FlyBase Allele Report: Dmel\E(spl)m7-HLH[UAS.cdCa]

General Information

Symbol

Dmel\E(spl)m7-HLH^UAS.cdCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of de Celis

FlyBase ID

FBal0052331

Feature type

allele

Associated gene

Dmel\E(spl)m7-HLH

Associated Insertion(s)

Carried in Construct

P{UAS-E(spl)m7-HLH.C}

Also Known As

UAS-m7, UAS-E(spl)m7

Key Links

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(de Celis et al., 1996)

Mutagen

in vitro construct

(de Celis et al., 1996)

Progenitor genotype

Carried in construct

P{UAS-E(spl)m7-HLH.C}

Cytology

Description

UAS regulatory sequences drive expression of E(spl)m7-HLH.

(de Celis et al., 1996)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

E(spl)m7-HLH

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^455.2

(Apidianakis et al., 1999, Ligoxygakis et al., 1999)

visible, with Scer\GAL4^ap-md544

(Giagtzoglou et al., 2003, Ligoxygakis et al., 1999)

visible, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

visible, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

visible, with Scer\GAL4^pnr-MD237

(Zarifi et al., 2012, Ahmed et al., 2003, Giagtzoglou et al., 2003)

visible, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

visible, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

Phenotype Manifest In

adult mesothoracic segment, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

adult thorax, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

anterior wing margin, with Scer\GAL4^bi-md653

(Kux et al., 2013)

chaeta, with Scer\GAL4^ap-md544

(Giagtzoglou et al., 2003)

chaeta, with Scer\GAL4^pnr-MD237

(Zarifi et al., 2012)

eye, with Scer\GAL4^GMR.PF

(Chandra et al., 2003)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Chandra et al., 2003)

leg & macrochaeta, with Scer\GAL4^30A

(Jimenez and Ish-Horowicz, 1997)

macrochaeta, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

macrochaeta, with Scer\GAL4^sca-537.4

(Nagel et al., 2000)

mesothoracic tergum, with Scer\GAL4^ap-md544

(Giagtzoglou et al., 2003)

mesothoracic tergum, with Scer\GAL4^pnr-MD237

(Zarifi et al., 2012)

mesothoracic tergum & macrochaeta, with Scer\GAL4^sca-C253

(Hasson et al., 2005)

microchaeta, with Scer\GAL4^pnr-MD237

(Giagtzoglou et al., 2003)

scutellar bristle, with Scer\GAL4^455.2

(Apidianakis et al., 1999, Ligoxygakis et al., 1999)

scutellar bristle, with Scer\GAL4^dpp.blk1

(Li et al., 2009)

scutellar bristle, with Scer\GAL4^pnr-MD237

(Ahmed et al., 2003)

scutellar bristle, with Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

scutellum & macrochaeta, with Scer\GAL4^h-1J3

(Jimenez and Ish-Horowicz, 1997)

scutum & macrochaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

scutum & macrochaeta, with Scer\GAL4^h-1J3

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

scutum & microchaeta, with Scer\GAL4^ap-md544

(Ligoxygakis et al., 1999)

sensory mother cell, with Scer\GAL4^sca-C253

(Hasson et al., 2005)

wing margin bristle, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing margin bristle, with Scer\GAL4^bi-md653

(Kux et al., 2013)

wing vein, with Scer\GAL4^32B

(Ligoxygakis et al., 1999)

wing vein, with Scer\GAL4^en.PU

(Kux et al., 2013)

wing vein L2, with Scer\GAL4^69B

(Jimenez and Ish-Horowicz, 1997)

wing vein L4, with Scer\GAL4^69B

(Jimenez and Ish-Horowicz, 1997)

Detailed Description

Statement

Reference

Scer\GAL4^en.PU-mediated expression of E(spl)m7-HLH^{Scer\UAS.cdCa} deletes wing veins.

Scer\GAL4^bi-md653-mediated expression of E(spl)m7-HLH^{Scer\UAS.cdCa} deletes anterior wing margin bristles.

(Kux et al., 2013)

The eyes of flies overexpressing E(spl)m7-HLH^{Scer\UAS.cdCa} under the control of Scer\GAL4^GMR.PU do not show any detectable abnormalities.

(Xie et al., 2012)

Expression of E(spl)m7-HLH^{Scer\UAS.cdCa} under the control of Scer\GAL4^pnr-MD237 eliminates bristle formation in the Scer\GAL4^pnr-MD237-expression domain of the adult thorax.

(Zarifi et al., 2012)

Flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^dpp.blk1 have missing scutellar bristles.

(Li et al., 2009)

Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^sca-C253 leads to bristle loss on the notum, following suppression of sensory organ precursor cells.

(Hasson et al., 2005)

Adults expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^pnr-MD237 results in a bald mesothorax region.

(Ahmed et al., 2003)

When HLHm7^{Scer\UAS.cdCa} is driven by Scer\GAL4^GMR.PF, most bristles in the eye are lost. While the ommatidia remain highly organised, bristle sockets are present only frequently or are entirely missing. If present, sockets are mispositioned and sometimes duplicated.

(Chandra et al., 2003)

Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^pnr-MD237 inhibits the formation of both macro- and micro-chaetae, resulting in a bald stripe in the centre of the adult thorax. Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^ap-md544 abolishes bristles throughout the notum.

(Giagtzoglou et al., 2003)

Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^ey.PB has no effect on either eye or antenna disc development.

(Kumar and Moses, 2001)

When HLHmδ^{Scer\UAS.cdCa} is driven by Scer\GAL4^sca-537.4 bristle los is observed on the notum.

