FlyBase Allele Report: Dmel\c(2)M[EP2115]

General Information

Symbol

Dmel\c(2)M^EP2115

Species

D. melanogaster

Name

FlyBase ID

FBal0121689

Feature type

allele

Associated gene

Dmel\c(2)M

Associated Insertion(s)

P{EP}c(2)M^EP2115

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

loss of function allele

(Manheim and McKim, 2003)

Mutagen

P-element activity

(Manheim and McKim, 2003, Gene Disruption Project members, 2001-, McKim, 2001.3.5)

Progenitor genotype

Associated Insertion(s)

P{EP}c(2)M^EP2115

Cytology

Description

Insertion in the coding region.

(Heidmann et al., 2004)

The P{EP}c(2)M^EP2115 insertion is 61 amino acids from ATG within the third exon.

(Manheim and McKim, 2003)

Insertion of P{EP} into the c(2)M coding region.

(McKim, 2001.3.5)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:16,304,037..16,304,037 [-]

(Kavi et al., 2015, Heidmann et al., 2004, Manheim and McKim, 2003, Gene Disruption Project members, 2001-, McKim, 2001.3.5)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal meiotic cell cycle

(Manheim and McKim, 2003)

Phenotype Manifest In

female germline cyst | oogenesis stage S1

(Hughes et al., 2019)

oocyte (with c(2)M^Z0810)

(Gyuricza et al., 2016)

stage S1 oocyte | increased number

(Joyce and McKim, 2010)

synaptonemal complex | adult stage | female (with c(2)M^Z0810)

(Gyuricza et al., 2016)

synaptonemal complex | oogenesis stage S1

(Hughes et al., 2019)

Detailed Description

Statement

Reference

In c(2)M^EP2115 germaria, the synaptonemal complex begins to load as small patches near the centromeres and multiple additional locations, but fails to elongate the central region components into full tracks of synaptonemal complex.

(Hughes et al., 2019)

Oocytes of c(2)M^EP2115/c(2)M^Z0810 transheterozygous mutants display defects in synaptonemal complex assembly, while the centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes is not affected.

(Gyuricza et al., 2016)

Mutant females show a dramatic elevation in X chromosome non-disjunction during meiosis compared to controls.

(Heidmann et al., 2004)

Homozygotes exhibit a high rate on non-disjunction (29.3% X chromosome non-disjunction) and a reduced number of crossovers, with the number of crossovers per physical distance diminishing as the region approaches the centromere. When irradiated c(2)M^Z0810 females do not show a large increase in crossing over levels.

(Manheim and McKim, 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

c(2)M^EP2115, mei-W68⁴⁵⁷² has abnormal meiotic cell cycle phenotype

(Manheim and McKim, 2003)

Phenotype Manifest In

Suppressed by

Statement

Reference

c(2)M^EP2115 has stage S1 oocyte | increased number phenotype, suppressible by pch2^EY01788a

(Joyce and McKim, 2010)

NOT suppressed by

Statement

Reference

c(2)M^EP2115 has stage S1 oocyte | increased number phenotype, non-suppressible by c(3)G⁶⁸

(Joyce and McKim, 2010)

c(2)M^EP2115 has stage S1 oocyte | increased number phenotype, non-suppressible by mei-218¹

(Joyce and McKim, 2010)

Suppressor of

Statement

Reference

c(2)M^EP2115/c(2)M^EP2115 is a suppressor | partially of female germline cyst | oogenesis phenotype of sina^A4

(Hughes et al., 2019)

c(2)M^EP2115/c(2)M^EP2115 is a suppressor | partially of female germline cyst | oogenesis stage S1 phenotype of Df(3L)sina^SH, sina^A4

(Hughes et al., 2019)

c(2)M^EP2115/c(2)M^EP2115 is a suppressor | partially of synaptonemal complex | oogenesis stage S1 phenotype of sina^A4

(Hughes et al., 2019)

c(2)M^EP2115/c(2)M^EP2115 is a suppressor | partially of synaptonemal complex | oogenesis phenotype of Df(3L)sina^SH, sina^A4

(Hughes et al., 2019)

c(2)M^Z0810/c(2)M^EP2115 is a suppressor of oocyte phenotype of Nipped-B^GL00574

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115 is a suppressor of synaptonemal complex | female | adult stage phenotype of Nipped-B^GL00574

(Gyuricza et al., 2016)

NOT Suppressor of

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115 is a non-suppressor of oocyte phenotype of SA1^GL00534

