FlyBase Allele Report: Dmel\CkIIα[LL05896]

General Information

Symbol

Dmel\CkIIα^LL05896

Species

D. melanogaster

Name

FlyBase ID

FBal0295268

Feature type

allele

Associated gene

Dmel\CkIIα

Associated Insertion(s)

PBac{SAstopDsRed}CkIIα^LL05896

Carried in Construct

Also Known As

Ck2α^P1

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Bulat et al., 2014)

Mutagen

Progenitor genotype

Associated Insertion(s)

PBac{SAstopDsRed}CkIIα^LL05896

Cytology

Description

Allele components

Component

Use(s)

Inserted element

PBac{SAstopDsRed}

gene trap

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:23,099,670..23,099,670 [+]

(Bulat et al., 2014)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during third instar larval stage, with CkIIα^{L39A.F52A.UAS.EGFP}, Scer\GAL4^da.G32

(Bulat et al., 2014)

viable, with CkIIα^{F52A.UAS.EGFP}, Scer\GAL4^da.G32

(Bulat et al., 2014)

viable, with CkIIα^{L39A.UAS.EGFP}, Scer\GAL4^da.G32

(Bulat et al., 2014)

Phenotype Manifest In

neuromuscular junction

(Bulat et al., 2014)

neuromuscular junction (with CkIIα^LL07221)

(Bulat et al., 2014)

neuromuscular junction (with CkIIα^Tik)

(Bulat et al., 2014)

neuromuscular junction (with CkIIα^TikR)

(Bulat et al., 2014)

NMJ bouton

(Bulat et al., 2014)

synapse

(Bulat et al., 2014)

synapse (with CkIIα^LL07221)

(Bulat et al., 2014)

synapse (with CkIIα^Tik)

(Bulat et al., 2014)

synapse (with CkIIα^TikR)

(Bulat et al., 2014)

synaptic vesicle

(Bulat et al., 2014)

Detailed Description

Statement

Reference

CkIIα^P1 homozygotes exhibit synaptic retraction in 50% of neuromuscular junctions in muscles 1/9 and 2/10.

CkIIα^P1/CkIIα^P2 transheterozygotes exhibit synaptic retraction in ~35% of neuromuscular junctions in muscles 1/9 and 2/10.

CkIIα^P1/CkIIα^Tik transheterozygotes exhibit synaptic retraction in ~40% of neuromuscular junctions in muscles 1/9 and 2/10.

CkIIα^P1/CkIIα^TikR transheterozygotes exhibit synaptic retraction in ~35% of neuromuscular junctions in muscles 1/9 and 2/10.

CkIIα^P1 homozygotes display a 30% reduction in synaptic bouton number. In addition, synaptic vesicle domains that are clearly separated within boutons in control animals appear fused in CkIIα^P1 mutant animals. The reduction in synaptic vesicle cluster number per neuromuscular junction is still significant even when taking into account the concomitant reduction in neuromuscular junction size.

(Bulat et al., 2014)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

CkIIα^LL05896 has synapse phenotype, enhanceable by Ank2^f02001

(Bulat et al., 2014)

CkIIα^LL05896 has neuromuscular junction phenotype, enhanceable by Ank2^f02001

(Bulat et al., 2014)

NOT suppressed by

Statement

Reference

CkIIα^TikR/CkIIα^LL05896 has synapse phenotype, non-suppressible by Scer\GAL4^elav-C155/Nrg^UAS.180.cEa

(Bulat et al., 2014)

CkIIα^TikR/CkIIα^LL05896 has neuromuscular junction phenotype, non-suppressible by Scer\GAL4^elav-C155/Nrg^UAS.180.cEa

(Bulat et al., 2014)

Enhancer of

Statement

Reference

CkIIα^LL05896 is an enhancer of synapse phenotype of Ank2^f02001

(Bulat et al., 2014)

CkIIα^LL05896 is an enhancer of neuromuscular junction phenotype of Ank2^f02001

(Bulat et al., 2014)

Additional Comments

Genetic Interactions

Statement

Reference

Removal of one copy of CkIIα^P1 in a Ank2^f02001 homozygous background significantly increases the frequency of synaptic retractions.

Removal of one copy of Ank2^f02001 in a CkIIα^P1 homozygous background significantly increases the frequency of synaptic retractions.

Expression of Nrg^{Scer\UAS.180.cEa} restores presynaptic Nrg levels in CkIIα^P1/CkIIα^TikR but fails to suppress the impairment in synaptic stability found in CkIIα^P1/CkIIα^TikR animals.

(Bulat et al., 2014)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

CkIIα^LL05896 is rescued by Scer\GAL4^elav-C155/CkIIα^UAS.N.Tag:HA

(Bulat et al., 2014)

CkIIα^LL05896 is rescued by Scer\GAL4^Mef2.PU/CkIIα^UAS.N.Tag:HA

(Bulat et al., 2014)

CkIIα^LL05896 is rescued by Scer\GAL4^elav-C155/CkIIα^UAS.N.EGFP

(Bulat et al., 2014)

CkIIα^LL05896 is rescued by Scer\GAL4^da.G32/CkIIα^{L39A.UAS.EGFP}

(Bulat et al., 2014)

CkIIα^LL05896 is rescued by CkIIα^{F52A.UAS.EGFP}/Scer\GAL4^da.G32

(Bulat et al., 2014)

Partially rescued by

CkIIα^LL05896 is partially rescued by CkIIα^{L39A.F52A.UAS.EGFP}/Scer\GAL4^da.G32

(Bulat et al., 2014)

Not rescued by

CkIIα^LL05896 is not rescued by Scer\GAL4^da.G32/CkIIα^UAS.N.Tag:HA

(Bulat et al., 2014)

CkIIα^LL05896 is not rescued by Scer\GAL4^elav-C155/CkIIα^{K66M.UAS.N.Tag:HA}

(Bulat et al., 2014)

CkIIα^LL05896 is not rescued by Scer\GAL4^da.G32/CkIIα^{K66M.UAS.N.Tag:HA}

(Bulat et al., 2014)

Comments

Neuronal expression of CkIIα^{Scer\UAS.N.T:Ivir\HA1}, under the control of Scer\GAL4^elav-C155 rescues the neuromuscular junction synaptic retraction phenotype found in CkIIα^P1 homozygotes, while expression post-synaptically, under the control of Scer\GAL4^VGlut-OK371, fails to suppress this phenotype.

Presynaptic expression of wild-type CkIIα^{Scer\UAS.N.T:Ivir\HA1} but not kinase-dead CkIIα^{K66M.Scer\UAS.N.T:Ivir\HA1} is sufficient to rescue synaptic bouton number and vesicle organization.

(Bulat et al., 2014)

Images (0)

Mutant

Wild-type

Stocks (1)

Kyoto

141869

y^* w^*; PBac{SAstopDsRed}CkIIα^LL05896 P{FRT(w^hs)}2A P{neoFRT}82B P{Car20y}96E/TM6B, Tb¹

Notes on Origin

Discoverer

Comments

Based on heterozygous lethality, these alleles of CkIIα can be placed in the following order, from severe to weak: CkIIα^P2 = CkIIα^TikR > CkIIα^P1 > CkIIα^Tik > CkIIα^H3091 > CkIIα^G703.

(Bulat et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Ck2α^P1

(Hirschhäuser et al., 2021)

CkIIα^LL05896

CkIIα^P1

Name Synonyms

Secondary FlyBase IDs

References (2)

Report Sections

Open Close