FlyBase Allele Report: Dmel\CkIIα[Tik]

General Information

Symbol

Dmel\CkIIα^Tik

Species

D. melanogaster

Name

Timekeeper

FlyBase ID

FBal0141857

Feature type

allele

Associated gene

Dmel\CkIIα

Associated Insertion(s)

Carried in Construct

Also Known As

CK2α^Tik, Tik

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lin et al., 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: E165?.

(Bulat et al., 2014)

Amino acid replacement: M161K.

(Bulat et al., 2014)

CkIIα^Tik contains two coding mutations within the presumptive ATP binding site.

(Allada et al., 2003)

Amino acid replacement: M161K. Amino acid replacement: E165D.

(Lin et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:23,102,733..23,102,733 [+]

Nucleotide change:

T23102733A

Amino acid change:

Reported amino acid change:

M161K

Comment:

One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lin et al., 2002)

point_mutation

3L:23,102,746..23,102,746 [+]

Nucleotide change:

A23102746Y

Amino acid change:

Reported amino acid change:

E165D

Comment:

One of two amino acid substitutions observed in mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Lin et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor rhythm

(Akten et al., 2009)

abnormal locomotor rhythm | dominant

(Lin et al., 2002)

lethal | recessive

(Lin et al., 2002)

lethal - all die before end of larval stage | recessive

(Allada et al., 2003)

lethal - all die during pupal stage (with CkIIα^LL07221)

(Bulat et al., 2014)

some die during larval stage | recessive

(Allada et al., 2003)

Phenotype Manifest In

egg chorion | maternal effect

(Wong et al., 2011)

eye, with CkIIα^RNAi.UAS, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

eye (with CkIIα^RNAi.UAS), with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

interommatidial bristle, with CkIIα^RNAi.UAS, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

interommatidial bristle (with CkIIα^RNAi.UAS), with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

macrochaeta, with CkIIα^RNAi.UAS, Scer\GAL4^sca-537.4

(Bose et al., 2006)

macrochaeta (with CkIIα^RNAi.UAS), with Scer\GAL4^sca-537.4

(Bose et al., 2006)

neuromuscular junction (with CkIIα^LL05896)

(Bulat et al., 2014)

ommatidium, with CkIIα^RNAi.UAS, Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

ommatidium (with CkIIα^RNAi.UAS), with Scer\GAL4^ey.3.5.Exel

(Bose et al., 2006)

synapse (with CkIIα^LL05896)

(Bulat et al., 2014)

trichogen cell, with CkIIα^RNAi.UAS, Scer\GAL4^sca-537.4

(Bose et al., 2006)

trichogen cell (with CkIIα^RNAi.UAS), with Scer\GAL4^sca-537.4

(Bose et al., 2006)

Detailed Description

Statement

Reference

CkIIα^P1/CkIIα^Tik transheterozygotes exhibit synaptic retraction in ~40% of neuromuscular junctions in muscles 1/9 and 2/10.

(Bulat et al., 2014)

Approximately 20% of eggs laid by CkIIα^Tik/+ females have ventralised chorions.

(Wong et al., 2011)

The wings of CkIIα^Tik/+ flies appear wild type.

(Jia et al., 2010)

CkIIα^Tik/Y adults have a longer circadian periodicity of locomotor activity than wild-type controls.

(Akten et al., 2009)

Expression of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^ey.3.5.Exel in a CkIIα^Tik/+ background results in a more severe eye phenotype than expression in a wild-type background. Scer\GAL4^ey.3.5.Exel>CkIIα^{dsRNA.Scer\UAS}, CkIIα^Tik/+ eyes are rough in all sections, slightly reduced in size, display fused ommatidia, and have an interommatidial bristle patterning that is more defective.

CkIIα^Tik/+ flies do not display bristle defects.

Expression of one copy of CkIIα^{dsRNA.Scer\UAS} under the control of Scer\GAL4^sca-537.4 in a CkIIα^Tik/+ background results in split bristles and branching of the shaft cell.

(Bose et al., 2006)

Lethality acts in the third larval instar. CkIIα^Tik dominantly lengthens circadian period by nearly three hours. CkIIα^Tik heterozygotes exhibit reduced kinase activity. In CkIIα^Tik there are delays in the disappearance of the products of the per and tim genes, suggesting a role in clock protein turnover.

