FB2024_03 , released June 25, 2024
Allele: Dmel\Syt1D27
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General Information
Symbol
Dmel\Syt1D27
Species
D. melanogaster
Name
FlyBase ID
FBal0032848
Feature type
allele
Associated gene
Dmel\Syt1
Associated Insertion(s)
Carried in Construct
Key Links
Allele class

amorphic allele - genetic evidence

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(DiAntonio and Schwarz, 1994, DiAntonio et al., 1993)
Mutagen
gamma ray
(DiAntonio et al., 1993)
ethyl methanesulfonate
(DiAntonio and Schwarz, 1994)
Progenitor genotype
Caused by aberration
In(2)sytD27
Cytology
Description

Inversion breaks in the middle of the syt coding region.

(DiAntonio and Schwarz, 1994)

Breakpoint within syt gene, mapping between amino acids 303 and 322.

(DiAntonio et al., 1993)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Syt1AD4/Syt1D27 synapses show decreased evoked neurotransmitter release compared to wild-type controls. They also show a significantly slower time constant of endocytosis.

      Syt1AD4/Syt1D27 synapses contain vesicles with a modest increase in diameter when at rest. After stimulation, there is a dramatic further increase in average vesicle diameter. Inspection of individual active zones demonstrates that vesicular structures with diameters greater than 10nm are observed throughout the vesicle cluster, both at the active zone and periactive zone region. The retrieval of abnormally large vesicles is on going at a time when most endocytosis has been completed in controls.

      (Poskanzer et al., 2006)

      The excitatory junctional potential (EJP) amplitude at the neuromuscular junction is greatly reduced compared to wild type in sytAD4/sytD27 larvae.

      (Robinson et al., 2002)

      Many homozygous larvae hatch from the egg case (though well after the normal period) and show motor activity such as crawling and feeding. Transheterozygotes with Df(2L)DTD2 never survive to adulthood.

      (DiAntonio and Schwarz, 1994)

      Transheterozygote combinations with other syt alleles are lethal.

      (Littleton et al., 1994)

      Sluggish crawling and feeding behaviour. Larvae do not grow or moult as wild type larvae would, and die after a week (at 18oC).

      (DiAntonio et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      The viability of sytD27/sytAD4 flies (rescued to adulthood by sytelav.PD) is significantly reduced in combination with stnA6/Y or stnA7/Y. The few surviving males have an extreme behavioural phenotype and are very sedentary and severely uncoordinated (they are more uncoordinated than stnA+/Y ; sytD27/sytAD4 flies rescued to adulthood by sytelav.PD). They die within 48 hours of eclosion. sytD27/sytAD4 females (rescued to adulthood by sytelav.PD) and also heterozygous for stnA6 show abnormal behaviour, including a spread leg "spider-like" appearance and a slightly uncoordinated gait.

      (Phillips et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescued by

      Syt1D27/Syt1AD4 is rescued by Scer\GAL4elav-C155/Syt1UAS.Tag:CALI(4C)

      (Marek and Davis, 2002)

      Syt1D27/Syt1AD4 is rescued by Syt1elav.PD

      (Phillips et al., 2000)
      Partially rescued by

      Syt1D27/Syt1AD4 is partially rescued by Syt1UAS.Tag:HA/Scer\GAL4hs.PB

      (Robinson et al., 2002)

      Syt1D27/Syt1AD4 is partially rescued by Syt1D2N.UAS.Tag:HA/Scer\GAL4elav.PLu

      (Robinson et al., 2002)

      Syt1D27/Syt1AD4 is partially rescued by Syt1D2N.UAS.Tag:HA/Scer\GAL4hs.PB

      (Robinson et al., 2002)

      Syt1D27 is partially rescued by Syt1elav.PD

      (DiAntonio and Schwarz, 1994)

      Syt1D27 is partially rescued by Syt1elav.PD

      (DiAntonio et al., 1993)
      Comments

      Homozygous lethality can be rescued by sytelav.PD. Rescued flies that emerge are lethargic and show an electrophysiological deficit due to insufficient syt. A second copy of sytelav.PD improves viability and motor activity. ERGs of mutants carrying one copy of sytelav.PD lack both on- and off-transients suggesting a disruption in synaptic communication. In mutants the evoked neurotransmitter release is decreased and spontaneous release in increased (intracellular recordings made from muscle 6).

      (DiAntonio and Schwarz, 1994)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Mutant phenotype can be rescued, when homozygous or in trans to sytN6, by sytelav.PD.

      (Littleton et al., 1994)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Syt1D27
      (Poskanzer et al., 2006)
      sytD27
      sytD27
      (Phillips et al., 2000)
      Name Synonyms
      Secondary FlyBase IDs
        References (8)