usp9x^{+.Scer\UAS.P\T} in the germline under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} survive to adulthood and appear to be normal, fertile adults.