FB2024_03 , released June 25, 2024
Allele: Dmel\Fas2UAS.cLa
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General Information
Symbol
Dmel\Fas2UAS.cLa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Lin
FlyBase ID
FBal0044814
Feature type
allele
Associated gene
Dmel\Fas2
Associated Insertion(s)
Carried in Construct
P{UAS-Fas2.PEST-}
Also Known As
UAS-FasII, UAS-Fas2, UAS-fasciclinII, UAS-Fas II
Key Links
Transgenic product class
cDNA
(Lin and Goodman, 1994)
wild_type
(Lin and Goodman, 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Lin and Goodman, 1994)
wild_type
(Lin and Goodman, 1994)
Mutagen
in vitro construct
(Lin and Goodman, 1994)
Progenitor genotype
Carried in construct
P{UAS-Fas2.PEST-}
Cytology
Description

Expression of the Fas2 PEST cDNA is governed by UAS regulatory sequences.

(Lin and Goodman, 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Fas2
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal ventral muscle cell | embryonic stage, with Scer\GAL4how-24B

      (Nose et al., 1997)

      adult mushroom body, with Scer\GAL4238Y

      (Kurusu et al., 2002)

      adult mushroom body, with Scer\GAL4elav-C155

      (Kurusu et al., 2002)

      adult mushroom body, with Scer\GAL4ey-OK107

      (Kurusu et al., 2002)

      adult mushroom body alpha'-lobe, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      adult mushroom body alpha'-lobe, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      adult mushroom body alpha'-lobe | pupal stage, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      adult mushroom body alpha-lobe, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      adult mushroom body alpha-lobe, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      adult mushroom body alpha-lobe | pupal stage, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      adult mushroom body beta'-lobe, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      adult mushroom body beta-lobe, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      adult mushroom body beta-lobe, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      adult mushroom body gamma-lobe, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      adult neuromuscular junction, with Scer\GAL4elav-C155

      (Hebbar and Fernandes, 2005)

      adult neuromuscular junction, with Scer\GAL4Mhc.PW

      (Hebbar and Fernandes, 2005)

      axon, with Scer\GAL4Tab2-201Y

      (Bornstein et al., 2015)

      axon & eye photoreceptor cell, with Scer\GAL4GMR.PF

      (Cafferty et al., 2006)

      axon & lamina, with Scer\GAL4GMR.PF

      (Cafferty et al., 2006)

      border follicle cell, with Scer\GAL4BA3

      (Szafranski and Goode, 2004)

      eye photoreceptor cell & lamina, with Scer\GAL4GMR.PF

      (Cafferty et al., 2006)

      indirect flight muscle motor neuron DLMN5, with Scer\GAL4elav-C155

      (Hebbar and Fernandes, 2005)

      indirect flight muscle motor neuron DLMN5, with Scer\GAL4Mhc.PW

      (Hebbar and Fernandes, 2005)

      larval abdominal intersegmental nerve, with Scer\GAL4C38

      (Lin and Goodman, 1994)

      larval abdominal intersegmental nerve, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      larval abdominal segmental nerve, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      larval abdominal segmental nerve | embryonic stage, with Scer\GAL4how-24B

      (Nose et al., 1997)

      larval DA1 motor neuron, with Scer\GAL4eve.RN2

      (Zarin et al., 2014)

      larval intersegmental nerve branch ISNb of A1-7, with Scer\GAL4elav.PU

      (Miller et al., 2008)

      larval MP1 neuron, with Scer\GAL4ftz.ng

      (Lin et al., 1994)

      larval RP2 motor neuron, with Scer\GAL4eve.RN2

      (Zarin et al., 2014)

      lobe system of adult mushroom body, with Scer\GAL4c305a

      (Kucherenko et al., 2012)

      macrochaeta, with Scer\GAL4A111

      (Garcia-Alonso et al., 1995)

      macrochaeta, with Scer\GAL4D45

      (Garcia-Alonso et al., 1995)

      macrochaeta, with Scer\GAL4l(3)C109-C109

      (Garcia-Alonso et al., 1995)

      medial lobe of adult mushroom body, with Scer\GAL4238Y

      (Kurusu et al., 2002)

      medial lobe of adult mushroom body, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      medial lobe of adult mushroom body, with Scer\GAL4elav-C155

      (Kurusu et al., 2002)

