FlyBase Allele Report: Dmel\rux[8]

General Information

Symbol

Dmel\rux⁸

Species

D. melanogaster

Name

FlyBase ID

FBal0035525

Feature type

allele

Associated gene

Dmel\rux

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

amorphic allele - genetic evidence

Mutagen

gamma ray

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Dong et al., 1997, Thomas et al., 1994)

loss of function allele

(Thomas et al., 1997)

Mutagen

gamma ray

(Thomas et al., 1994)

Progenitor genotype

Cytology

Polytene chromosomes normal.

(Thomas et al., 1994)

Description

11bp deletion, predicted to cause a frameshift after amino acid 78 and truncation of the protein after the addition of seven out-of-frame amino acids. Only the N-terminal 25% of the protein is predicted to remain.

(Thomas et al., 1994)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

X:6,038,756..6,038,766 [-]

Comment:

11bp deletion, predicted to cause a frameshift after amino acid 78 and truncation of the protein after the addition of 7 novel amino acids.

(Thomas et al., 1994)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

decreased occurrence of cell division | larval stage, with Scer\GAL4^hs.PB

(Horsfield et al., 1998)

male sterile

(Dong et al., 1997, Thomas et al., 1994)

visible | recessive

(Thomas et al., 1994)

Phenotype Manifest In

eye

(Thomas et al., 1994)

eye disc

(Horsfield et al., 1998)

eye disc morphogenetic furrow

(Thomas et al., 1997)

eye disc morphogenetic furrow | larval stage, with Scer\GAL4^hs.PB

(Horsfield et al., 1998)

photoreceptor cell R8

(Thomas et al., 1994)

S phase | ectopic

(Horsfield et al., 1998)

Detailed Description

Statement

Reference

rux⁸ mutant males show normal spermatocyte fusome morphology.

(Varadarajan et al., 2016)

All cells within the morphogenetic furrow ectopically enter S phase.

(Horsfield et al., 1998)

Entry in the S phase is precocious, all cells reenter the cell cycle. Male germ cells progress through meiosis I and meiosis II normally but then undergo an ectopic round of nuclear division.

(Dong et al., 1997)

Ectopic S phase entry in the morphogenetic furrow. Neither a CycE deficiency or a strong point mutation dominantly suppresses the S phase phenotype.

(Thomas et al., 1997)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Eyes are rough, viability reduced to 10% with respect to wild type. Photoreceptor clusters form in rux⁸ with the same frequency and spacing as wild type. However, the organization of cells within the clusters is usually disrupted. The R8 cell number is often abnormal, and numbers of R1, R3, R4 and R6 are reduced.

(Thomas et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

rux⁸ has male sterile phenotype, suppressible by twe¹/twe[+]

(Thomas et al., 1994)

NOT suppressed by

Statement

Reference

rux⁸ has male sterile phenotype, non-suppressible by stg^AR2/stg[+]

(Thomas et al., 1994)

rux⁸ has male sterile phenotype, non-suppressible by stg^RXT13/stg[+]

(Thomas et al., 1994)

Suppressor of

Statement

Reference

rux⁸ is a suppressor of visible | recessive phenotype of CycE^JP

(Secombe et al., 1998)

rux⁸ is a suppressor of decreased occurrence of cell division | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

Phenotype Manifest In

Suppressed by

Statement

Reference

rux⁸ has phenotype, suppressible by CycA^S(rux)3B

(Dong et al., 1997)

rux⁸ has phenotype, suppressible by Rca1^S(rux)2A

(Dong et al., 1997)

rux⁸ has eye phenotype, suppressible by stg^AR2/stg[+]

(Thomas et al., 1994)

rux⁸ has eye phenotype, suppressible by stg^RXT13/stg[+]

(Thomas et al., 1994)

NOT suppressed by

Statement

Reference

rux⁸ has eye phenotype, non-suppressible by twe¹/twe[+]

(Thomas et al., 1994)

Suppressor of

Statement

Reference

rux⁸ is a suppressor of eye phenotype of CycE^JP

(Secombe et al., 1998)

rux⁸ is a suppressor of eye disc posterior to the morphogenetic furrow | larval stage phenotype of CycE^JP

(Secombe et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

Dominantly suppresses the rough eye phenotype of CycE^JP homozygotes. One copy of rux⁸ has no effect on the reduction in the number of cells entering S phase posterior to the morphogenetic furrow seen in CycE^JP homozygotes.

(Secombe et al., 1998)

Mutations in Rca1 and CycA rescue the sterility.

(Dong et al., 1997)

Flies carrying rux⁸ in a heterozygous stg background show partial rescue of the rough-eye phenotype, but not the male sterility, at 18^oC. Removing one copy of twe rescues the sterility, but not the rough eye phenotype, of rux⁸.

(Thomas et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

rux⁸ is rescued by rux^+t6.0

(Thomas et al., 1994)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

9242

y¹ cv¹ rux⁸/FM7c

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (1)

Reported As

Symbol Synonym

rux⁸

(Varadarajan et al., 2016, Escudero and Freeman, 2007, Tanaka-Matakatsu et al., 2007)

Name Synonyms

Secondary FlyBase IDs

References (10)

Report Sections

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