FlyBase Allele Report: Dmel\smo[PKA12.UAS]

General Information

Symbol

Dmel\smo^PKA12.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0264181

Feature type

allele

Associated gene

Dmel\smo

Associated Insertion(s)

Carried in Construct

P{UAS-smo.PKA12}

Also Known As

Smo^-PKA12, UAS-Smo^-PKA12, UAS-Smo^DN

Key Links

Transgenic product class

missense_variant

(Jia et al., 2010)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Jia et al., 2010)

Mutagen

in vitro construct

(Jia et al., 2010)

Progenitor genotype

Carried in construct

P{UAS-smo.PKA12}

Cytology

Description

UAS regulatory sequences drive expression of smo containing mutations at two PKA sites.

(Jia et al., 2010)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

smo

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017, Li et al., 2016)

visible, with Scer\GAL4^C-765

(Fan et al., 2013, Zhang et al., 2013, Jia et al., 2010)

visible | adult stage, with Scer\GAL4^C-765

(Zhang et al., 2017, Chen et al., 2010)

Phenotype Manifest In

wing, with Scer\GAL4^Bx-MS1096

(Wang et al., 2017, Li et al., 2016)

wing, with Scer\GAL4^C-765

(Zhang et al., 2017)

wing blade, with Scer\GAL4^C-765

(Chen et al., 2010)

wing vein, with Scer\GAL4^C-765

(Zhang et al., 2017, Chen et al., 2010)

wing vein L3, with Scer\GAL4^C-765

(Fan et al., 2013, Zhang et al., 2013, Jia et al., 2010)

wing vein L4, with Scer\GAL4^C-765

(Fan et al., 2013, Zhang et al., 2013, Jia et al., 2010)

Detailed Description

Statement

Reference

Expression of smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 causes a fused wing phenotype, with partial fusion of wing veins L3 and L4, as compared to controls.

(Wang et al., 2017)

Expression of smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 results in a partial fusion of vein L3 and L4.

(Zhang et al., 2017)

Expression of smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 results in wing fusion between veins L3 and L4 of the adult wing.

(Li et al., 2016)

Expression of Scer\GAL4^C-765>smo^{PKA12.Scer\UAS} causes a reproducible wing phenotype with partial fusion between wing veins L3 and L4.

(Fan et al., 2013)

Expression of smo^{PKA12.Scer\UAS} in the imaginal wing disc, under the control of Scer\GAL4^C-765 leads to a 'fused wing' phenotype.

(Yang et al., 2013)

The L3 and L4 wing veins are fused in flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765. This phenotype is partially rescued when the flies are fed trichostatin A (TSA) from the fertilized egg stage.

(Zhang et al., 2013)

Expression of smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 results in a 'fused wing' phenotype: partial fusion between vein 3 and vein 4.

(Chen et al., 2010)

Expression of smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 results in a wing phenotype in which there is partial fusion between veins 3 and 4.

(Jia et al., 2010)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by apt^GD1367/Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by hh^ΔaptDB1

(Wang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^C-765/lwr^GD10017

(Zhang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Su(var)2-10^JF01408/Scer\GAL4^C-765

(Zhang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Scer\GAL4^C-765/Sumo^JF02869

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by gish^KK108680/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by gish^JF02903/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by gish^GD10588/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by Df(3R)ED10639/+

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by gish[+]/gish^Δ4

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, enhanceable | somatic clone by gish^KG03891/gish^KG03891

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has visible phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Li et al., 2016)

Scer\GAL4^C-765, smo^PKA12.UAS has visible phenotype, enhanceable by Hrs^RNAi.UAS.cUa, Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has visible phenotype, enhanceable by TSG101^GD14295/Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Gprk2[+]/Gprk2⁰⁶⁹³⁶

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Gprk2^RNAi.UAS.cCa, Scer\GAL4^C-765

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, enhanceable by Df(3R)Exel6219/+

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has visible phenotype, enhanceable by CkIIα^Tik/CkIIalpha[+]

(Jia et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, suppressible | partially by Scer\GAL4^C-765/Ulp1^GD7581

