FlyBase Allele Report: Dmel\zfh1[2]

General Information

Symbol

Dmel\zfh1²

Species

D. melanogaster

Name

FlyBase ID

FBal0033228

Feature type

allele

Associated gene

Dmel\zfh1

Associated Insertion(s)

Carried in Construct

Also Known As

zfh-1²

Key Links

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Broihier et al., 1998)

loss of function allele

(Lai et al., 1993)

Mutagen

ethyl methanesulfonate

(Lai et al., 1993)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Lai et al., 1993)

some die during embryonic stage | recessive

(Lai et al., 1993)

Phenotype Manifest In

Detailed Description

Statement

Reference

There are no hematopoietic cells in the lymph gland of zfh1² mutant embryos.

(Frandsen et al., 2008)

zfh1²/Df(3R)GC14 mutant embryos exhibit severe disruption to somatic muscle patterning, with little apparent organisation of muscles in the ventral and lateral regions.

(Clark et al., 2006)

Mutant embryos lack the eve-positive pericardial cells from the linear heart tube. The cardiac myocytes are present in the normal number, but they are abnormally aligned, resulting in a distorted heart tube.

(Liu et al., 2006)

Early steps in mesoderm differentiation, such as the formation of somatic, visceral and cardiac mesoderm appears normal in zfh1² embryos. The eve-expressing pericardial cells (EPCs) are missing. The majority of the myocardial and the pericardial cells are present in later embryos, but the heart structure has minor to moderate defects. Some of the body wall muscles are defective or missing.

(Su et al., 1999)

Embryonic cuticle normal. Somatic musculature shows variable patterning and insertion site positioning defects, principally in the ventral oblique and the dorsal muscles. These pattern defects are evident at the segregation of the muscle precursors, as revealed by an aberrant pattern of S59 gene expression. Midgut development is abnormal: constrictions rarely complete the subdivision of the yolk. Variable heart defects are evident as kinked or discontinuous heart. Examination with antibody to the vasa gene product pole cell marker reveal that the shape of the gonads is also abnormal. The adult muscle precursors in the embryo, identified as persistant twist-expressing cells, show variable abnormalities of number and position: up to 25% of the adult muscle precursor cells can be missing.

(Lai et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

zfh1² has embryonic/larval pericardial cell phenotype, suppressible by Scer\GAL4^twi.PG/Scer\GAL4^how-24B

(Liu et al., 2006)

zfh1² has cardioblast phenotype, suppressible by Scer\GAL4^twi.PG/Scer\GAL4^how-24B

(Liu et al., 2006)

zfh1² has embryonic/larval dorsal vessel phenotype, suppressible by Scer\GAL4^twi.PG/Scer\GAL4^how-24B

(Liu et al., 2006)

zfh1² has pericardial cell phenotype, suppressible by Scer\GAL4^twi.PG/eve^UAS.cBa

(Su et al., 1999)

NOT suppressed by

Statement

Reference

zfh1² has embryonic/larval lymph gland | embryonic stage phenotype, non-suppressible by dpp^d6

(Frandsen et al., 2008)

zfh1² has hemocyte | embryonic stage phenotype, non-suppressible by dpp^d6

(Frandsen et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

There are no hematopoietic cells in the lymph gland of dpp^d6, zfh1² double mutant embryos.

(Frandsen et al., 2008)

The number of eve-expressing mesodermal cells per hemisegment is similar in stg² single mutant and stg²; zfh1² double mutant embryos. numb² ; zfh1² double mutant embryos have no eve-expressing pericardial cells (EPCs) and no DA1 muscles, except for rarely 1 or 2 EPCs are present. spi¹ ; zfh1² embryos have no EPCs and no DA1 muscles. Ectopic expression of eve^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG in a zfh1² background restores the formation of some EPCs.

(Su et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

zfh1² is rescued by Scer\GAL4^how-24B/zfh1^UAS.cPa

(Frandsen et al., 2008)

zfh1² is rescued by Scer\GAL4^twi.PG/Scer\GAL4^how-24B/zfh1^UAS.cLb

(Liu et al., 2006)

Comments

Expression of zfh1^Scer\UAS.cPa under the control of the pan-mesodermal driver Scer\GAL4^how-24B in zfh1² mutants rescues the lymph gland phenotype.

(Frandsen et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

90314

zfh1²/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

Comments

Identification of zfh1 alleles rests on several alleles of this complementation group having no detectable zfh1 protein. Phenotype of zfh1² over deficiciency for zfh1 not described.

(Lai et al., 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

l(3)100Ac

(Lai et al., 1993)

zfh-1²

(Lee and Lundell, 2007, Clark et al., 2006, Liu et al., 2006)

zfh1²

(Wu et al., 2019, Ismat et al., 2010, Frandsen et al., 2008, Johnson et al., 2007, Garces and Thor, 2006, Layden et al., 2006)

Name Synonyms

Secondary FlyBase IDs

References (14)

Report Sections

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