      NMJ bouton | third instar larval stage, with Scer\GAL4RapGAP1-OK6

      (Beck et al., 2012)

      pedunculus of adult mushroom body, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      pedunculus of adult mushroom body, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      pedunculus of larval mushroom body, with Scer\GAL4c739

      (Fushima and Tsujimura, 2007)

      pedunculus of larval mushroom body, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      synapse & abdominal 2 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      synapse & abdominal 3 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      synapse & abdominal 4 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      synapse & abdominal 5 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      synapse & abdominal 6 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      synapse & abdominal 7 ventral longitudinal muscle 1, with Scer\GAL4elav-C155

      (Lin and Goodman, 1994)

      vertical lobe of adult mushroom body, with Scer\GAL4elav-C155

      (Kurusu et al., 2002)

      vertical lobe of larval mushroom body, with Scer\GAL4ey-OK107

      (Fushima and Tsujimura, 2007)

      vMP2 neuron, with Scer\GAL4ftz.ng

      (Lin et al., 1994)
      Detailed Description
      Statement
      Reference

      Overexpression of Fas2Scer\UAS.cLa by Scer\GAL4Tab2-201Y results in significant axon (but not dendrite) pruning defects.

      (Bornstein et al., 2015)

      Expression of Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to both aCC and RP2 motor neurons displaying an almost exclusively single exit from the CNS.

      (Zarin et al., 2014)

      Scer\GAL4Rapgap1-OK6-mediated expression of Fas2Scer\UAS.cLa induces a significant increase in NMJ synaptic bouton number.

      (Beck et al., 2012)

      Expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4let-7-C does not alter mushroom body α/β lobe morphology.

      Expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4c305a results in α'/β' mushroom body lobe neurons projecting into the α/β lobe.

      (Kucherenko et al., 2012)

      Scer\GAL4elav.PU Fas2Scer\UAS.cLa embryos display a high degree of ISNb hyperfasciculation.

      (Miller et al., 2008)

      Expression of Fas2UAS.cLa under the control of Scer\GAL4c739 results in defects in mushroom body lobes in some adults; this can include lobes extending in the wrong direction, thicker lobes, thinner lobes, missing lobes and ectopic masses of axons at the tip of the peduncle, none of these phenotypes occur in control animals. Expression of Fas2UAS.cLa under the control of Scer\GAL4c739 does not affect mushroom body lobe structure in the larva, but can cause defects in the peduncle; during early metamorphosis, some alpha' lobes collapse, this is correlated with misdirection of the alpha lobe and does not occur in controls. Expression of Fas2UAS.cLa under the control of Scer\GAL4Tab2-201Y does not lead to mushroom body lobe defects in the larva or the adult. Expression of Fas2UAS.cLa under the control of Scer\GAL4NP2082 does not lead to mushroom body lobe defects in the larva or the adult. Expression of Fas2UAS.cLa under the control of Scer\GAL4ey-OK107 leads to a lack of extension of any mushroom body lobes (including gamma, alpha', beta', alpha and beta) in any direction from the peduncle and the formation of a mass of axons at the tip of the peduncle in the adult brain. In the larva, expression of Fas2UAS.cLa under the control of Scer\GAL4ey-OK107 sometimes results in a thinner dorsal lobe or a shift of the core of the peduncle closer to the border of the peduncle.

      (Fushima and Tsujimura, 2007)

      In late third instar Fas2Scer\UAS.cLa; Scer\GAL4GMR.PF larvae, the axons of eye photoreceptor cells are hyperfasciculated.

      (Cafferty et al., 2006)

      The number of secondary branches on the motor neuron that innervates DLMa is reduced when Fas2Scer\UAS.cLa is expressed under the control of the neuronal Scer\GAL4elav-C155 driver. As a result, the pool of secondary branches that can form higher order branches is reduced and the number of contact points between the neuron and the muscle is significantly reduced (from 5 to 4.3). When Fas2Scer\UAS.cLa is expressed in muscle, under the control of Scer\GAL4Mhc.PW, there is a reduction in the amount of secondary branches on the motor neuron that innervates DLMa. However, secondary branches are longer and occupy a greater area on the muscle surface than wild-type branches. The lower numbers of secondary branches results in a lower number of contact points between the neuron and the muscle (from 5 to 4.4) and a reduction in overall muscle length.

