FlyBase Allele Report: Dmel\Rap1[rvB1]

General Information

Symbol

Dmel\Rap1^rvB1

Species

D. melanogaster

Name

FlyBase ID

FBal0042714

Feature type

allele

Associated gene

Dmel\Rap1

Associated Insertion(s)

Carried in Construct

Also Known As

Rap1^B1, Rap1^rv(R)B1, rv(R)^B1

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Knox and Brown, 2002)

loss of function allele

(Ishimaru et al., 1999)

Mutagen

ethyl methanesulfonate

(Hariharan et al., 1991)

Progenitor genotype

Rap1^R-1

(Hariharan et al., 1991)

Cytology

Description

Nucleotide substitution: C?T.

(Hariharan et al., 1991)

Amino acid replacement: Q43term.

(Hariharan et al., 1991)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:1,861,085..1,861,085 [-]

Nucleotide change:

C1861085T

Amino acid change:

Q43term | Rap1-PA; Q43term | Rap1-PB; Q43term | Rap1-PC

Reported amino acid change:

Q43term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Hariharan et al., 1991)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of larval stage

(Asha et al., 1999)

some die during larval stage

(Asha et al., 1999)

wild-type

(Hariharan et al., 1991)

Phenotype Manifest In

Detailed Description

Statement

Reference

Homozygous clones in the pupal wing intermingle with surrounding wild-type cells (in contrast to wild-type clones which have discrete borders). The cells are often dispersed into wild-type tissue in pairs or groups of 4 cells. The mutant cells still respect the lineage restriction at the anterior-posterior boundary. The mutant cells are not the normal hexagonal shape and the area of the apical, but not the basal, surface of the cells is reduced relative to wild-type wing cells. 82% of mutant cells in clones in the pupal wing have adherens junctions condensed into a contact with just one neighbouring cell, in contrast to wild type where there is an even distribution of adherens junction components around the apical circumference of the cell. Clusters of adherens junctions are seen only at interfaces between mutant cells, and not between a mutant and a wild-type cell. Homozygous clones in late third larval instar wing discs are not dispersed, but adherens junctions components are mislocalised, indicating adherens junction mislocalisation precedes dispersal. Dispersal of the mutant cells is first seen 2 hours after pupariation and is initially more pronounced at the distal end of the evaginating wing.

(Knox and Brown, 2002)

Daily 30 minute heat shocks from day 3 to day 7 of development rescue the larval lethality of R^hs.PA,R^rvB1/Df(3L)R-E. Flies are morphologically wild type - except under minimal heat shock conditions when eyes are roughened. Rescued flies can survive without further heat shock for weeks though show a higher mortality than their wild type siblings. Rescued females stop laying eggs within a day or two of heat shock cessation. The ommatidia of partially rescued flies with roughened eyes do not show additional R7 cells. R^hs.PA,R^rvB1/Df(3L)R-E mutant females, 7 days after cessation of heat shock, show fragmentation of nurse cell and follicular nuclei. Structure of egg chamber is collapsed with cytoskeleton remains in a single spherical mass. Prior to collapse discontinuities of the follicular epithelium are observed, stalk cells are missing between egg chambers, nurse cells can be reduced in number. Clonal analysis indicates a role for R in the follicular epithelium. Comparing clones induced at various stages reveals a role for R in the early stage of egg chamber development, possibly in the germarium.

(Asha et al., 1999)

Wild type eyes. Transheterozygotes with Df(3L)R-E are late larval lethal.

(Hariharan et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Rap1[+]/Rap1^rvB1 is an enhancer of visible phenotype of PDZ-GEF³/PDZ-GEF^k13720

(Singh et al., 2006)

Suppressor of

Statement

Reference

R[+]/Rap1^rvB1 is a suppressor of visible phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

Rap1^rvB1 is a suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

Other

Statement

Reference

PDZ-GEF³/PDZ-GEF^k13720, Rap1[+]/Rap1^rvB1 has decreased cell number | spermatogenesis phenotype

(Wang et al., 2006)

Hsap\RAP1A^hs.PA, Rap1^rvB1 has partially lethal phenotype

(Asha et al., 1999)

Phenotype Manifest In

Enhancer of

Statement

Reference

Rap1[+]/Rap1^rvB1 is an enhancer of eye phenotype of PDZ-GEF³/PDZ-GEF^k13720

(Singh et al., 2006)

Rap1[+]/Rap1^rvB1 is an enhancer of wing phenotype of PDZ-GEF³/PDZ-GEF^k13720

(Singh et al., 2006)

Suppressor of

Statement

Reference

R[+]/Rap1^rvB1 is a suppressor of eye photoreceptor cell | ectopic phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF

(Lee et al., 2002)

Rap1^rvB1 is a suppressor of eye phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

Other

Statement

Reference

PDZ-GEF³/PDZ-GEF^k13720, Rap1[+]/Rap1^rvB1 has spermatheca phenotype

(Singh et al., 2006)

PDZ-GEF³/PDZ-GEF^k13720, R[+]/Rap1^rvB1 has male germline stem cell phenotype

(Wang et al., 2006)

Hsap\RAP1A^hs.PA, Rap1^rvB1 has eye phenotype

(Asha et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

PDZ-GEF^k13720/PDZ-GEF³ females have two spermathecae, but with the introduction of Rap1^rvB1/+ or Rap2l^SH0581/+ extra spermathecal capsule materials are observed in some females; PDZ-GEF^k13720/PDZ-GEF³ wing and eye phenotypes are also enhanced.

(Singh et al., 2006)

Dominantly suppresses the eye phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^GMR.PF.

(Ishimaru et al., 1999)

Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

(Hariharan et al., 1995)

Xenogenetic Interactions

Statement

Reference

Twice daily heat shock of Hsap\RAP1A^hs.PA,R^rvB1/Df(3L)R-E generates adult flies but the eyes are roughened and rescue is not as robust as with R^hs.PA.

(Asha et al., 1999)

Complementation and Rescue Data

Rescued by

Rap1^rvB1 is rescued by Rap1^+t4.5

(Asha et al., 1999)

Partially rescued by

Rap1^rvB1/Df(3L)R-E is partially rescued by Rap1^hs.Tag:MYC

(Asha et al., 1999)

Rap1^rvB1/Df(3L)R-E is partially rescued by Rap1^{S179A.hs.Tag:MYC}

(Asha et al., 1999)

Rap1^rvB1/Df(3L)R-E is partially rescued by Rap1^hs.PA

(Asha et al., 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Revertant.

(Hariharan et al., 1991)

Comments

Fail to produce mitotic clones in the eye, this is consistent with an essential role of R gene product in cell proliferation or cell viability.

(Hariharan et al., 1991)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (8)

Reported As

Symbol Synonym

R^rvB1

Rap1^B1

(Yang et al., 2016, Wang et al., 2006, Knox and Brown, 2002, Ishimaru et al., 1999)

Rap1^BI

(Yang et al., 2016)

Rap1^rv(R)B1

(Singh et al., 2006)

Rap1^rv(R)B1

(Wang et al., 2006, Asha et al., 1999, Li et al., 1997)

Rap1^rvB1

(Wang et al., 2013)

Rap^rv(R)B1

(Lee et al., 2002)

rv(R)^B1

(Hariharan et al., 1995, Hariharan et al., 1991)

Name Synonyms

Secondary FlyBase IDs

References (11)

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