FlyBase Allele Report: Dmel\CkIIβ[mbuΔA26-2L]

General Information

Symbol

Dmel\CkIIβ^mbuΔA26-2L

Species

D. melanogaster

Name

FlyBase ID

FBal0145124

Feature type

allele

Associated gene

Dmel\CkIIβ

Associated Insertion(s)

Carried in Construct

Also Known As

DmCk2β^mbuΔA26-2L

Key Links

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Jauch et al., 2006)

Mutagen

Delta2-3 transposase

(Jauch et al., 2002)

P-element activity

(Akten et al., 2003)

Progenitor genotype

CkIIβ^mbuP1

(Akten et al., 2003, Jauch et al., 2002)

Cytology

Description

Exons I-VI (including most of the coding region), exon VIIb, and most of exon VIIa are deleted in CkIIβ^mbuΔA26-2L.

(Jauch et al., 2006)

Imprecise excision has created a deletion that spans from before exon Ia to the middle of exon VII, removing the entire coding region.

(Akten et al., 2003)

Imprecise excision of the P{lacW} element, resulting in a deletion that removes a large part of CkIIβ.

(Jauch et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal circadian rhythm

(Akten et al., 2003)

abnormal circadian rhythm (with CkIIβ^And)

(Akten et al., 2003)

abnormal neuroanatomy (with CkIIβ^And)

(Jauch et al., 2006)

abnormal neuroanatomy (with CkIIβ^mbuP1)

(Jauch et al., 2006)

lethal

(Jauch et al., 2006)

lethal | recessive

(Jauch et al., 2002)

viable (with CkIIβ^mbuP1)

(Bulat et al., 2014)

Phenotype Manifest In

calyx of adult mushroom body, with CkIIβ^VIIb.UAS, Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

calyx of adult mushroom body (with CkIIβ^And)

(Jauch et al., 2006)

calyx of adult mushroom body (with CkIIβ^mbuP1)

(Jauch et al., 2006)

neuromuscular junction (with CkIIβ^mbuP1)

(Bulat et al., 2014)

neuromuscular junction | somatic clone

(Bulat et al., 2014)

synapse (with CkIIβ^mbuP1)

(Bulat et al., 2014)

synapse | somatic clone

(Bulat et al., 2014)

Detailed Description

Statement

Reference

Transheterozygous CkIIβ^mbuP1/CkIIβ^mbuΔA26-2L mutant larvae show a small but significant increase in synaptic retractions and survive to adulthood.

CkIIβ^mbuΔA26-2L mutant MARCM clones cause synaptic retraction at 33% of the mutant neuromuscular junctions.

(Bulat et al., 2014)

The mushroom body calyx of flies transheterozygous for CkIIβ^mbuP1 and CkIIβ^mbuΔA26-2L is approximately a third of the size of that in wild type flies.

The mushroom body calyx of flies transheterozygous for CkIIβ^And and CkIIβ^mbuΔA26-2L is approximately a third of the size of that in wild type flies.

Expression of CkIIβ^{VIIb.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL in a CkIIβ^mbuΔA26-2L background results in mushroom body calyces that are proportionally larger than those of wild type flies.

Expression of CkIIβ^{VIIa-M166I.Scer\UAS} under the control of Scer\GAL4^αTub84B.PL rescues the mushroom body phenotype of CkIIβ^mbuΔA26-2L flies.

(Jauch et al., 2006)

Heterozygotes have a circadian rhythm of 24.6 hours, CkIIβ^mbuΔA26-2L/CkIIβ^And transheterozygotes 25.8 hours.