      (Hebbar and Fernandes, 2005)

      In Fas2Scer\UAS.cLa; Scer\GAL4BA3 flies the border follicle cells have much reduced polarity. Delamination of these cells is delayed (they initiate migration behind the trailing edge of the epithelium) but their migration rate is normal.

      (Szafranski and Goode, 2004)

      When Fas2Scer\UAS.cLa is driven by Scer\GAL4elav-C155, severe morphological abnormalities are seen in the third instar mushroom bodies. Both the dorsal and medial lobes are markedly affected. The core is also disrupted. When Fas2Scer\UAS.cLa is driven by Scer\GAL4OK107, developmental defects are seen in the majority of mushroom bodies. When Fas2Scer\UAS.cLa is driven by Scer\GAL4Tab2-201Y, no mushroom body defects are seen. When Fas2Scer\UAS.cLa is driven by Scer\GAL4238Y, only mild defects are seen in mushroom bodies. Medial lobes terminate in single blob but internally harbour three branches.

      (Kurusu et al., 2002)

      Embryos expressing Fas2Scer\UAS.cLa under the control of Scer\GAL4elav-C155 show modest hyperfasciculation defects in the intersegmental nerve b (ISNb).

      (Yu et al., 2000)

      Scer\GAL4how-24B-mediated expression promotes muscle-muscle adhesion in vivo at a high level. Extensive muscle-muscle adhesion between subsets of ventral muscles causes defects in innervation, SNb axons reach the target region normally but the terminal abors on the internal muscle surface are greatly reduced. No abnormality in SNb axon trajectory is seen, they enter the ventral muscle field at the correct choice point and extend toward the target region normally.

      (Nose et al., 1997)

      Extra bristles in the vicinity of the postvertical bristles and other regions of the adult fly.

      (Garcia-Alonso et al., 1995)

      In a P{GawB}elavC155 background the SNb axons are dramatically affected, showing bypass, stall and detour phenotypes. SNa axons are also affected, showing predominantly stall mutant phenotypes. The ISN also shows a misrouting phenotype. Axons extend from below muscle 12 to synapse on the outside, dorsal surface of the fiber: the "reach-back" phenotype In a P{GawB}C38 background causes extensive ISN growth cone exploration of and adhesive contact to the tracheal cells which is never observed in wild type. The ISN can extend past the main tracheal trunk and the contacts are withdrawn in late stage 16.

      (Lin and Goodman, 1994)

      Scer\GAL4ftz.ng expression causes a novel gain of function FN3/MP1 fused pathway phenotype at stage 16 (fused phenotype is not continuous but rather occurs for short stretches) and fused vMP2 and MP1 pathways at stage 14.

      (Lin et al., 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhanced by
      Suppressed by
      Enhancer of
      NOT Suppressor of
      Other
      Phenotype Manifest In
      Enhanced by
      Statement
      Reference

      Fas2UAS.cLa, Scer\GAL4Tab2-201Y has axon phenotype, enhanceable by bskDN.UAS.cUa

      (Bornstein et al., 2015)
      Suppressed by
      Enhancer of
      Statement
      Reference

      Scer\GAL4eve.RN2/Fas2UAS.cLa is an enhancer of larval VUM motor neuron phenotype of eveΔRP2A

      (Zarin et al., 2014)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Df(2R)eve, Scer\GAL4eve.RN2, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      unc-5UAS.Tag:HA,Tag:SS(wg), Fas2UAS.cLa, Df(2R)eve, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Scer\GAL4eve.RN2, beat-IaUAS.cFa

      (Zarin et al., 2014)

      Fas2UAS.cLa, Scer\GAL4eg-Mz360 is an enhancer of larval EW neuron phenotype of Nrg180.I.UAS, Scer\GAL4eg-Mz360, beat-IaUAS.cFa, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      Nrg180.I.UAS, beat-IaUAS.cFa, Fas2UAS.cLa, Scer\GAL4eg-Mz360 is an enhancer of larval EW neuron phenotype of Scer\GAL4eg-Mz360, unc-5UAS.Tag:HA,Tag:SS(wg)

      (Zarin et al., 2014)

      Fas2UAS.cLa/Df(2R)eve, Scer\GAL4eve.RN2 is an enhancer of larval VUM motor neuron phenotype of Scer\GAL4eve.RN2, beat-IaUAS.cFa

