FlyBase Allele Report: Dmel\eve[UAS.cBa]

General Information

Symbol

Dmel\eve^UAS.cBa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Brand

FlyBase ID

FBal0031220

Feature type

allele

Associated gene

Dmel\eve

Associated Insertion(s)

Carried in Construct

P{UAS-eve.B}

Also Known As

UAS-eve

Key Links

Transgenic product class

wild_type

(Brand and Perrimon, 1993)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Brand and Perrimon, 1993)

Mutagen

in vitro construct

(Brand and Perrimon, 1993)

Progenitor genotype

Carried in construct

P{UAS-eve.B}

Cytology

Description

UASt regulatory sequences drive expression of a cDNA-genomic DNA fusion encoding eve.

(Brand and Perrimon, 1993)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

eve

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | gastrula stage | somatic clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Zallen and Wieschaus, 2004)

abnormal neuroanatomy, with Scer\GAL4^eg-Mz360

(Zarin et al., 2014)

abnormal neuroanatomy, with Scer\GAL4^elav.PLu

(Labrador et al., 2005)

abnormal neuroanatomy, with Scer\GAL4^Vap.P0201

(Garces and Thor, 2006)

lethal - all die during embryonic stage, with Scer\GAL4^h-1J3

(Brand and Perrimon, 1993)

lethal - all die during embryonic stage, with Scer\GAL4^how-24B

(Brand and Perrimon, 1993)

lethal - all die during embryonic stage, with Scer\GAL4^{l(3)31-1-31-1}

(Brand and Perrimon, 1993)

some die during embryonic stage, with Scer\GAL4^h-1J3

(Brand and Perrimon, 1993)

some die during embryonic stage, with Scer\GAL4^how-24B

(Brand and Perrimon, 1993)

some die during embryonic stage, with Scer\GAL4^{l(3)31-1-31-1}

(Brand and Perrimon, 1993)

Phenotype Manifest In

abdominal 3 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 3 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 3 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 3 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 4 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 4 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 4 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 1 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 2 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 5 lateral transverse muscle 4 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal 5 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal 5 ventral longitudinal muscle 3 & neuromuscular junction, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 3, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 3, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 4, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 4, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 5, with Scer\GAL4^elav-C155

(Landgraf et al., 1999)

abdominal posterior fascicle & abdominal segment 5, with Scer\GAL4^ftz.ng

(Landgraf et al., 1999)

cardiac outflow muscle cell, with Scer\GAL4^how-24B

(Zikova et al., 2003)

cardioblast, with Scer\GAL4^how-24B

(Jagla et al., 2002)

dMP2 neuron, with Scer\GAL4^Vap.P0201

(Garces and Thor, 2006)

dorsal transverse muscle cell, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

embryonic/larval heart anchoring cell, with Scer\GAL4^how-24B

(Zikova et al., 2003)

embryonic heart anchoring cell primordium, with Scer\GAL4^how-24B

(Zikova et al., 2003)

embryonic primordium of adult hindgut precursor, with Scer\GAL4^cad-em459

(Gorfinkiel et al., 1999)

germ band | gastrula stage | somatic clone, with Scer\GAL4^{VP16.mat.αTub67C}

(Zallen and Wieschaus, 2004)

heart primordium, with Scer\GAL4^how-24B

(Jagla et al., 2002)

larval abdominal segmental nerve, with Scer\GAL4^elav.PLu

(Labrador et al., 2005)

larval EW neuron, with Scer\GAL4^eg-Mz360

(Zarin et al., 2014)

larval intersegmental nerve, with Scer\GAL4^elav.PLu

(Labrador et al., 2005)

muscle cell of ventral acute muscle 2, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

nucleus | increased number, with Scer\GAL4^kirre.PR

(Bataillé et al., 2010)

presumptive embryonic/larval central nervous system, with Scer\GAL4^elav.PLu

(Broihier et al., 2004)

Detailed Description

Statement

Reference

Misexpression of eve^Scer\UAS.cBa in EW neurons, under the control of Scer\GAL4^eg-Mz360 transcriptionally reprograms EW interneurons to express the same array of molecules as in dorsal interneurons. These EW neurons no longer cross the CNS midline and remain ipsilateral, with some projecting towards the muscle field.

In almost all EW neurons, misexpressing eve^Scer\UAS.cBa axons fail to cross the midline and in 25% of hemisegments project laterally and join the motor roots.

