FlyBase Allele Report: Ecol\lacZ[Hsp83.orb.8E2Hd]

General Information

Symbol

Ecol\lacZ^{Hsp83.orb.8E2Hd}

Species

E. coli

Name

FlyBase ID

FBal0340274

Feature type

allele

Associated gene

Ecol\lacZ

Associated Insertion(s)

Carried in Construct

P{Hsp83-lacZ-orb3'UTR.HD}

Also Known As

HD19G

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Lantz and Schedl, 1994)

Progenitor genotype

Ecol\lacZ^Hsp83.Adh

(Lantz and Schedl, 1994)

Carried in construct

P{Hsp83-lacZ-orb3'UTR.HD}

Cytology

Description

orb 3' UTR and genomic DNA downstream of the poly(A) site are fused downstream of Ecol\lacZ.

(Lantz and Schedl, 1994)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp83

Encoded product / tool

lacZ

reporter enzyme

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

egg chorion

(Tan et al., 2001)

egg chorion, with orb^F343

(Tan et al., 2001)

egg chorion | maternal effect, with orb^F343

(Wong et al., 2011, Costa et al., 2005)

egg chorion | temperature conditional, with orb^F343

(Costa et al., 2013)

Detailed Description

Statement

Reference

When a single copy of orb^{8E2Hd.Hsp83.T:Ecol\lacZ} is introduced into orb^F343/+ females, orb^{8E2Hd.Hsp83.T:Ecol\lacZ} increases the frequency of ventralised eggs. orb^{8E2Hd.Hsp83.T:Ecol\lacZ} rin³/+ females lay between 3-30% ventralised eggs depending on the temperature.

(Costa et al., 2013)

Almost 30% of eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females have ventralised chorions.

(Wong et al., 2011)

4.5% of eggs produced by females carrying one copy of orb^{8E2Hd.Hsp83.T:Ecol\lacZ} have ventralised chorions. 11.6% of eggs produced by females carrying two copies of orb^{8E2Hd.Hsp83.T:Ecol\lacZ} have ventralised chorions. 27.1% of eggs produced by orb^F343/+ females carrying one copy of orb^{8E2Hd.Hsp83.T:Ecol\lacZ} have ventralised chorions. 74.6% of eggs produced by orb^F343/+ females carrying two copies of orb^{8E2Hd.Hsp83.T:Ecol\lacZ} have ventralised chorions.

(Tan et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | temperature conditional phenotype, enhanceable by rin³/rin[+]

(Costa et al., 2013)

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | maternal effect phenotype, enhanceable | maternal effect by CkIIα^Tik/CkIIalpha[+]

(Wong et al., 2011)

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | maternal effect phenotype, enhanceable | maternal effect by CkIIβ^mbuΔA26-2L/CkIIbeta[+]

(Wong et al., 2011)

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | maternal effect phenotype, enhanceable | maternal effect by Fmr1^+t14

(Costa et al., 2005)

NOT Enhanced by

Statement

Reference

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | maternal effect phenotype, non-enhanceable | maternal effect by CkIIα^TikR/CkIIalpha[+]

(Wong et al., 2011)

Suppressed by

Statement

Reference

Ecol\lacZ^{Hsp83.orb.8E2Hd}, orb^F343 has egg chorion | maternal effect phenotype, suppressible | heat sensitive | maternal effect by Fmr1³/Fmr1[+]

(Costa et al., 2005)

Enhancer of

Statement

Reference

orb^F343, orb[+], Ecol\lacZ^{Hsp83.orb.8E2Hd} is an enhancer of egg chorion | maternal effect phenotype of CkIIα^Tik

(Wong et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The frequency of ventralised eggs is increased three-fold or more depending on the temperature when orb^{8E2Hd.Hsp83.T:Ecol\lacZ} rin³ orb^F343/+ mothers are also heterozygous for rin³.

(Costa et al., 2013)

Over 60% of eggs laid by CkIIα^Tik/orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343 females have ventralised chorions.

CkIIα^TikR/+ does not increase the frequency of the ventralised chorion phenotype seen in eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females.

CkIIβ^mbuΔA26-2L/+ increases the frequency of the ventralised chorion phenotype seen in eggs laid by orb^{8E2Hd.Hsp83.T:Ecol\lacZ} orb^F343/+ females to 75%.

(Wong et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58478

w^*; P{Hsp83-lacZ-orb3'UTR.HD}19G, orb^F343 e¹/TM3, Ser¹

Notes on Origin

Discoverer

Comments

orb^{8E2Hd.Hsp83.T:Ecol\lacZ} acts as a dominant negative allele.

(Tan et al., 2001)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Ecol\lacZ^{Hsp83.orb.8E2Hd}

Ecol\lacZ^{orb.8E2Hd.Hsp83}

lacZ^{orb.8E2Hd.Hsp83}

orb^{8E2Hd.Hsp83.T:Ecol\lacZ}

Name Synonyms

Secondary FlyBase IDs

FBal0056623
FBal0340240

References (6)

Report Sections

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