      (Zarin et al., 2014)

      Fas2UAS.cLa is an enhancer of axon & eye photoreceptor cell phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa is an enhancer of axon & lamina phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa is an enhancer of eye photoreceptor cell & lamina phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa is an enhancer of axon & medulla phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa is an enhancer of eye photoreceptor cell & medulla phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa is an enhancer of nerve terminal & lamina plexus phenotype of Scer\GAL4GMR.PF, Sema1aUAS.cYa

      (Cafferty et al., 2006)

      Fas2UAS.cLa, eve2xCQ.RC.G4DBD is an enhancer of larval prothoracic intersegmental nerve phenotype of RdlUAS.cSa, eve2xCQ.RC.G4DBD

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval intersegmental nerve phenotype of RdlUAS.cSa, Scer\GAL4eve.RN2

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval abdominal intersegmental nerve phenotype of RdlUAS.cSa, Scer\GAL4eve.RN2

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval mesothoracic intersegmental nerve phenotype of RdlUAS.cSa, Scer\GAL4eve.RN2

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval metathoracic intersegmental nerve phenotype of RdlUAS.cSa, Scer\GAL4eve.RN2

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, Scer\GAL4eve.RN2 is an enhancer of larval prothoracic intersegmental nerve phenotype of RdlUAS.cSa, Scer\GAL4eve.RN2

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, eve2xCQ.RC.G4DBD is an enhancer of larval intersegmental nerve phenotype of RdlUAS.cSa, eve2xCQ.RC.G4DBD

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, eve2xCQ.RC.G4DBD is an enhancer of larval abdominal intersegmental nerve phenotype of RdlUAS.cSa, eve2xCQ.RC.G4DBD

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, eve2xCQ.RC.G4DBD is an enhancer of larval mesothoracic intersegmental nerve phenotype of RdlUAS.cSa, eve2xCQ.RC.G4DBD

      (Sanchez-Soriano and Prokop, 2005)

      Fas2UAS.cLa, eve2xCQ.RC.G4DBD is an enhancer of larval metathoracic intersegmental nerve phenotype of RdlUAS.cSa, eve2xCQ.RC.G4DBD

      (Sanchez-Soriano and Prokop, 2005)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of larval VO2 motor neuron phenotype of Sema1ak13702

      (Yu et al., 2000)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of RP3 neuron & synapse phenotype of Sema1ak13702

      (Yu et al., 2000)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of larval VO1 motor neuron phenotype of Sema1ak13702

      (Yu et al., 2000)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of larval RP5 motor neuron phenotype of Sema1ak13702

      (Yu et al., 2000)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of larval abdominal segmental nerve phenotype of Sema1ak13702

      (Yu et al., 2000)

      Scer\GAL4elav-C155/Fas2UAS.cLa is an enhancer of larval intersegmental nerve phenotype of Sema1ak13702

      (Yu et al., 2000)
      NOT Suppressor of
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Fas2Scer\UAS.cLa, Fas2Scer\UAS.cLb or Fas2C.Scer\UAS in third instar larvae with GluRIICdsRNA.Scer\UAS.cBa simultaneously driven by Scer\GAL4C57 and Scer\GAL4elav-C155 does not impair homeostatic compensation at the neuromuscular junction (significant reduction in mEPSP and significant increase in quantal content compared to controls, just as is seen in larvae with expression of GluRIICdsRNA.Scer\UAS.cBa simultaneously driven by Scer\GAL4C57 and Scer\GAL4elav-C155).

      (Spring et al., 2016)

      Co-expression of bskDN.Scer\UAS.cUa enhances axon-pruning defects in flies with Fas2Scer\UAS.cLa driven by Scer\GAL4Tab2-201Y.

      (Bornstein et al., 2015)

      Expression of Fas2Scer\UAS.cLa in an eveΔRP2A mutant background results in the exit of a single motoneuron of the pair in each hemisegment. These mutants exhibit an increase in dorsal projections in 25% of hemisegments.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      Co-expression of unc-5Scer\UAS.T:Ivir\HA1,T:SS-wg, beat-IaScer\UAS.cFa and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. The number of hemisegments with one neuronal exit is reduced, compared to controls and single and double mutants.