(Zarin et al., 2014)

The number of nuclei resulting from the ectopic expression of eve^Scer\UAS.cBa in DT1 and VA2 muscles under the control of Scer\GAL4^kirre.PR increases from the wild-type number of 8 for DT1 and 9 for VA2 to numbers that are more characteristic of DA1.

(Bataillé et al., 2010)

Expression of eve^Scer\UAS.cBa in dMP2 neurons, under the control of Scer\GAL4^Vap.P0201 can trigger lateral ventral nerve cord exit of dMP2 at low frequency (approximately 5.5%).

(Garces and Thor, 2006)

Misexpression of eve^Scer\UAS.cBa, under the control of Scer\GAL4^elav.PLu, results in aberrant dorsal projection of both ventral-ISNb and lateral-SNa branches, leading to the joining of these branches with the dorsal branch of the ISN.

(Labrador et al., 2005)

Anterior-posterior polarization of cells in the extending germband is disrupted in eve^Scer\UAS.cBa; Scer\GAL4^{mat.αTub67C.T:Hsim\VP16} embryos. This disruption correlates with a failure of germband extension. (Note: The embryos analysed in these experiments were mosaics - due to the presence of lds^Hor-1, which induces the sporadic loss of paternally derived chromosomes).

(Zallen and Wieschaus, 2004)

In eve^Scer\UAS.cBa; Scer\GAL4^how-24B embryos, the cardiac outflow muscles do not attach to the tip of the heart, but instead extend anteriorly and attach to invaginating heart-anchoring cells (HANC), which are no longer able to anchor properly the tip of the heart. The main cardiac outflow muscle branch that normally attaches to the cardiac outflow region is absent or fused with the HANC-attaching branch. The same phenotypes are seen in eve^Scer\UAS.cBa; Scer\GAL4^tin.cBa embryos, albeit with lower penetrance.

(Zikova et al., 2003)

When eve^Scer\UAS.cBa is driven by Scer\GAL4^how-24B, a significant number if cells from the dorsal mesoderm assume altered identities. Losses of heart cells and cardioblasts are seen.

(Jagla et al., 2002)

eve^Scer\UAS.cBa when driven by Scer\GAL4^cad-em459 causes an enlargement of the adult hindgut primordium.

(Gorfinkiel et al., 1999)

When expression is driven by Scer\GAL4^elav-C155 or Scer\GAL4^ftz.ng, all peripheral nerve branches fuse. Ventral and lateral muscles left initially uninnervated eventually form neuromuscular junctions, though the position of the neuromuscular junctions on the muscles is often abnormal. The delayed-formation neuromuscular junctions show wild-type neuromuscular target recognition.

(Landgraf et al., 1999)

When eve is expressed in the expression pattern of h using Scer\GAL4^h-1J3, the cuticles prepared from the dead embryos exhibit extra denticles between alternating segments, in regions that are normally naked.

(Brand and Perrimon, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^eg-Mz360, eve^UAS.cBa has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^eg-Mz360/zfh1^UAS.cUa

(Zarin et al., 2014)

Scer\GAL4^eg-Mz360, eve^UAS.cBa has abnormal neuroanatomy phenotype, enhanceable by Scer\GAL4^eg-Mz360/grn^UAS.cZa

(Zarin et al., 2014)

Scer\GAL4^Vap.P0201, eve^UAS.cBa has abnormal neuroanatomy phenotype, enhanceable by grn^UAS.cBa/Scer\GAL4^Vap.P0201

(Garces and Thor, 2006)

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, eve^UAS.cBa has abnormal neuroanatomy phenotype, suppressible | partially by unc-5⁸

(Zarin et al., 2014)

Scer\GAL4^eg-Mz360, eve^UAS.cBa has abnormal neuroanatomy phenotype, suppressible | partially by beat-Ia^C163/unc-5⁸/beat-Ia³

(Zarin et al., 2014)

Scer\GAL4^elav-C155, eve^UAS.cBa has abnormal neuroanatomy phenotype, suppressible | partially by Scer\GAL4^elav-C155/beat-Ia^UAS.cFa

(Landgraf et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^eg-Mz360/eve^UAS.cBa is an enhancer of abnormal neuroanatomy phenotype of Scer\GAL4^eg-Mz360, zfh1^UAS.cUa

(Zarin et al., 2014)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^eg-Mz360, eve^UAS.cBa has larval EW neuron phenotype, enhanceable by Scer\GAL4^eg-Mz360/zfh1^UAS.cUa

