FlyBase Allele Report: Dmel\ord[5]

General Information

Symbol

Dmel\ord⁵

Species

D. melanogaster

Name

FlyBase ID

FBal0032314

Feature type

allele

Associated gene

Dmel\ord

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Miyazaki and Orr-Weaver, 1992)

Progenitor genotype

Cytology

Description

Amino acid replacement: K245term.

(Bickel et al., 1996)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:23,272,921..23,272,921 [-]

Nucleotide change:

A23272921T

Amino acid change:

K245term | ord-PA; K245term | ord-PB

Reported amino acid change:

K245term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Bickel et al., 1996)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Williams et al., 1996)

abnormal meiotic cell cycle | recessive

(Miyazaki and Orr-Weaver, 1992)

female semi-fertile

(Bickel et al., 1997)

female semi-sterile

(Meyer et al., 2010)

female sterile | recessive

(Miyazaki and Orr-Weaver, 1992)

Phenotype Manifest In

chromosome (with ord¹⁰)

(Radford et al., 2015)

meiosis & nuclear chromosome (with Df(2R)3-70)

(Balicky et al., 2002)

Detailed Description

Statement

Reference

ord⁵/ord¹⁰ mutant oocytes display loss of co-orientation of homologous chromosomes as compared to wild type, but do not display loss of centromere orientation towards the spindle pole.

(Radford et al., 2015)

ord⁵/Df(2R)3-70 germaria exhibit only a few synaptonemal complexes, all of which are abnormal. In most cases, distinct lateral elements are not found, with what appear to be twisted central elements containing organised transverse filaments in the absence of well-formed lateral elements along with some synaptonemal-like structures lack definition between the central region and what could be lateral elements. Recovery of the ring X chromosome R(1)2 relative to a normal rod X chromosome during female meiosis is significantly lowered in ord⁵/ord¹⁰ (ratio of ring/rod=0.2 compared to 0.7 in wild-type) mutants.

(Webber et al., 2004)

30% of nuclei show defects in sister chromatid cohesion in stage S5/6 ord⁵/Df(2R)3-70 spermatocytes.

(Balicky et al., 2002)

Sister-chromatid cohesion is lost during meiosis I. Single chromatids segregate aberrantly during both divisions, resulting in XY as well as XX gametes in males. XY gametes outnumber XX gametes by greater than threefold. Mutation does not interfere with ord⁴ activity: does not show negative complementation. In the absence of ord protein (ord¹⁰/ord⁵ transheterozygotes) sister chromatid cohesion is missing and individual chromatids segregate randomly to the poles with minimal chromosome loss.

(Bickel et al., 1997)

Precocious sister chromatid separation (PSCS) during prometaphase I, resulting in first and second division nondisjunction.

(Williams et al., 1996)

Increased frequency of premature sister chromatid disjunction. Only 12% of egg chambers have abnormal numbers of nurse cell nuclei.

(Miyazaki and Orr-Weaver, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Other

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has oocyte phenotype

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has chromosome | female | adult stage phenotype

(Gyuricza et al., 2016)

SA1^GL00534, Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰ has ovary phenotype

(Gyuricza et al., 2016)

SA1^GL00534, Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰ has female germline cell phenotype

(Gyuricza et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has ovary phenotype

(Gyuricza et al., 2016)

Scer\GAL4^{VP16.nanos.UTR}, ord⁵/ord¹⁰, vtd^GL00522 has female germline cell phenotype

(Gyuricza et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of either SA^GL00534 or vtd^GL00522 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} in the ord⁵/ord¹⁰ mutant background results in rudimentary ovaries lacking germ cells.

c(2)M^EP2115/c(2)M^Z0810,ord⁵/ord¹⁰ double mutants show defects in centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes relative to controls.

(Gyuricza et al., 2016)

Crossover frequency in the X euchromatin (pn-f interval) is reduced 65-fold in solo^Z2-3534 ord^Z5736/solo^Z2-0198 ord⁵ females compared to doubly heterozygous sibling controls. This reduction is intermediate in value between those of ord or solo single mutants.

(Yan and McKee, 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Strong ord allele. Does not interact with ord⁴ to alter the male or female meiotic chromosome segregation phenotype.

(Bickel et al., 1996)

Strong ord mutation.

(Bickel et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

ord⁵

(Das et al., 2016, Gyuricza et al., 2016, Radford et al., 2015, Yan and McKee, 2013, Meyer et al., 2010, Yan et al., 2010, Page et al., 2008)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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