      Co-expression of beat-IaScer\UAS.cFa and Fas2Scer\UAS.cLa in a Df(2R)eve mutant background, under the control of Scer\GAL4eve.RN2 leads to a more robust CNS exit for motor neurons, with many hemisegments displaying exit of both motor neurons. There are two types of exit: unfasciculated, both axons from the same hemisegment chose a different nerve root, or fasciculated, where axons join and exit through the intersegmental nerve root.

      (Zarin et al., 2014)

      Expression of Fas2Scer\UAS.cLa fails to suppress the synaptic retraction phenotype seen in the NMJs of third instar larvae when NrgGD82 is expressed under the control of Scer\GAL4elav-C155 and Scer\GAL4VGlut-OK371.

      (Enneking et al., 2013)

      Scer\GAL4Rapgap1-OK6-mediated expression of Fas2Scer\UAS.cLa does not rescue synaptic bouton number or average synaptic bouton area of beag1/Df(3R)Exel6151 animals.

      (Beck et al., 2012)

      The hyperfasciculation phenotype seen in the axons of eye photoreceptor cells in ate third instar Sema-1aScer\UAS.cYa; Scer\GAL4GMR.PF larvae is enhanced by Fas2Scer\UAS.cLa - axons are clustered into relatively few large bundles in the lamina and medulla.

      (Cafferty et al., 2006)

      Expression of Fas2Scer\UAS.cLa pre- and post-synaptically, under the control of Scer\GAL4A307 in a Nrg849 heterozygous background fails to suppresses the Nrg849 giant synapse physiological phenotype.

      (Godenschwege et al., 2006)

      Coexpression of RdlScer\UAS.cSa and Fas2Scer\UAS.cLa in the 3 U motorneurons (under the control of eve2xCQ.RC.T:Scer\GAL4 results in the co-arrest of 54% of the intersegmental motor nerves, indicating an enhancement on the ISN phenotype observed in RdlScer\UAS.cSa;eve2xCQ.RC.T:Scer\GAL4 overexpression experiments.

      Coexpression of RdlScer\UAS.cSa and Fas2Scer\UAS.cLa in the aCC/RP2 axons (under the control of Scer\GAL4eve.RN2 results in the co-arrest of 97% of the intersegmental motor nerves (ISN), indicating an enhancement on the ISN phenotype observed in RdlScer\UAS.cSa;Scer\GAL4eve.RN2 overexpression experiments (where 70% of ISNs are stalled).

      (Sanchez-Soriano and Prokop, 2005)

      The intersegmental nerve b (ISNb) phenotypes seen in homozygous Sema-1ak13702 embryos are enhanced by Fas2Scer\UAS.cLa expressed under the control of Scer\GAL4elav-C155. The ISNb may fail to defasciculate from the intersegmental nerve (ISN) which results in a fusion bypass with the ISN. ISNb is also seen to stall ventrally on ventral lateral muscles (VLMs) 6-7. The number of hemisegments showing aberrant or absent RP3 innervation of VLMs 6 and 7 is increased. A failure of RP1, RP4 and RP5 to defasciculate around VLMs 13 and 6, a "stall" phenotype, is also increased. Sema-1ak13702 SNa phenotypes are dramatically enhanced by Fas2Scer\UAS.cLa expressed under the control of Scer\GAL4elav-C155. Failure of all SNa lateral branches to defasciculate from the SNa pathway, resulting in the lack of SNa lateral branches, is seen. An increase in the stall phenotype of the dorsal SNa branch is also seen.

      (Yu et al., 2000)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4RapGAP1-OK6/Fas2UAS.cLa rescues Df(1)BSC869/Fas2e76

      (Beck et al., 2012)

      Fas2UAS.cLa/Scer\GAL4BA3 rescues Fas2rd1

      (Szafranski and Goode, 2004)

      Fas2UAS.cLa/Scer\GAL4hs.PB rescues Fas2spin

      (Adam et al., 2003)

      Fas2UAS.cLa/Scer\GAL4Fas2-spin rescues Fas2spin

      (Adam et al., 2003)

      Fas2UAS.cLa/Scer\GAL4Kurs21 rescues Fas2spinR5

      (Adam et al., 2003)

      Fas2UAS.cLa/Scer\GAL4elav.PLu rescues Fas2spinR5

      (Adam et al., 2003)

