FB2024_03 , released June 25, 2024
Allele: Dmel\NrgUAS.180.cEa
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General Information
Symbol
Dmel\NrgUAS.180.cEa
Species
D. melanogaster
Name
FlyBase ID
FBal0291689
Feature type
allele
Associated gene
Dmel\Nrg
Associated Insertion(s)
Carried in Construct
P{UAS-Nrg.180.E}
Key Links
Transgenic product class
wild_type
(Enneking et al., 2013)
Mutagen
Nature of the Allele
Transgenic product class
wild_type
(Enneking et al., 2013)
Mutagen
in vitro construct
(Enneking et al., 2013)
Progenitor genotype
Carried in construct
P{UAS-Nrg.180.E}
Cytology
Description

UAS regulatory sequences drive expression of the full-length Nrg180 ORF.

(Enneking et al., 2013)
Allele components
Component
Use(s)
Regulatory region(s)
UAS
Encoded product / tool
Nrg
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 1 )
      Disease
      Evidence
      References
      model of  MASA syndrome
      CEA
      (Kakad et al., 2018)
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Phenotype Manifest In
      Suppressor of
      NOT Suppressor of
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Expression of NrgScer\UAS.180.cEa restores presynaptic Nrg levels in CkIIαP1/CkIIαTikR but fails to suppress the impairment in synaptic stability found in CkIIαP1/CkIIαTikR animals.

      (Bulat et al., 2014)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Rescues

      Scer\GAL4elav-C155/NrgUAS.180.cEa rescues NrgGD82

      (Enneking et al., 2013)

      NrgUAS.180.cEa, Nrg180YF, Scer\GAL4shot-OK307 rescues Nrgl4

      (Enneking et al., 2013)

      NrgUAS.180.cEa, Nrg180YA, Scer\GAL4shot-OK307 rescues Nrgl4

      (Enneking et al., 2013)

      NrgUAS.180.cEa, Nrg180ΔFIGQY, Scer\GAL4shot-OK307 rescues Nrgl4

      (Enneking et al., 2013)
      Comments

      Expression of NrgScer\UAS.180.cEa significantly rescues the synapse retraction phenotype seen when NrgGD82 is expressed under the control of Scer\GAL4elav-C155 and Scer\GAL4VGlut-OK371.

      Expression of NrgScer\UAS.180.cEa under the control of Scer\GAL4OK307 significantly rescues the electrophysiological and morphological defects seen at the adult Giant Fibre (GF)-Trochanteral Muscle (TTMn) synapse when Nrg180YF is expressed in a Nrgl4 mutant background. Rescue is also seen when NrgScer\UAS.180.cEa is expressed presynaptically (Scer\GAL4c17) or postsynaptically (Scer\GAL4shakB.lethal.4.1), and also when Scer\GAL4c42.2 is expressed presynaptically only after the connection between the GF and the TTMn has been established.

      Expression of NrgScer\UAS.180.cEa under the control of Scer\GAL4OK307 significantly rescues the electrophysiological and morphological defects seen at the adult Giant Fibre (GF)-Trochanteral Muscle (TTMn) synapse when Nrg180YA is expressed in a Nrgl4 mutant background. Rescue is also seen when NrgScer\UAS.180.cEa is expressed presynaptically (Scer\GAL4c17) or postsynaptically (Scer\GAL4shakB.lethal.4.1), but not when Scer\GAL4c42.2 is expressed presynaptically only after the connection between the GF and the TTMn has been established.

      Expression of NrgScer\UAS.180.cEa under the control of Scer\GAL4OK307 significantly rescues the electrophysiological and morphological defects seen at the adult Giant Fibre (GF)-Trochanteral Muscle (TTMn) synapse when Nrg180ΔFIGQY is expressed in a Nrgl4 mutant background. Rescue is also seen when NrgScer\UAS.180.cEa is expressed presynaptically (Scer\GAL4c17) or postsynaptically (Scer\GAL4shakB.lethal.4.1), but not when Scer\GAL4c42.2 is expressed presynaptically only after the connection between the GF and the TTMn has been established.

      (Enneking et al., 2013)

      Expression of NrgScer\UAS.180.cEa significantly rescues the giant fibre axon guidance defects seen in Nrg849 mutant flies.

      (Kudumala et al., 2013)
      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      Reported As
      Symbol Synonym
      NrgScer\UAS.180.cEa
      NrgUAS.180.cEa
      Name Synonyms
      Secondary FlyBase IDs
        References (4)