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115 is a non-suppressor of synaptonemal complex | female | adult stage phenotype of SA1^GL00534

(Gyuricza et al., 2016)

Other

Statement

Reference

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has oocyte phenotype

(Gyuricza et al., 2016)

c(2)M^Z0810/c(2)M^EP2115, ord⁵/ord¹⁰ has chromosome | female | adult stage phenotype

(Gyuricza et al., 2016)

Df(3L)ED4470/sunn^167-109, c(2)M^Z0810/c(2)M^EP2115 has ovary phenotype

(Gyuricza et al., 2016)

Df(3L)ED4470/sunn^167-109, c(2)M^Z0810/c(2)M^EP2115 has synaptonemal complex | female | adult stage phenotype

(Gyuricza et al., 2016)

Additional Comments

Genetic Interactions

Statement

Reference

Combination with c(2)M^EP2115/c(2)M^Z0810 in transheterozygous state cannot suppress the decrease in average number of c(3)G-positive patches in oocytes between regions 2a and 3 of the germarium observed in flies expressing SA^GL00534 under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} but it does ameliorate the reduction in patches number observed in flies expressing Nipped-B^GL00574.

sunn^167-109/Df(3L)ED4470;c(2)M^EP2115/c(2)M^Z0810 double mutant flies do not show any evidence of synaptonemal complex assembly in oocytes.

c(2)M^EP2115/c(2)M^Z0810,ord⁵/ord¹⁰ double mutants show defects in centromere clustering and sister chromatid cohesion (assessed by number of cid foci) in pachytene oocytes relative to controls.

(Gyuricza et al., 2016)

c(2)M^EP2115, mei-W68⁴⁵⁷² double mutant mothers show a decrease in crossovers in most chromosomal regions, though not in the st-cu region.

(Manheim and McKim, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

c(2)M^10C

(Page et al., 2007)

c(2)M^8C

(Page et al., 2007)

c(2)M^Z0810

(Manheim and McKim, 2003)

c(2)M^Z3780

(Manheim and McKim, 2003)

Rescued by

c(2)M^Z0810/c(2)M^EP2115 is rescued by Scer\GAL4^{VP16.nanos.UTR}/c(2)M^UASp.Tag:HA

(Gyuricza et al., 2016)

c(2)M^EP2115 is rescued by c(2)M^-FRT.Tag:MYC

(Heidmann et al., 2004)

c(2)M^EP2115 is rescued by c(2)M^{csm1-3.Tag:MYC}

(Heidmann et al., 2004)

Partially rescued by

c(2)M^Z0810/c(2)M^EP2115 is partially rescued by c(2)M^UASp.Tag:HA/Scer\GAL4^hs.PB

(Gyuricza et al., 2016)

Not rescued by

c(2)M^Z0810/c(2)M^EP2115 is not rescued by c(2)M^UASp.Tag:HA/Scer\GAL4^VP16.bam

(Gyuricza et al., 2016)

Comments

A 1hr heat-shock induced pulse of expression of c(2)M^{Scer\UAS.P\T.T:Ivir\HA1} (controlled by Scer\GAL4^hs.PB) partially reduced the chromosome nondisjunction frequency in the eggs laid 7-10 days after the heat-shock (these would have been in the germarium at the time of the heat-shock), Scer\GAL4^{bam.T:Hsim\VP16}-driven expression of c(2)M^{Scer\UAS.P\T.T:Ivir\HA1} fails to rescue the nondisjunction phenotype, in contrast to expression driven by Scer\GAL4^{nos.UTR.T:Hsim\VP16} which results in full rescue.

(Gyuricza et al., 2016)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

17233

w¹¹¹⁸; P{EP}c(2)M^EP2115

Notes on Origin

Discoverer

Comments

Phenotypic analysis (non-disjunction) provides an allelic series: c(2)M^EP2115 > c(2)M^Z0810 > c(2)M^Z3780.

(Manheim and McKim, 2003)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

C(2)M^EP2115

(Pek et al., 2012)

c(2)M(EP2115)

(Hughes et al., 2019)

c(2)M^EP2115

(Lehner, 2020.12.4, Gyuricza et al., 2016, Urban et al., 2014, Page et al., 2008, Page et al., 2007, Heidmann et al., 2004)

c(2)M^EP

(Joyce and McKim, 2010)

mei-910^EP2115

(McKim, 2001.3.5)

Name Synonyms

Secondary FlyBase IDs

References (13)

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