      Fas2UAS.cLa/Scer\GAL4elav.PLu rescues Fas2EB112

      (Schuster et al., 1996)

      Fas2UAS.cLa/Scer\GAL4Mhc.PW rescues Fas2EB112

      (Schuster et al., 1996)

      Fas2UAS.cLa/Scer\GAL4E62-2 rescues Fas2e76

      (Schuster et al., 1996)

      Fas2UAS.cLa/Scer\GAL4E62-2 rescues Fas2e86

      (Schuster et al., 1996)

      Fas2UAS.cLa/Scer\GAL4unspecified rescues Fas2EB112

      (Garcia-Alonso et al., 1995)
      Partially rescues

      Fas2UAS.cLa/Scer\GAL4Mhc.PW partially rescues Fas2e76

      (Hebbar and Fernandes, 2005)

      Scer\GAL4elav-C155/Fas2UAS.cLa partially rescues Fas2e76

      (Hebbar and Fernandes, 2005)

      Fas2UAS.cLa/Scer\GAL4slbo.2.6 partially rescues Fas2rd1

      (Szafranski and Goode, 2004)

      Fas2UAS.cLa/Scer\GAL4Feb78 partially rescues Fas2spinR5

      (Adam et al., 2003)
      Fails to rescue

      Fas2UAS.cLa/Scer\GAL4Toll-6-D42 fails to rescue Fas2e76

      (Hebbar and Fernandes, 2005)

      Scer\GAL4Aug21/Fas2UAS.cLa fails to rescue Fas2spinR5

      (Adam et al., 2003)

      Scer\GAL4Feb211/Fas2UAS.cLa fails to rescue Fas2spinR5

      (Adam et al., 2003)
      Comments

      Scer\GAL4Rapgap1-OK6-mediated expression of Fas2Scer\UAS.cLa fully rescues the reduced bouton number of Fas2e76/Df(1)BSC869 animals.

      (Beck et al., 2012)

      Expression of Fas2Scer\UAS.cLa can rescue the DLMa innervation phenotype of Fas2e76 mutants, depending on the driver used. Under the control of Scer\GAL4elav-C155, Fas2Scer\UAS.cLa reduces the average number of motor neuron contact points along the DLMa in Fas2e76 mutants from 5.8 to 5.1, while this number is reduced to 4.6 when Fas2Scer\UAS.cLa is driven by Scer\GAL4Mhc.PW.

      (Hebbar and Fernandes, 2005)

      The migration phenotypes of border follicle cells in Fas2rd1 homozygotes are rescued by Fas2Scer\UAS.cLa; Scer\GAL4BA3. When Scer\GAL4slbo.2.6 is used instead, the migration rate of the border follicle cells is rescued, but delamination is still delayed, and the border follicle cells lose their polarity.

      (Szafranski and Goode, 2004)

      Expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4hs.PB rescues the genitalia rotation defects seen in Fas2spin males if a single heat shock is given at 7 days of development. If Fas2Scer\UAS.cLa is expressed under the control of Scer\GAL4hs.PB using heat shock applied before or after this period, little rescuing activity of the Fas2spin male genitalia rotation defects is seen. The genitalia rotation defects seen in Fas2spinR5 males are fully rescued by expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4elav.PLu or Scer\GAL4Kurs21. The genitalia rotation defects seen in Fas2spinR5 males are partially rescued by expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4Feb78. The genitalia rotation defects seen in Fas2spinR5 males are not rescued by expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4Feb211 or Scer\GAL4Aug21. Expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4Feb170 either completely rescues or fails to rescue the genitalia rotation defects seen in Fas2spinR5 males (the Scer\GAL4Feb170 allele shows variegated Scer\GAL4 expression).

      (Adam et al., 2003)

      Lethality of Fas2EB112 is rescued by expression of Fas2Scer\UAS.cLa under the control of Scer\GAL4elav.PLu, Scer\GAL4E62-2 or Scer\GAL4Mhc.PW.

      (Schuster et al., 1996)

      Scer\GAL4 induced expression rescues the lethality of Fas2EB112.

      (Garcia-Alonso et al., 1995)
      Images (0)
      Mutant
      Wild-type
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      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Fas2Scer\UAS.cLa
      Fas2UAS.cLa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Lin
      (Lin and Goodman, 1994)
      Secondary FlyBase IDs
        References (30)