FlyBase Allele Report: Dmel\drl[P3.765]

General Information

Symbol

Dmel\drl^P3.765

Species

D. melanogaster

Name

FlyBase ID

FBal0045519

Feature type

allele

Associated gene

Dmel\drl

Associated Insertion(s)

P{etau-lacZ}drl^P3.765

Carried in Construct

Also Known As

drl^P, lio^drlP

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Callahan et al., 1995)

Progenitor genotype

Associated Insertion(s)

P{etau-lacZ}drl^P3.765

Cytology

Description

P{etau-lacZ} is inserted between nucleotides 449 and 450 (where the drl transcription start site is at nucleotide 487). The insertion sites of P{etau-lacZ}drl^P3.765 and P{lacW}pigeon^P1 are identical.

(Taillebourg and Dura, 1999)

The P{etau-lacZ}drl^P3.765 insertion in drl^P3.765 is inserted at the same nucleotide as the P{lacW}pigeon^P1 insertion.

(Moreau-Fauvarque et al., 1998)

P{lacW}pigeon^P1 is inserted 6bp distal to P{etau-lacZ}drl^P3.765. In both cases the element is transcribed in the opposite direction to the pigeon and drl transcription units.

(Bolwig et al., 1995)

P{etau-lacZ} insertion 47bp upstream from the 5' end of the longest drl cDNA.

(Callahan et al., 1995)

Allele components

Component

Use(s)

Inserted element

P{etau-lacZ}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:MT(btau)

microtubule protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:19,190,341..19,190,341 [+]

(Yoshikawa et al., 2003, Hitier et al., 2000, Bonkowsky et al., 1999, Taillebourg and Dura, 1999, Moreau-Fauvarque et al., 1998, Simon et al., 1998, Speicher et al., 1998, Callahan et al., 1996, Bolwig et al., 1995, Callahan et al., 1995)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

distribution deduced from reporter

Stage

Tissue/Position (including subcellular localization)

Reference

third instar larval stage

embryonic/larval central nervous system | restricted

(Hitier et al., 2001)

third instar larval stage -- pupal stage

glial cell | subset

(Hitier et al., 2001)

Additional Information

Statement

Reference

drl^P3.765 is expressed in a transient glial structure called the 'transient interhemispheric fibrous ring' (TIFR) that persists into early pupal stages but disappear by adulthood.

(Hitier et al., 2001)

Marker for

Reflects expression of

drl

(Hitier et al., 2001)

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal memory

(Moreau-Fauvarque et al., 2002)

female fertile

(Callahan et al., 1995)

male fertile

(Callahan et al., 1995)

uncoordinated (with drl^R343)

(Callahan et al., 1995)

uncoordinated | recessive

(Callahan et al., 1995)

viable

(Callahan et al., 1995)

Phenotype Manifest In

adult central complex

(Moreau-Fauvarque et al., 1998)

adult mushroom body

(Moreau-Fauvarque et al., 1998)

adult mushroom body alpha-lobe

(Simon et al., 1998)

adult mushroom body beta-lobe

(Simon et al., 1998)

axon | embryonic stage

(Speicher et al., 1998)

fan-shaped body

(Simon et al., 1998)

Detailed Description

Statement

Reference

Adult flies show impaired olfactory memory. Retention score is similar to that of pigeon^P1;drl^P1 mutants.

(Moreau-Fauvarque et al., 2002)

Homozygous adults show defects in the mushroom bodies and central complex.

(Moreau-Fauvarque et al., 1998)

drl^P3.765/drl^R343 adults have brain defects. The fan-shaped body is distorted on its dorsal side, where axonal structures replace cell bodies. The α lobes of the mushroom bodies are reduced and the β lobes are fused or juxtaposed. The transient interhemispheric ring (TIFR) - a transient ring of cell projections at the interhemispheric junction in the middle of the brain shows defects in drl^P3.765/drl^R343 old larvae and young pupae.

(Simon et al., 1998)

Homozygous embryos show partial defasciculation of axons in the CNS which normally express drl.

(Speicher et al., 1998)

Homozygous embryos are sluggish and uncoordinated and exhibit neuronal pathfinding defects. The drl neurons cross the midline and reach the contralateral longitudinal connective but they project anteriorly along inappropriate paths and thus fail to form the distinctive DD and DV bundles. In many segments drl neurons can be seen crossing one another and appear less organised within the anterior commissure. drl^P3.765/drl^R343 transheterozygotes display a similar phenotype to drl^P3.765 homozygotes, yet drl neurons of transheterozygotes with drl^R193 display wild type pathfinding.

(Callahan et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

cas³⁹²¹, drl^P3.765 has partially lethal phenotype

(Hitier et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

drl^P3.765 has adult mushroom body beta'-lobe phenotype, enhanceable by cas³⁹²¹

(Hitier et al., 2001)

drl^P3.765 has adult mushroom body beta-lobe phenotype, enhanceable by cas³⁹²¹

(Hitier et al., 2001)

drl^P3.765 has adult brain phenotype, enhanceable by cas³⁹²¹

(Hitier et al., 2001)

drl^P3.765 has ellipsoid body phenotype, enhanceable by cas³⁹²¹

(Hitier et al., 2001)

drl^P3.765 has mushroom body phenotype, enhanceable by cas³⁹²¹

(Hitier et al., 2001)

Other

Statement

Reference

Nrt⁵, drl^P3.765 has axon | embryonic stage phenotype

(Speicher et al., 1998)

Additional Comments

Genetic Interactions

Statement

Reference

In drl^P3.765, cas³⁹²¹ double mutants, fibres abnormally cross the midline above a flattened fan-shaped body in 55% of flies, the mushroom bodies β and β' lobes are fully fused in 33% of flies and the ellipsoid body is ventrally cleaved.

(Hitier et al., 2001)

drl^P3.765/drl^P3.765; Nrt⁵/Nrt⁵ and drl^P3.765/Df(2L)TW130; Nrt⁵/Nrt⁵ embryos show strong misguidance and stalling phenotypes in many hemisegments of axons that normally express drl. In addition, defects are seen in axons that do not normally express drl. Defects in Fas2 expressing axons, associated with the defects in drl expressing axons are also seen. These defects are rescued by Nrt^Scer\UAS.cSa expressed under the control of Scer\GAL4^Mz1277.

(Speicher et al., 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

drl²

(Moreau-Fauvarque et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

The P{etau-lacZ}drl^P3.765 insertion in drl^P3.765 is inserted at the same nucleotide as the P{lacW}pigeon^P1 insertion.

(Moreau-Fauvarque et al., 2002)

Reversion of the P{etau-lacZ} insertion demonstrates this to be the cause of the neuronal phenotype.

(Callahan et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

drl^P3.765

drl^P3765

(Taillebourg and Dura, 1999)

drl^P

(Moreau-Fauvarque et al., 2002, Hitier et al., 2001, Hitier et al., 2000, Taillebourg and Dura, 1999, Simon et al., 1998)

lio^drlP

(Hitier et al., 2001, Hitier et al., 2000)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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