FlyBase Allele Report: Dmel\Rdl[UAS.cSa]

General Information

Symbol

Dmel\Rdl^UAS.cSa

Species

D. melanogaster

Name

FlyBase ID

FBal0216768

Feature type

allele

Associated gene

Dmel\Rdl

Associated Insertion(s)

Carried in Construct

P{UAS-Rdl.S}

Also Known As

UAS-Rdl

Key Links

Transgenic product class

wild_type

(Sanchez-Soriano and Prokop, 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Sanchez-Soriano and Prokop, 2005)

Mutagen

in vitro construct

(Sanchez-Soriano and Prokop, 2005)

Progenitor genotype

Carried in construct

P{UAS-Rdl.S}

Cytology

Description

UASt regulatory sequences drive expression of Rdl coding sequences.

(Sanchez-Soriano and Prokop, 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Rdl

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

abnormal neuroanatomy, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

abnormal neuroanatomy, with Scer\GAL4^Vsx1-MzVum

(Sanchez-Soriano and Prokop, 2005)

Phenotype Manifest In

larval abdominal intersegmental nerve, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval abdominal intersegmental nerve, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval DA1 motor neuron, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval intersegmental nerve, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval intersegmental nerve, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval mesothoracic intersegmental nerve, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval mesothoracic intersegmental nerve, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval metathoracic intersegmental nerve, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval metathoracic intersegmental nerve, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval prothoracic intersegmental nerve, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval prothoracic intersegmental nerve, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval RP2 motor neuron, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval VUM neuron, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

larval VUM neuron, with Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

larval VUM neuron, with Scer\GAL4^Vsx1-MzVum

(Sanchez-Soriano and Prokop, 2005)

U neuron, with eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Detailed Description

Statement

Reference

Expression of Rdl^Scer\UAS.cSa in the aCC/RP2 axons (under the control of Scer\GAL4^eve.RN2), results in stalling, mostly in the dorsolateral muscle field before reaching their most dorsal target muscles (in 70% of hemisegments). Stalled aCC/RP2 axons cause a co-arrest of the complete intersegmental motor neurons, including the VUMs, in 70% of cases. Of the 30% of cases in which aCC/RP2 axons stall but U axons escape, 27% show co-arrest of VUM neurons with aCC/RP2 and 73% show escape.

Expression of Rdl^Scer\UAS.cSa in the aCC/RP2 axons at late larval stages (under the control of Scer\GAL4^eve.RRK), does not affect their morphology.

Expression of Rdl^Scer\UAS.cSa in the U motorneurons (under the control of eve^{2xCQ.RC.T:Scer\GAL4}), results in stalling. Stalled U motorneurons cause a co-arrest of the complete intersegmental motor neurons in 10% of cases. If U neurons are stalled, aCC/RP2 axons escape in 90% of cases. An estimated 4.4% of VUM neurons co-arrest.

Expression of Rdl^Scer\UAS.cSa in the VUM neurons (under the control of Scer\GAL4^MzVum), results in stalling. Stalled VUM axons cause a co-arrest of the complete intersegmental motor neurons in 0% of cases.

(Sanchez-Soriano and Prokop, 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has abnormal neuroanatomy phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has abnormal neuroanatomy phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Suppressed by

Statement

Reference

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has abnormal neuroanatomy phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Suppressor of

Statement

Reference

Rdl^UAS.cSa, Scer\GAL4^GS.Ilp2.PU is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^GS.Ilp2.PU, wake^GD12087

(Chen et al., 2022)

Rdl^UAS.cSa, Scer\GAL4^GS.Ilp2.PU is a suppressor | partially of abnormal courtship behavior | RU486 conditional phenotype of Scer\GAL4^GS.Ilp2.PU, wake^KK112014

(Chen et al., 2022)

Phenotype Manifest In

Enhanced by

Statement

Reference

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has larval intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has larval abdominal intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval prothoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has larval mesothoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has larval metathoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, eve^{2xCQ.RC.G4DBD} has larval prothoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, eve^{2xCQ.RC.G4DBD}

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval abdominal intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval mesothoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval metathoracic intersegmental nerve phenotype, enhanceable by Fas2^UAS.cLa, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Suppressed by

Statement

Reference

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval intersegmental nerve phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval abdominal intersegmental nerve phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval mesothoracic intersegmental nerve phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval metathoracic intersegmental nerve phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Rdl^UAS.cSa, Scer\GAL4^eve.RN2 has larval prothoracic intersegmental nerve phenotype, suppressible by Fas2^EB112, Scer\GAL4^eve.RN2

(Sanchez-Soriano and Prokop, 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Coexpression of Rdl^Scer\UAS.cSa and Fas2^Scer\UAS.cLa in the 3 U motorneurons (under the control of eve^{2xCQ.RC.T:Scer\GAL4} results in the co-arrest of 54% of the intersegmental motor nerves, indicating an enhancement on the ISN phenotype observed in Rdl^Scer\UAS.cSa;eve^{2xCQ.RC.T:Scer\GAL4} overexpression experiments.

Coexpression of Rdl^Scer\UAS.cSa and Fas2^Scer\UAS.cLa in the aCC/RP2 axons (under the control of Scer\GAL4^eve.RN2 results in the co-arrest of 97% of the intersegmental motor nerves (ISN), indicating an enhancement on the ISN phenotype observed in Rdl^Scer\UAS.cSa;Scer\GAL4^eve.RN2 overexpression experiments (where 70% of ISNs are stalled).

Intersegmental nerves are co-arrested upon expression of Rdl^Scer\UAS.cSa in the aCC/RP2 neurons (under the control of Scer\GAL4^eve.RN2) in a Fas2^EB112 mutant background in 36% of cases, compared with 70% in a wild-type background.

(Sanchez-Soriano and Prokop, 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^GS.Ilp2.PU/Rdl^UAS.cSa partially rescues Rdl^GD4609

(Chen et al., 2022)

Scer\GAL4^GS.Ilp2.PU/Rdl^UAS.cSa partially rescues Rdl^JF01455

(Chen et al., 2022)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

29036

w^* P{UAS-Rdl.S}8.2; sna^Sco/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Rdl^Scer\UAS.cSa

Rdl^UAS.cSa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Sanchez-Soriano

(Sanchez-Soriano and Prokop, 2005)

Secondary FlyBase IDs

References (3)

Report Sections