(Allada et al., 2003)

Heterozygotes have a circadian locomotor activity period of 26.4 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies).

(Lin et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

CkIIα^Tik has abnormal locomotor rhythm phenotype, non-suppressible by S6kII^ign-Δ58-1

(Akten et al., 2009)

Enhancer of

Statement

Reference

CkIIα^Tik/CkIIalpha[+] is an enhancer of visible phenotype of Scer\GAL4^C-765, smo^PKA12.UAS

(Jia et al., 2010)

CkIIα^Tik/CkIIalpha[+] is an enhancer of abnormal locomotor rhythm phenotype of per⁰¹, per^{S149-151-153A.tG}

(Lin et al., 2005)

Suppressor of

Statement

Reference

CkIIα^Tik is a suppressor of abnormal locomotor rhythm phenotype of S6kII^ign-Δ58-1

(Akten et al., 2009)

Phenotype Manifest In

Enhanced by

Statement

Reference

CkIIα^Tik has egg chorion | maternal effect phenotype, enhanceable by orb^F343/orb[+]/Ecol\lacZ^{Hsp83.orb.8E2Hd}

(Wong et al., 2011)

CkIIα^Tik has egg chorion | maternal effect phenotype, enhanceable by CkIIβ^mbuΔA26-2L/CkIIbeta[+]

(Wong et al., 2011)

Enhancer of

Statement

Reference

CkIIα^Tik/CkIIalpha[+] is an enhancer | maternal effect of egg chorion | maternal effect phenotype of Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343

(Wong et al., 2011)

CkIIα^Tik/CkIIalpha[+] is an enhancer of wing vein L3 phenotype of Scer\GAL4^C-765, smo^PKA12.UAS

(Jia et al., 2010)

CkIIα^Tik/CkIIalpha[+] is an enhancer of wing vein L4 phenotype of Scer\GAL4^C-765, smo^PKA12.UAS

(Jia et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Over 60% of eggs laid by CkIIα^Tik/orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343 females have ventralised chorions.

CkIIβ^mbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females to approximately 35%.

(Wong et al., 2011)

CkIIα^Tik/+ enhances the wing phenotype seen when smo^{PKA12.Scer\UAS} is expressed under the control of Scer\GAL4^C-765.

(Jia et al., 2010)

The increased periodicity of circadian locomoter acticvity seen in CkIIα^Tik/Y adults is not suppressed by S6kII^ign-Δ58-1/Y.

(Akten et al., 2009)

CkIIα^Tik, E(spl)¹ double mutant flies do not show ommatidial or interommatidial bristle defects.

(Bose et al., 2006)

The longer periods of circadian locomotor activity seen in per⁰¹; per^{S149-151-153A.tG} flies are lengthened further when these flies are CkIIα^Tik/+.

(Lin et al., 2005)

per^s ; CkIIα^Tik/+ flies have a circadian locomotor activity period of 20.4 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies). per^L1 ; CkIIα^Tik/+ flies have a circadian locomotor activity period of 32.5 +/- 0.1 hours (compared to 23.6 +/- 0.1 hours for wild-type flies).

(Lin et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

80982

w¹¹¹⁸; CkIIα^Tik P{EPgy2}dco^EY02910/TM6C, Sb¹

24512

y¹ w^*; CkIIα^Tik/TM3, P{GAL4-Kr.C}DC2, P{UAS-GFP.S65T}DC10, Sb¹

Notes on Origin

Discoverer

Comments

Based on heterozygous lethality, these alleles of CkIIα can be placed in the following order, from severe to weak: CkIIα^P2 = CkIIα^TikR > CkIIα^P1 > CkIIα^Tik > CkIIα^H3091 > CkIIα^G703.

(Bulat et al., 2014)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CK2α^Tik

(Jia et al., 2010, Lin et al., 2005)

Ck2α^Tik

(Hirschhäuser et al., 2021)

CkIIα^Tik

(Bulat et al., 2014)

Tik

(Akten et al., 2009, Bose et al., 2006)

ck2α^Tik

(Wong et al., 2011)

Name Synonyms

Timekeeper

(Lin et al., 2002)

Secondary FlyBase IDs

References (10)

Report Sections

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