FlyBase Allele Report: Dmel\wake[GD12087]

General Information

Symbol

Dmel\wake^GD12087

Species

D. melanogaster

Name

FlyBase ID

FBal0205269

Feature type

allele

Associated gene

Dmel\wake

Associated Insertion(s)

Carried in Construct

P{GD12087}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD12087}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD12087

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity, with Scer\GAL4^insc-Mz1407

(Mauri et al., 2014)

abnormal cell polarity, with Scer\GAL4^pnr-MD237

(Mauri et al., 2014)

abnormal courtship behavior, with Scer\GAL4^elav.PU

(Chen et al., 2022)

abnormal courtship behavior, with Scer\GAL4^NP1350

(Chen et al., 2022)

abnormal courtship behavior, with Scer\GAL4^NP3168

(Chen et al., 2022)

abnormal courtship behavior, with Scer\GAL4^NP3624

(Chen et al., 2022)

abnormal courtship behavior, with Scer\GAL4^wake-15185

(Chen et al., 2022)

abnormal courtship behavior | RU486 conditional, with Scer\GAL4^{Act5C.Switch.PR}

(Chen et al., 2022)

abnormal courtship behavior | RU486 conditional, with Scer\GAL4^GS.Ilp2.PU

(Chen et al., 2022)

viable, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

visible, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Phenotype Manifest In

external sensory organ precursor cell IIa, with Scer\GAL4^pnr-MD237

(Mauri et al., 2014)

external sensory organ precursor cell IIb | ectopic, with Scer\GAL4^pnr-MD237

(Mauri et al., 2014)

tormogen cell, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

Detailed Description

Statement

Reference

Expression of wake^GD12087 under the control of Scer\GAL4^pnr-MD237 causes defects in the establishment of polarity in sensory organ precursor (SOP) cells. Expression of wake^GD12087 causes the formation of external sensory organs that contain two neurons and two sheath cells, indicating a cell transformation of pIIa into pIIb.

Expression of wake^GD12087 in all neuroblasts under the control of Scer\GAL4^insc-Mz1407 does not cause an obvious change in neuroblast numbers in the larval brain, however it does produce defects in the basal segregation of mira.

(Mauri et al., 2014)

Adults expressing wake^GD12087 under the control of Scer\GAL4^elav.PLu (in the presence of Dcr-2^Scer\UAS.cDa to increase the efficiency of RNAi) do not show a significant defect in avoidance of noxious temperature (46[o]C) compared to control flies.

(Neely et al., 2010)

Expression under the control of Scer\GAL4^pnr-MD237 results in external sensory organs consisting of empty or multiple sockets in 0% or 10-20% of the Scer\GAL4^pnr-MD237 expression domain of the notum, depending on the insertion line used.

(Mummery-Widmer et al., 2009)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12087

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by InR^DN.UAS.cUa, Scer\GAL4^{Act5C.Switch.PR}

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Pi3K92E^A2860C.UAS, Scer\GAL4^{Act5C.Switch.PR}

(Chen et al., 2022)

Scer\GAL4^{Act5C.Switch.PR}, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Scer\GAL4^{Act5C.Switch.PR}/chico^KK107581

(Chen et al., 2022)

Scer\GAL4^GS.Ilp2.PU, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Df(3L)Ilp2-3/+

(Chen et al., 2022)

Scer\GAL4^GS.Ilp2.PU, wake^GD12087 has abnormal courtship behavior | RU486 conditional phenotype, suppressible | partially by Rdl^UAS.cSa, Scer\GAL4^GS.Ilp2.PU

(Chen et al., 2022)

Other

Statement

Reference

Scer\GAL4^insc-Mz1407, dlg1^GD4689, wake^GD12087 has increased occurrence of cell division phenotype

(Mauri et al., 2014)

Phenotype Manifest In

Other

Statement

Reference

Scer\GAL4^insc-Mz1407, dlg1^GD4689, wake^GD12087 has neuroblast | increased number phenotype

(Mauri et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of wake^GD12087 and dlg1^GD4689 in all neuroblasts under the control of Scer\GAL4^insc-Mz1407 causes massive overproliferation of neuroblasts, accompanied by increased mitotic activity.

(Mauri et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

12087

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

CG45058^GD12087

CG6954^GD12087

wake^GD12087

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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