FlyBase Allele Report: Dmel\HDAC1[326]

General Information

Symbol

Dmel\HDAC1³²⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0051881

Feature type

allele

Associated gene

Dmel\HDAC1

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Mottus et al., 2000)

Mutagen

ethyl methanesulfonate

(Mottus et al., 2000, Sinclair et al., 1983)

Progenitor genotype

Cytology

Description

The mutated residue P204 is perfectly conserved in homologs from S. cerevisiae to H. sapiens, in a region of the protein that is almost perfectly conserved, and that is thought to be required for deacetylase activity.

(Mottus et al., 2000)

Amino acid replacement: P204S.

(Mottus et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:4,628,064..4,628,064 [+]

Nucleotide change:

C4628064T

Amino acid change:

P204S | HDAC1-PA

Reported amino acid change:

P204S

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Mottus et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | larval stage | recessive

(Mottus et al., 2000)

lethal | recessive

(Sinclair et al., 1983)

partially lethal - majority die | dominant | male

(Mottus et al., 2000)

partially lethal - majority die | nutrition conditional (with HDAC1⁰⁴⁵⁵⁶)

(Mottus et al., 2000)

short lived | nutrition conditional (with HDAC1⁰⁴⁵⁵⁶)

(Mottus et al., 2000)

suppressor of variegation

(Czermin et al., 2001)

suppressor of variegation | dominant, with In(1)w^m4

(Doheny et al., 2008, Mottus et al., 2000, Sinclair et al., 1983)

suppressor of variegation | dominant, with In(2R)bw^VDe2

(Sinclair et al., 1983)

suppressor of variegation | dominant, with P{hsp26-pt-T}39C-5

(Doheny et al., 2008)

suppressor of variegation | dominant, with T(2;3)Sb^V

(Mottus et al., 2000, Sinclair et al., 1983)

suppressor of variegation | dominant | female, with In(2R)bw^VDe2

(Mottus et al., 2000)

Phenotype Manifest In

Detailed Description

Statement

Reference

Rpd3³²⁶/+ flies have the normal number of dorsocentral bristles.

(Biryukova and Heitzler, 2008)

One copy of Rpd3³²⁶ strongly suppresses position effect variegation (PEV) at the w locus caused by In(1)w^m4.

One copy of Rpd3³²⁶ strongly suppresses the telomeric position effect (TPE) in stocks carrying a variegating P{hsp26-pt-T}39C-5 insertion at the telomere of the left arm of chromosome two.

(Doheny et al., 2008)

Rpd3³²⁶ suppresses position effect variegation at the w gene caused by In(1)w^m4h.

(Czermin et al., 2001)

Strong suppressor of PEV in In(1)w^m4. Raises the pigment levels of In(1)w^m4 eyes from 5-15% of wild type levels to 60-90% of wild type levels. Significant suppressor of PEV in T(2;3)Sb^V. Significant suppressor of PEV in In(2R)bw^VDe2 in females. In males the effect is slight, being, if anything, an enhancement of the PEV. Lethality principally affects the third larval instar stage. Shows a mild dominant semi-lethal effect on males: viability 88% of expected.

(Mottus et al., 2000)

Dominant suppressor of position effect variegation in eye pigment caused by In(1)w^m4 or In(2R)bw^VDe2. Dominant suppressor of position effect variegation at the Sb locus caused by T(2;3)Sb^V. The suppressor effect on the variegation caused by In(1)w^m4 is substantially reduced or eliminated entirely in male XO flies lacking a Y chromosome. Rpd3³²⁶/Su(var)3-9^unspecified heterozygotes are inviable, suggesting the two mutations are allelic.

(Sinclair et al., 1983)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

HDAC1³²⁶/Rpd3[+], pnr^D1 has dorsocentral bristle | increased number phenotype, enhanceable by CtBP[+]/CtBP^rev19

(Biryukova and Heitzler, 2008)

Enhancer of

Statement

Reference

HDAC1³²⁶/Rpd3[+] is an enhancer of dorsocentral bristle | increased number phenotype of pnr^D1

(Biryukova and Heitzler, 2008)

HDAC1³²⁶/Rpd3[+] is an enhancer of dorsocentral bristle | increased number phenotype of CtBP[+]/CtBP^rev19, pnr^D1

(Biryukova and Heitzler, 2008)

Suppressor of

Statement

Reference

HDAC1³²⁶ is a suppressor of sex comb phenotype of Pc⁴

(Chang et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

Flies heterozygous for Rpd3³²⁶ and pnr^D1 enhance the phenotype of pnr^D1/+, resulting in extra sensory organs.

pnr^D1, CtBP^rev19, Rpd3³²⁶ triple heterozygous mutant flies show enhancement of the dorsocentral bristle phenotype compared with pnr^D1/Rpd3³²⁶.

(Biryukova and Heitzler, 2008)

Rpd3³²⁶ still suppresses position effect variegation at the w gene caused by In(1)w^m4h in a Su(var)3-9^+t11.5 background.

(Czermin et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially complements

HDAC1^15-1

(Mottus et al., 2000)

Fails to complement

HDAC1³⁰³

(Mottus et al., 2000)

HDAC1³¹³

(Mottus et al., 2000)

HDAC1³²⁸

(Mottus et al., 2000)

HDAC1⁰⁴⁵⁵⁶

(Mottus et al., 2000)

Comments

Transheterozygotes of Rpd3^15-1 with Rpd3³⁰³, Rpd3³¹³, Rpd3³²⁶ or Rpd3³²⁸ are viable and fertile, and show weak to moderate suppression of In(1)w^m4 PEV.

(Mottus et al., 2000)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

HDAC1³²⁶

(Biryukova and Heitzler, 2008, Czermin et al., 2001, Mottus et al., 2000)

HDAC³²⁶

(Czermin et al., 2001)

Hdac1³²⁶

(Doheny et al., 2008, Chang et al., 2001)

Rpd3³²⁶

Su(var)326^unspecified

unspecified

(Sinclair et al., 1983)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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