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, suppressible | partially by gish^{UAS.RB.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has visible phenotype, suppressible | partially by gish^{UAS.RB.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^C-765, smo^PKA12.UAS has visible phenotype, suppressible | partially by Hrs^UAS.Tag:HA, Scer\GAL4^C-765

(Fan et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^C-765, smo^PKA12.UAS has visible | adult stage phenotype, non-suppressible by velo^KK112690/Scer\GAL4^C-765

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, non-suppressible by dco^UAS.Tag:FLAG/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, non-suppressible by gish[+]/gish^KG03891

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has visible phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/gish^{UAS.RF.Tag:MYC}

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has visible phenotype, non-suppressible by gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, non-suppressible by gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/gish^{UAS.RF.Tag:MYC}

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has visible phenotype, non-suppressible by CkIα^UAS.Tag:FLAG, Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Other

Statement

Reference

CkIIβ^RNAi.cJa.UAS, Scer\GAL4^C-765, smo^PKA12.UAS has abnormal size phenotype

(Jia et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by apt^GD1367/Scer\GAL4^Bx-MS1096

(Wang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by hh^ΔaptDB1

(Wang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype, enhanceable by Scer\GAL4^C-765/lwr^GD10017

(Zhang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype, enhanceable by Su(var)2-10^JF01408/Scer\GAL4^C-765

(Zhang et al., 2017)

Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype, enhanceable by Scer\GAL4^C-765/Sumo^JF02869

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by gish^KK108680/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by gish^JF02903/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by gish^GD10588/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by Df(3R)ED10639/+

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by gish[+]/gish^Δ4

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, enhanceable | somatic clone by gish^KG03891/gish^KG03891

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has wing phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Li et al., 2016)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L3 phenotype, enhanceable by Hrs^RNAi.UAS.cUa, Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L4 phenotype, enhanceable by Hrs^RNAi.UAS.cUa, Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L3 phenotype, enhanceable by TSG101^GD14295/Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L4 phenotype, enhanceable by TSG101^GD14295/Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has wing blade phenotype, enhanceable by Df(3R)Exel6219/+

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein phenotype, enhanceable by Df(3R)Exel6219/+

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing blade phenotype, enhanceable by Gprk2[+]/Gprk2⁰⁶⁹³⁶

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein phenotype, enhanceable by Gprk2[+]/Gprk2⁰⁶⁹³⁶

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing blade phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein phenotype, enhanceable by Gprk2^Δ15/Gprk2[+]

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing blade phenotype, enhanceable by Gprk2^RNAi.UAS.cCa, Scer\GAL4^C-765

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein phenotype, enhanceable by Gprk2^RNAi.UAS.cCa, Scer\GAL4^C-765

(Chen et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L3 phenotype, enhanceable by CkIIα^Tik/CkIIalpha[+]

(Jia et al., 2010)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L4 phenotype, enhanceable by CkIIα^Tik/CkIIalpha[+]

(Jia et al., 2010)

Suppressed by

Statement

Reference

Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype, suppressible | partially by Scer\GAL4^C-765/Ulp1^GD7581

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, suppressible | partially by gish^{UAS.RB.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has wing phenotype, suppressible | partially by gish^{UAS.RB.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L3 phenotype, suppressible | partially by Hrs^UAS.Tag:HA, Scer\GAL4^C-765

(Fan et al., 2013)

Scer\GAL4^C-765, smo^PKA12.UAS has wing vein L4 phenotype, suppressible | partially by Hrs^UAS.Tag:HA, Scer\GAL4^C-765

(Fan et al., 2013)

NOT suppressed by

Statement

Reference

Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype, non-suppressible by velo^KK112690/Scer\GAL4^C-765

(Zhang et al., 2017)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, non-suppressible by gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/gish^{UAS.RF.Tag:MYC}

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, non-suppressible by CkIα^UAS.Tag:FLAG, Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, non-suppressible by dco^UAS.Tag:FLAG/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, smo^PKA12.UAS has wing phenotype, non-suppressible by gish[+]/gish^KG03891

