FlyBase Allele Report: Dmel\Notum[I6]

General Information

Symbol

Dmel\Notum^I6

Species

D. melanogaster

Name

FlyBase ID

FBal0049852

Feature type

allele

Associated gene

Dmel\Notum

Associated Insertion(s)

Carried in Construct

Also Known As

Notum⁵, wf¹⁴¹

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Giraldez, 2002.9.13)

Mutagen

ethyl methanesulfonate

(Gerlitz and Basler, 2002)

Progenitor genotype

Cytology

Description

Amino acid replacement: R141term.

(Gerlitz and Basler, 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:16,029,616..16,029,616 [+]

Nucleotide change:

C16029616T

Amino acid change:

R141term | Notum-PA

Reported amino acid change:

R141term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Gerlitz and Basler, 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal sternite bristle | increased number (with Df(3L)st-f13)

(Gerlitz and Basler, 2002)

denticle row 1

(Desbordes et al., 2005)

dorsocentral bristle | increased number (with Df(3L)st-f13)

(Gerlitz and Basler, 2002)

embryonic/first instar larval cuticle

(Desbordes et al., 2005)

eye

(You et al., 2011)

mechanosensory chaeta | ectopic

(You et al., 2011)

medial row of triple row of wing sensilla | ectopic

(Han et al., 2005)

sensory mother cell | ectopic

(Han et al., 2005)

sensory mother cell | ectopic | somatic clone

(Han et al., 2005)

taste bristle | ectopic

(You et al., 2011)

ventral denticle belt

(Desbordes et al., 2005)

wing disc (with Df(3L)st-f13)

(Gerlitz and Basler, 2002)

wing disc (with Notum³)

(Giraldez et al., 2002)

wing margin

(You et al., 2011)

wing margin bristle | increased number (with Df(3L)st-f13)

(Gerlitz and Basler, 2002)

wing margin bristle | increased number (with Notum^KO)

(Kakugawa et al., 2015)

wing margin taste bristle | increased number

(Magny et al., 2021)

wing sensillum | cell non-autonomous | somatic clone | increased number

(Gerlitz and Basler, 2002)

Detailed Description

Statement

Reference

Notum^I6 mutants exhibit a 20% survival rate.

Notum^I6 heterozygous flies exhibit an increase in chemosensory bristles and ectopic mechanosensory bristles compared to wild-type, although fewer chemosensory bristles and ectopic mechanosensory bristles compared to Notum^I6 homozygotes.

(You et al., 2011)

85% of mutant embryos derived lack denticle row 1 in most denticle belts, and they often have a breach of naked cuticle in segment A1. 10% of mutant embryos have a stronger phenotype, with patches of naked cuticle within denticle belts and sometimes complete replacement of denticle belts by naked cuticle.

(Desbordes et al., 2005)

When Notum^I6 mutant clones are made in the wing disc a ectopic sensory organ precursors are seen. Adult mutant escapers have wings with many ectopic thick mechanosensory bristles and curved chemosensory bristles at the wing margin.

(Han et al., 2005)

Notum^I6/Df(3L)st-f13 larval wing discs are enlarged with an extended wing blade region. Adult escapers show a dramatically increased number of mechanosensory bristles in the wing and have 28 +/- 2 sternite bristles per segment (compared to 19 +/- 2 in wild-type flies). Extra dorsocentral bristles are present on the thorax. Homozygous clones in the wing result in the formation of extra sensory bristles within and adjacent to the clone. Small mutant clones have no phenotype as they are rescued by adjacent wild-type tissue.

(Gerlitz and Basler, 2002)

Notum³/Notum^I6 larval wing discs show defects ranging from duplication of the wing pouch and hinge associated with a reduced thorax region to almost complete loss of the thorax region associated with a severely abnormal wing duplication.

(Giraldez et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Notum^I6, Sulf1^Δ1 has partially lethal - majority die phenotype

(You et al., 2011)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Notum^I6 has wing margin taste bristle | increased number phenotype, non-enhanceable by peg^Del1/peg[+]

(Magny et al., 2021)

Notum^I6 has sensory mother cell | ectopic | somatic clone phenotype, non-enhanceable by dlp^A187

(Han et al., 2005)

NOT suppressed by

Statement

Reference

Notum^I6 has wing margin taste bristle | increased number phenotype, non-suppressible by peg^Del1/peg[+]

(Magny et al., 2021)

Notum^I6 has sensory mother cell | ectopic | somatic clone phenotype, non-suppressible by dlp^A187

(Han et al., 2005)

Enhancer of

Statement

Reference

Notum^I6 is an enhancer of mechanosensory chaeta | increased number phenotype of Sulf1^Δ1

(You et al., 2011)

NOT Suppressor of

Statement

Reference

Notum^I6 is a non-suppressor of sensory mother cell | somatic clone phenotype of dally⁸⁰

(Han et al., 2005)

Other

Statement

Reference

Notum^I6, Scer\GAL4^en-e16E, wg^UAS.cLa has ventral denticle belt phenotype

(Desbordes et al., 2005)

Notum^I6, Scer\GAL4^en-e16E, wg^UAS.cLa has embryonic/first instar larval cuticle phenotype

(Desbordes et al., 2005)

Notum[+]/Notum^I6, Scer\GAL4^en-e16E, wg^UAS.cLa has ventral denticle belt phenotype

(Desbordes et al., 2005)

Notum[+]/Notum^I6, Scer\GAL4^en-e16E, wg^UAS.cLa has embryonic/first instar larval cuticle phenotype

(Desbordes et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Notum^I6 Sulf1^Δ1 double mutants exhibit 90% lethality. The surviving 10% display an increase in chemosensory bristles and ectopic mechanosensory bristles on the wing margin, compared to Notum^I6 single mutants.

(You et al., 2011)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

4117

w¹¹¹⁸; Notum^I6 P{white-un4}71F/TM3, Sb¹

Notes on Origin

Discoverer

Comments

12 alleles isolated in a screen devised to recover lethal mutations uncovered by Df(3L)brm11.

(Melendez et al., 1995)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (11)

Reported As

Symbol Synonym

Not⁵

(Desbordes et al., 2005)

Notum¹⁴¹

Notum⁵

(Silver et al., 2007, Giraldez, 2002.9.13, Giraldez et al., 2002)

Notum^I6

(Magny et al., 2021)

Wingful¹⁴¹

(Silver et al., 2007)

l(3)72CDf

(Giraldez et al., 2002)

l(3)72CDf^I6

(Bloomington Drosophila Stock Center, 1997.12.5)

notum¹⁴¹

(Kakugawa et al., 2015)

unspecified

(Melendez et al., 1995)

wf¹⁴¹

(You et al., 2011, Basler, 2002.7.9, Gerlitz and Basler, 2002)

wif¹⁴¹

(Han et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (12)

Report Sections

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