FlyBase Allele Report: Dmel\Nrg[GD82]

General Information

Symbol

Dmel\Nrg^GD82

Species

D. melanogaster

Name

FlyBase ID

FBal0207787

Feature type

allele

Associated gene

Dmel\Nrg

Associated Insertion(s)

Carried in Construct

P{GD82}

Key Links

Genomic Maps

JBrowse

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

RNAi_reagent

(Dickson et al., 2007.7.18)

Mutagen

in vitro construct

(Dickson et al., 2007.7.18)

Progenitor genotype

Carried in construct

P{GD82}

Cytology

Description

UASt regulatory sequences drive expression of an inverted repeat.

(Dickson et al., 2007.7.18)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

dsRNA-GD82

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage

(Clements et al., 2021)

abnormal neuroanatomy | adult stage, with Scer\GAL4^Ilp2.PR

(Clements et al., 2021)

abnormal neuroanatomy | adult stage, with Scer\GAL4^repo.PL

(Clements et al., 2021)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^elav-C155

(Enneking et al., 2013)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^VGlut-OK371, Scer\GAL4^elav-C155

(Enneking et al., 2013)

abnormal oxidative stress response | adult stage, with Scer\GAL4^Ilp2.PR

(Clements et al., 2021)

abnormal starvation stress response | adult stage, with Scer\GAL4^Ilp2.PR

(Clements et al., 2021)

abnormal starvation stress response | adult stage, with Scer\GAL4^repo.PL

(Clements et al., 2021)

lethal, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

lethal, with Scer\GAL4^Act5C.PU

(Dietzl et al., 2007)

lethal - all die before end of pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

lethal - all die during embryonic stage, with Dcr-2^UAS.cDa, Scer\GAL4^elav.PLu

(Neely et al., 2010)

some die during pupal stage, with Scer\GAL4^pnr-MD237

(Mummery-Widmer et al., 2009)

viable, with Scer\GAL4^Mef2.PR

(Schnorrer et al., 2010)

viable, with Scer\GAL4^tin.CΔ4

(Neely et al., 2010)

Phenotype Manifest In

Detailed Description

Statement

Reference

Presynaptic expression of Nrg^GD82 under the control of Scer\GAL4^elav-C155 results in synaptic retraction in third instar larval NMJs. The frequency of retractions is increased when Nrg^GD82 is co-expressed with Dcr-2^Scer\UAS.cDa, or if two motor neuron drivers are used in combination (Scer\GAL4^elav-C155 and Scer\GAL4^VGlut-OK371).

Muscle-specific expression of Nrg^GD82 under the control of Scer\GAL4^Mef2.PU (co-expressed with Dcr-2^Scer\UAS.cDa) does not produce synaptic retraction in third instar larval NMJs.

(Enneking et al., 2013)

External Data

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Nrg^GD82, Scer\GAL4^VGlut-OK371, Scer\GAL4^elav-C155 has abnormal neuroanatomy | third instar larval stage phenotype, non-suppressible by Fas2^UAS.cLa, Scer\GAL4^VGlut-OK371, Scer\GAL4^elav-C155

(Enneking et al., 2013)

Other

Statement

Reference

Dcr-2^UAS.cDa, Nrg^GD82, Scer\GAL4^pnr-MD237 has lethal phenotype

(Dietzl et al., 2007)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Nrg^GD82, Scer\GAL4^elav-C155 has embryonic/larval neuromuscular junction | third instar larval stage phenotype, non-suppressible by Scer\GAL4^VGlut-OK371/Fas2^UAS.cLa, Scer\GAL4^elav-C155

(Enneking et al., 2013)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Fas2^Scer\UAS.cLa fails to suppress the synaptic retraction phenotype seen in the NMJs of third instar larvae when Nrg^GD82 is expressed under the control of Scer\GAL4^elav-C155 and Scer\GAL4^VGlut-OK371.

(Enneking et al., 2013)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Nrg^GD82 is rescued by Scer\GAL4^elav-C155/Nrg^UAS.180.cEa

(Enneking et al., 2013)

Comments

Expression of Nrg^{Scer\UAS.180.cEa} significantly rescues the synapse retraction phenotype seen when Nrg^GD82 is expressed under the control of Scer\GAL4^elav-C155 and Scer\GAL4^VGlut-OK371.

(Enneking et al., 2013)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Linkouts

Bristle Screen Database (Knoblich Lab) - A database for RNAi phenotypes in bristle and notum development

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

Nrg^GD82

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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