FlyBase Allele Report: Dmel\gcm[UAS.cJa]

General Information

Symbol

Dmel\gcm^UAS.cJa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Jones

FlyBase ID

FBal0045765

Feature type

allele

Associated gene

Dmel\gcm

Associated Insertion(s)

Carried in Construct

P{UAS-gcm.J}

Also Known As

uas-gcm, UASgcm

Key Links

Transgenic product class

cDNA

(Jones et al., 1995)

wild_type

(Jones et al., 1995)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Jones et al., 1995)

wild_type

(Jones et al., 1995)

Mutagen

in vitro construct

(Kim et al., 1998, Jones et al., 1995)

Progenitor genotype

Carried in construct

P{UAS-gcm.J}

Cytology

Description

UASt regulatory sequences drive expression of the full-length 1.9kb gcm-1 cDNA.

(Jones et al., 1995)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

gcm

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^sca-537.4

(Alfonso and Jones, 2002)

abnormal neuroanatomy | third instar larval stage, with Scer\GAL4^repo.PU

(Shklyar et al., 2014)

lethal - all die before end of second instar larval stage, with Scer\GAL4^lama-A8

(Chotard et al., 2005)

some die during second instar larval stage, with Scer\GAL4^lama-A8

(Chotard et al., 2005)

Phenotype Manifest In

adult optic lobe | somatic clone, with Scer\GAL4^Act5C.PI

(Chotard et al., 2005)

bipolar dendrite neuron, with Scer\GAL4^elav-C155

(Jones et al., 1995)

crystal cell primordium, with Scer\GAL4^hs.PB

(Bataille et al., 2005)

embryonic/larval crystal cell, with Scer\GAL4^hs.PB

(Bataille et al., 2005)

embryonic/larval glial cell | ectopic, with Scer\GAL4^sca-537.4

(Alfonso and Jones, 2002)

embryonic brain, with Scer\GAL4^repo.PU

(Shklyar et al., 2014)

larval ventral nerve cord, with Scer\GAL4^repo.PU

(Shklyar et al., 2014)

medulla cortex & glial cell | somatic clone, with Scer\GAL4^Act5C.PI

(Chotard et al., 2005)

outer photoreceptor cell | somatic clone, with Scer\GAL4^Act5C.PI

(Chotard et al., 2005)

Detailed Description

Statement

Reference

The brains of third instar larvae expressing gcm^Scer\UAS.cJa under the control of Scer\GAL4^repo.PU exhibit an abnormal shape with a much longer ventral nerve cord compared to wild type brains.

(Shklyar et al., 2014)

Transient expression of gcm^Scer\UAS.cJa, under the control of Scer\GAL4^hs.PB around stage 9, 10, or 11 represses crystal cell formation.

(Bataille et al., 2005)

Expression of gcm^Scer\UAS.cJa under the control of Scer\GAL4^lama-A8 results in unhealthy larvae. However, these larvae produce no additional glial cells at the expense of neurons within the lamina at the level of the R-cell projection filed or glial precursor cell areas.

Induction of clones in the eye disc which express gcm^Scer\UAS.cJa under the control of Scer\GAL4^Act5C.PI leads to disruption of the formation of R-cells in the eye and, thus, with optic lobe development. Although no excess glial cells are seen in the eye, these clones do induce ectopic glial cells within the medulla cortex.

(Chotard et al., 2005)

Expression of gcm^Scer\UAS.cJa under the control of Scer\GAL4^sca-537.4 in embryos results in an increase in the number of glial cells (as assessed by repo staining) which show glial cell morphologies.

(Alfonso and Jones, 2002)

Transformation of presumptive BD neuron into a glial cell, causing an increase repo expressing cells in the CNS (glial cells).

(Jones et al., 1995)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Expression of gcm^Scer\UAS.cJa under the control of Scer\GAL4^da.G32 partially rescues the hemocyte defects seen in Df(2L)200 embryos.

(Alfonso and Jones, 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^elav-C155, gcm^UAS.cJa, Scer\GAL4^αTub84B.PL rescues Df(2L)200

(Chotard et al., 2005)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5446

y¹ w^67c23; P{UAS-gcm.J}2; P{UAS-gcm.J}3

Kyoto

108239

y¹ w^67c23; P{UAS-gcm.J}2; P{UAS-gcm.J}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

gcm^Scer\UAS.cJa

gcm^UAS.cJa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Jones

(Jones et al., 1995)

Secondary FlyBase IDs

References (16)

Report Sections

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