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has wing phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/gish^{UAS.RF.Tag:MYC}

(Li et al., 2016)

Scer\GAL4^Bx-MS1096, gish[+]/gish^Δ4, smo^PKA12.UAS has wing phenotype, non-suppressible by gish^{D187N.UAS.Tag:MYC}/Scer\GAL4^Bx-MS1096

(Li et al., 2016)

Other

Statement

Reference

CkIIβ^RNAi.cJa.UAS, Scer\GAL4^C-765, smo^PKA12.UAS has wing phenotype

(Jia et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of apt^GD1367 enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

The presence of hh^ΔaptDB1 enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

(Wang et al., 2017)

The wing defects (partial fusion of veins L3 and L4) characteristic for flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 are strongly exacerbated by co-expression of any of the following: lwr^GD10017, Su(var)2-10^JF01408 or smt3^JF02869 (which on their own induce moderately reduced wing size and crinkled appearance) leading to small severely misshapen wings, co-expression of Ulp1^GD7581 partially suppresses the vein fusion, whereas expression of velo^KK112690 has no significant effect on the phenotype.

(Zhang et al., 2017)

Co-expression of gish^KK108680, gish^JF02903 or gish^GD10588 enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

Co-expression of gish^{Scer\UAS.RB.T:Hsap\MYC}, but not gish^{D187N.Scer\UAS.T:Hsap\MYC}, gish^{Scer\UAS.RF.T:Hsap\MYC}, CkIα^{Scer\UAS.T:Zzzz\FLAG} or dco^{Scer\UAS.T:Zzzz\FLAG}, partially suppresses the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096.

gish^KG03891/+ does not modify the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096, but the presence of gish^KG03891/gish^KG03891 clones in the wings of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 leads to a greatly enhanced fused wing phenotype.

Df(3R)ED10639/+, Gprk2^Δ15/+ or gish^Δ4/+ enhances the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096, and the presence of both Gprk2^Δ15/+ and gish^Δ4/+ even further enhances the fused wing phenotype.

The enhancement of the fused wing phenotype of flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^Bx-MS1096 by gish^Δ4/+ is reversed by co-expression of gish^{Scer\UAS.RB.T:Hsap\MYC}, but not gish^{Scer\UAS.RF.T:Hsap\MYC} or gish^{D187N.Scer\UAS.T:Hsap\MYC}.

(Li et al., 2016)

Co-expression of TSG101^GD14295 with smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 enhances the wing vein fusion phenotype resulting from the expression of smo^{PKA12.Scer\UAS}.

Co-expression of Hrs^dsRNA.cUa with smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 results in narrowing and further fusion between wing veins L3 and L4.

(Fan et al., 2013)

The 'fused wing' phenotype (partial fusion between L3 and L4 veins) observed in flies expressing smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 can be enhanced to a varying degree by combination with any of the following: Df(3R)Exel6219, Gprk2⁰⁶⁹³⁶, Gprk2^Δ15 (all in heterozygous state) or by co-expression of Gprk2^{dsRNA.Scer\UAS.cCa} RNAi.

(Chen et al., 2010)

CkIIα^Tik/+ enhances the wing vein phenotype seen when smo^{PKA12.Scer\UAS} is expressed under the control of Scer\GAL4^C-765.

Co-expression of CkIIβ^{dsRNA.cJa.Scer\UAS} and smo^{PKA12.Scer\UAS} under the control of Scer\GAL4^C-765 results in flies that have small wings consisting of the anterior- and posterior-most wing structures.

Co-expression of CkIIβ^{Scer\UAS.T:Zzzz\FLAG} suppresses the small wing phenotype seen when CkIIβ^{dsRNA.cJa.Scer\UAS} and smo^{PKA12.Scer\UAS} are expressed under the control of Scer\GAL4^C-765.

(Jia et al., 2010)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Smo^-PKA12

(Ma et al., 2016, Shi et al., 2014)

Smo^-PKA

(Wang et al., 2017)

Smo^DN

(Li et al., 2016)

smo^{PKA12.Scer\UAS}

smo^PKA12.UAS

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

Open Close