(Akten et al., 2003)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

NOT suppressed by

Statement

Reference

CkIIβ^mbuΔA26-2L has phenotype, non-suppressible by Ssl^UAS.cJa/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L has phenotype, non-suppressible by CkIIβ2^UAS.cJa/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

Enhancer of

Statement

Reference

CkIIβ^mbuΔA26-2L/CkIIbeta[+] is an enhancer | maternal effect of egg chorion | maternal effect phenotype of Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343

(Wong et al., 2011)

CkIIβ^mbuΔA26-2L/CkIIbeta[+] is an enhancer of egg chorion | maternal effect phenotype of CkIIα^Tik

(Wong et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

CkIIβ^mbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females to 75%.

CkIIβ^mbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females to approximately 35%.

(Wong et al., 2011)

Expression of Ssl^Scer\UAS.cJa under the control of Scer\GAL4^αTub84B.PL fails to rescue the lethality of CkIIβ^mbuΔA26-2L mutant flies.

Expression of CkIIβ2^Scer\UAS.cJa under the control of Scer\GAL4^αTub84B.PL fails to rescue the lethality of CkIIβ^mbuΔA26-2L mutant flies.

(Jauch et al., 2006)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

CkIIβ^And

(Akten et al., 2003)

Rescued by

CkIIβ^mbuΔA26-2L is rescued by Scer\GAL4^da.G32/CkIIβ^UAS.N.Tag:HA

(Bulat et al., 2014)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^Ie-VIIa.UAS/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^gDNA

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^VIIb.UAS/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is rescued by Scer\GAL4^αTub84B.PL/CkIIβ^VIIc.UAS

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^{VIIa-M166I.UAS}/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^Ie-VIIa.UAS/Scer\GAL4^αTub84B.PL

(Jauch et al., 2002)

CkIIβ^mbuΔA26-2L is rescued by CkIIβ^gDNA

(Jauch et al., 2002)

CkIIβ^mbuΔA26-2L is rescued by Scer\GAL4^αTub84B.PL/CkIIβ^R47C.UAS

(Jauch et al., 2002)

Not rescued by

CkIIβ^mbuΔA26-2L is not rescued by CkIIβ^VIId.UAS/Scer\GAL4^αTub84B.PL

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is not rescued by Scer\GAL4^αTub84B.PL/CkIIβ^VIId-VI.UAS

(Jauch et al., 2006)

CkIIβ^mbuΔA26-2L is not rescued by CkIIβ^{C109.114S.UAS}/Scer\GAL4^αTub84B.PL

(Jauch et al., 2002)

CkIIβ^mbuΔA26-2L is not rescued by CkIIβ^{C137.140S.UAS}/Scer\GAL4^αTub84B.PL

(Jauch et al., 2002)

CkIIβ^mbuΔA26-2L is not rescued by Scer\GAL4^αTub84B.PL/CkIIβ^S2.3.4A.UAS

(Jauch et al., 2002)

Comments

Ubiquitous expression of CkIIβ^{Scer\UAS.N.T:Ivir\HA1} rescues the lethality and synaptic retraction phenotype found in CkIIβ^mbuΔA26-2L mutants.

(Bulat et al., 2014)

Both male and female CkIIβ^mbuΔA26-2L flies rescued by the CkIIβ^gDNA transgene are fertile.

Two copies of the CkIIβ^gDNA transgene resuces the mushroom body phenotype of CkIIβ^mbuΔA26-2L flies.

When expressed under the control of Scer\GAL4^αTub84B.PL, not all of the CkIIβ^{VIIb.Scer\UAS} transgenic lines rescue the lethality of CkIIβ^mbuΔA26-2L flies.

(Jauch et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

35825

w^* CkIIβ^mbuΔA26-2L/FM7a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

CkIIβ^mbu26-2

(Bulat et al., 2014)

CkIIβ^mbuΔA26-2L

DmCK2β^mbuΔA26-2L

(Jauch et al., 2006)

DmCk2β^mbuΔA26-2L

(Jauch et al., 2002)

ck2β^mbuΔA26-2L

(Wong et al., 2011)

mbu26-2

(Akten et al., 2003)

Name Synonyms

Secondary FlyBase IDs

References (5)

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