(Zarin et al., 2014)

Scer\GAL4^eg-Mz360, eve^UAS.cBa has larval EW neuron phenotype, enhanceable by Scer\GAL4^eg-Mz360/grn^UAS.cZa

(Zarin et al., 2014)

Scer\GAL4^Vap.P0201, eve^UAS.cBa has dMP2 neuron phenotype, enhanceable by grn^UAS.cBa/Scer\GAL4^Vap.P0201

(Garces and Thor, 2006)

Suppressed by

Statement

Reference

Scer\GAL4^eg-Mz360, eve^UAS.cBa has larval EW neuron phenotype, suppressible | partially by unc-5⁸

(Zarin et al., 2014)

Scer\GAL4^eg-Mz360, eve^UAS.cBa has larval EW neuron phenotype, suppressible | partially by beat-Ia^C163/unc-5⁸/beat-Ia³

(Zarin et al., 2014)

Enhancer of

Statement

Reference

Scer\GAL4^eg-Mz360/eve^UAS.cBa is an enhancer of larval EW neuron phenotype of Scer\GAL4^eg-Mz360, zfh1^UAS.cUa

(Zarin et al., 2014)

Suppressor of

Statement

Reference

eve^UAS.cBa/Scer\GAL4^eve.eme is a suppressor of embryonic/larval pericardial cell | larval stage phenotype of lbe^eve.SS

(Buechling et al., 2009)

eve^UAS.cBa/Scer\GAL4^eve.eme is a suppressor of adult heart phenotype of lbe^eve.SS

(Buechling et al., 2009)

eve^UAS.cBa/Scer\GAL4^eve.eme is a suppressor of adult heart muscle cell phenotype of lbe^eve.SS

(Buechling et al., 2009)

Scer\GAL4^twi.PG/eve^UAS.cBa is a suppressor of pericardial cell phenotype of zfh1²

(Su et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

CNS exit from EW neurons misexpressing eve^Scer\UAS.cBa is partially suppressed in an unc-5⁸ mutant background and midline crossing is partially restored.

CNS exit from EW neurons misexpressing eve^Scer\UAS.cBa is partially suppressed in an unc-5⁸ beat-Ia^C163/beat-Ia³ mutant background while midline crossing is partially restored (in 15% of hemisegments).

Combined expression of eve^Scer\UAS.cBa with zfh1^Scer\UAS.cZa in EW neurons, under the control of Scer\GAL4^eg-Mz360, leads to a strong lateral exit of EW neurons in 80% of hemisegments.

Combined expression of eve^Scer\UAS.cBa with grn^Scer\UAS.cZa in EW neurons, under the control of Scer\GAL4^eg-Mz360, leads to a strong lateral exit of EW neurons in 72% of hemisegments.

(Zarin et al., 2014)

Scer\GAL4^eve.eme-mediated expression of eve^Scer\UAS.cBa rescues the reduced number of pericardial cells in lbe^eve.SS larvae.

Scer\GAL4^eve.eme-mediated expression of eve^Scer\UAS.cBa rescues the reduced heart diameter of lbe^eve.SS adults, and partially rescues the reduced diastolic and systolic diameters seen in live lbe^eve.SS hearts.

Scer\GAL4^eve.eme-mediated expression of eve^Scer\UAS.cBa rescues the myofibrillar structure defects of lbe^eve.SS adult hearts.

(Buechling et al., 2009)

Expression of grn^Scer\UAS.cBa and eve^Scer\UAS.cBa in dMP2 neurons, under the control of Scer\GAL4^Vap.P0201 enhances the eve^Scer\UAS.cBa dMP2 pathfinding defect, resulting in a high frequency of lateral exit out from the ventral nerve cord (in approximately 45% of cases).

(Garces and Thor, 2006)

The fusion of peripheral nerve branches with the ISN seen when eve^Scer\UAS.cBa expression is driven by Scer\GAL4^elav-C155 can be partially suppressed by simultaneous expression of beat-Ia^Scer\UAS.cFa.

(Landgraf et al., 1999)

Ectopic expression of eve^Scer\UAS.cBa under the control of Scer\GAL4^twi.PG in a zfh1² background restores the formation of some eve-expressing pericardial cells.

(Su et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Phenotype dependent on Scer\GAL4.

(Brand and Perrimon, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

eve^Scer\UAS.cBa

eve^UAS.cBa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Brand

Secondary FlyBase IDs

References (19)

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