FB2024_03 , released June 25, 2024
Allele: Dmel\rox63
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General Information
Symbol
Dmel\rox63
Species
D. melanogaster
Name
FlyBase ID
FBal0032585
Feature type
allele
Associated gene
Dmel\ro
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Dokucu et al., 1996, Freeman et al., 1992, Heberlein et al., 1991, van Vactor et al., 1991)

loss of function allele

(DeMille et al., 1996, Kimmel et al., 1990)
Mutagen
X ray
(Heberlein et al., 1991, Kimmel et al., 1990)
Progenitor genotype
Cytology
Description

Contains a 20bp deletion near the beginning of the ro protein-coding region.

(Heberlein and Rubin, 1991)

22bp deletion that results in a frame shift after amino acid 30.

(Heberlein et al., 1991)

A small deletion in the 5' end of the coding region that results in a frameshift after the 31st codon.

(Kimmel et al., 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Twinning of R8 photoreceptor cells is seen in mutant animals.

      (Rawlins et al., 2003)

      Ommatidia contain 2-3 R8 cells, ectopic R8 cells arise from the R8 equivalence group.

      (Dokucu et al., 1996)

      Eyes from homozygous rox63 flies are slightly reduced in size, the ommatidia are disorganised and usually contain only 6 photoreceptors. Eyes from rox63/rox63, S126/+ double mutant flies are severely reduced in size and disorganised, and individual ommatidia contain only 3-4 photoreceptors.

      (Heberlein and Rubin, 1991)

      Compound eyes are smaller than wild type and arrangement of the facets is disorganised.

      (Heberlein et al., 1991)

      Eye discs contain well-spaced clusters of cells with apically disposed nuclei, the number and arrangement of the cells is grossly abnormal. More than one boss-expressing cell was observed within many clusters. Prior to row 6 many cells per cluster contain boss-immunoreactive multivesicular bodies, boss is internalized as soon as boss expression is present on an adjacent cell surface. This correlates with a decrease in the number of R1-R6 neurons and an increase in the number of R7 neurons.

      (van Vactor et al., 1991)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressor of
      Statement
      Reference

      rox63 is a suppressor of photoreceptor cell R8 | somatic clone | cell non-autonomous phenotype of sensE1/sensE2

      (Frankfort et al., 2001)
      Other
      Statement
      Reference

      rox63, svp1 has ommatidium phenotype

      (Heberlein et al., 1991)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      In rho7M43; ru1; sensE2 triple mutant clones, no photoreceptors differentiate except for a few photoreceptors near the clonal boundary, presumably rescued non-autonomously by neighboring wild-type cell. No rescue of photoreceptor development is seen when these triple mutant clones are made in a rox63 homozygous background.

      (Frankfort and Mardon, 2004)

      Double mutant clones with svp1, in otherwise wild type ommatidia, contain only central type receptor cells. The overall number of photoreceptor cells in each ommatidium is the same in svp-,ro- and svp+,ro- clones.

      (Heberlein et al., 1991)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      rox63 is rescued by roUAS.cFa/Scer\GAL4hs.PB

      (Frankfort and Mardon, 2004)
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      Comments
      Comments

      svp expression is derepressed in the eye discs in cells at the position of the R2 and/or R5 cells.

      (Heberlein et al., 1991)

      No interaction with P{sev-svp1} or P{sev-svp2} exists.

      (Begemann et al., 1995)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      roX63
      (Frankfort and Mardon, 2004, Rawlins et al., 2003, Wernet et al., 2003, Begemann et al., 1995, Moses and Rubin, 1991)
      rox63
      (Iwanami et al., 2005)
      Name Synonyms
      Secondary FlyBase IDs
        References (17)