FB2024_03 , released June 25, 2024
Allele: Dmel\beat-IaUAS.cFa
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General Information
Symbol
Dmel\beat-IaUAS.cFa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Fambrough
FlyBase ID
FBal0057746
Feature type
allele
Associated gene
Dmel\beat-Ia
Associated Insertion(s)
Carried in Construct
P{UAS-beat.F}
Also Known As
UAS-beat
Key Links
Transgenic product class
cDNA
(Fambrough and Goodman, 1996)
wild_type
(Fambrough and Goodman, 1996)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Fambrough and Goodman, 1996)
wild_type
(Fambrough and Goodman, 1996)
Mutagen
in vitro construct
(Heilig, 1998.10.3, Fambrough and Goodman, 1996)
Progenitor genotype
Carried in construct
P{UAS-beat.F}
Cytology
Description

UASt regulatory sequences drive expression of a beat-Ia cDNA.

(Fambrough and Goodman, 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
beat-Ia
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of beat-IaScer\UAS.cFa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to both aCC and RP2 motor neurons displaying an almost exclusively single exit from the CNS.

      Misexpression of beat-IaScer\UAS.cFa in EW neurons under the control of Scer\GAL4eg-Mz360 does not alter axonal projection.

      (Zarin et al., 2014)

      Third instar larvae expressing beat-IaScer\UAS.cFa under the control of Scer\GAL4Mef2.PR sometimes lack neuromuscular junctions (NMJs) on dorsal muscles (the percentage of muscles lacking NMJs is given in parentheses); muscle 1 (16%), muscle 9 (21%), muscle 2 (7%), muscle 10 (5%).

      (Siebert et al., 2009)

      Larval visual system development occurs normally in 93% of beat-Iatric-1 embryos expressing beat-IaScer\UAS.cFa under the control of Scer\GAL4bi-omb-Gal4. However, a large number of these embryos fail to develop.

      (Holmes and Heilig, 1999)

      Scer\GAL4how-24B mediated expression causes major motoneuron outgrowth defects.

      (Fambrough and Goodman, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Enhancer of
      Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of beat-IaScer\UAS.cFa in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment. These mutants exhibit an increase in dorsal projections in 36% of hemisegments.

      Co-expression of beat-IaScer\UAS.cFa and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of beat-IaScer\UAS.cFa and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of beat-IaScer\UAS.cFa and unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Nrg180.I.Scer\UAS in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Triple misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, and Nrg180.I.Scer\UAS in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 20% of hemisegments.

      Quadrupal misexpression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa, Nrg180.I.Scer\UAS and Fas2Scer\UAS.cLa in EW neurons, under the control of Scer\GAL4eg-Mz360, leads to lateral redirection of axons in 33% of hemisegments.

      (Zarin et al., 2014)

      Co-expression of beat-IaScer\UAS.cFa suppresses the lack of neuromuscular junctions seen on dorsal muscles which is caused by expression of sideScer\UAS.cSa under the control of Scer\GAL4Mef2.PR.

      (Siebert et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      beat-IaUAS.cFa/Scer\GAL4elav.PLu rescues beat-IaC163/beat-Ia3

      (Siebert et al., 2009)

      beat-IaUAS.cFa/Scer\GAL4Fas2-Mz507 rescues beat-IaC163/beat-Ia3

      (Siebert et al., 2009)

      Scer\GAL4Kr.PM/beat-IaUAS.cFa rescues beat-Iatric-1

      (Holmes and Heilig, 1999)

      beat-IaUAS.cFa/Scer\GAL4GMR.PF rescues beat-Iatric-1

      (Holmes and Heilig, 1999)

      beat-IaUAS.cFa/Scer\GAL4unspecified rescues beat-Iatric-1

      (Heilig, 1998.10.3)

      beat-IaUAS.cFa/Scer\GAL4unspecified rescues beat-Iaunspecified

      (Heilig, 1998.10.3)

      Scer\GAL4elav-C155/beat-IaUAS.cFa rescues beat-Ia2

      (Fambrough and Goodman, 1996)

      Scer\GAL4elav-C155/beat-IaUAS.cFa rescues beat-Ia3

      (Fambrough and Goodman, 1996)
      Partially rescues

      beat-IaUAS.cFa/Scer\GAL4bi-omb-Gal4 partially rescues beat-Iatric-1

      (Holmes and Heilig, 1999)
      Fails to rescue

      beat-IaUAS.cFa/Scer\GAL4Mef2.PR fails to rescue beat-IaC163/beat-Ia3

      (Siebert et al., 2009)
      Comments

      The neuromuscular junction innervation defects seen in the dorsal muscles of beat-Ia3/beat-IaC163 third instar larvae are rescued by expression of beat-IaScer\UAS.cFa under the control of Scer\GAL4elav.PLu or Scer\GAL4Fas2-Mz507, but are not rescued by expression of beat-IaScer\UAS.cFa under the control of Scer\GAL4Mef2.PR.

      (Siebert et al., 2009)

      Rescues the Bolwig organ phenotype of beat-Iatric-1 and beat-Iaunspecified mutants when expression is directed to the Bolwig organs or optic lobes.

      (Heilig, 1998.10.3)

      Scer\GAL4elav-C155 mediated expression of beat-IaScer\UAS.cFa rescues the SNb divergence defect of beat-Ia2/beat-Ia3 transheterozygotes.

      (Fambrough and Goodman, 1996)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (5)
      Reported As
      Symbol Synonym
      beat-IaScer\UAS.cFa
      beat-IaUAS.cFa
      beatScer\UAS.cFa
      beatUAS.cFa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Fambrough
      (Fambrough and Goodman, 1996)
      Secondary FlyBase IDs
        References (7)