(Nagel et al., 2000)

Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^ptc-559.1 causes no scutellar bristles to be formed. Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^455.2 causes no scutellar bristles to be formed.

(Apidianakis et al., 1999)

Flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B show loss of wing veins. Loss of notum microchaetae and severe loss of wing margin bristles is seen. Flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^ap-md544 show complete loss of notum microchaetae and macrochaetae, except at the very anterior edge of the notum. Flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^455.2 show severe loss of scutellar bristles. Flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^h-1J3 show loss of notum macrochaetae.

(Ligoxygakis et al., 1999)

Scer\GAL4^h-1J3-mediated expression causes loss of bristles on the scutellum. Scer\GAL4^30A-mediated expression causes loss of bristles on the leg. Scer\GAL4^69B-mediated expression suppresses wing veins L2 and L4.

(Jimenez and Ish-Horowicz, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237 has visible phenotype, non-enhanceable by fred^RNAi.UAS, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

NOT suppressed by

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237 has visible phenotype, non-suppressible by fred^RNAi.UAS, Scer\GAL4^pnr-MD237

(Chandra et al., 2003)

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has visible phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^455.2 has visible phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)m7-HLH^UAS.cdCa is an enhancer of visible | recessive | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^GMR.PU is a suppressor | partially of visible phenotype of Scer\GAL4^GMR.PU, uif^asterisk.UAS

(Xie et al., 2012)

Phenotype Manifest In

Suppressed by

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^sca-C253 has mesothoracic tergum & macrochaeta phenotype, suppressible by btl::Egfr^λ.UAS, Scer\GAL4^sca-C253

(Hasson et al., 2005)

NOT suppressed by

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^ptc-559.1 has scutellar bristle phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^ptc-559.1

(Apidianakis et al., 1999)

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^455.2 has scutellar bristle phenotype, non-suppressible by E(spl)m4-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

Enhancer of

Statement

Reference

Scer\GAL4^32B/E(spl)m7-HLH^UAS.cdCa is an enhancer of wing | heat sensitive phenotype of N^l1N-ts1

(Ligoxygakis et al., 1999)

Suppressor of

Statement

Reference

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^GMR.PU is a suppressor | partially of eye phenotype of Scer\GAL4^GMR.PU, uif^asterisk.UAS

(Xie et al., 2012)

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^pnr-MD237 is a suppressor of chaeta | ectopic phenotype of Scer\GAL4^pnr-MD237, sc^UAS.cPa

(Giagtzoglou et al., 2003)

E(spl)m7-HLH^UAS.cdCa/Scer\GAL4^klu-G410 is a suppressor of proneural cluster phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

NOT Suppressor of

Statement

Reference

E(spl)m7-HLH^UAS.cdCa/Scer\GAL4^klu-G410 is a non-suppressor of wing disc phenotype of Su(H)⁸/Su(H)²

(Klein et al., 2000)

E(spl)m7-HLH^UAS.cdCa, Scer\GAL4^455.2 is a non-suppressor of scutellar bristle | ectopic phenotype of E(spl)m4-BFM^UAS.cAa, Scer\GAL4^455.2

(Apidianakis et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The rough and small eye phenotype resulting from the expression of uif^{asterisk.Scer\UAS} is significantly alleviated by co-expression of E(spl)m7-HLH^{Scer\UAS.cdCa}.

(Xie et al., 2012)

Coexpression of btl::Egfr^{Scer\UAS.T:λ\cI-DD} with HLHm7^{Scer\UAS.cdCa}, under the control of Scer\GAL4^sca-C253, suppresses the bristle-loss phenotype on the notum seen when HLHm7^{Scer\UAS.cdCa} alone is expressed.

(Hasson et al., 2005)

Adults expressing both ed^Ext.Scer\UAS and HLHm7^{Scer\UAS.cdCa} simultaneously under the control of Scer\GAL4^pnr-MD237 show the same loss of bristles phenotype as flies expressing HLHm7^{Scer\UAS.cdCa} alone under the control of Scer\GAL4^pnr-MD237.

(Ahmed et al., 2003)

When HLHm7^{Scer\UAS.cdCa} is added fred^{dsRNA.Scer\UAS}, Scer\GAL4^pnr-MD237 animals, no effect is seen on the HLHm7^{Scer\UAS.cdCa} phenotype.

(Chandra et al., 2003)

Co-expression of HLHm7^{Scer\UAS.cdCa} and sc^Scer\UAS.cPa under the control of Scer\GAL4^pnr-MD237 results in adult flies with a bald stripe in the centre of the thorax (the formation of ectopic chaetae caused by expression of sc^Scer\UAS.cPa under the control of Scer\GAL4^pnr-MD237 is suppressed in these flies). gro^E48 clones induced in flies expressing HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^ap-md544 result in patches of high bristle density in a bald notum.

(Giagtzoglou et al., 2003)

HLHm7^{Scer\UAS.cdCa} expressed under the control of Scer\GAL4^32B enhances the wing notching seen in Df(1)N-54l9 heterozygotes. Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B enhances the scalloped wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the early third larval instar stage (72-84 hours after egg laying). Expression of HLHm7^{Scer\UAS.cdCa} under the control of Scer\GAL4^32B has no effect on the nicked wing phenotype seen in hemizygous N^l1N-ts1 flies shifted to the non-permissive temperature at the mid third larval instar stage (96-108 hours after egg laying).

(Ligoxygakis et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

26681

y¹ w^*; P{UAS-E(spl)m7-HLH.C}2

Notes on Origin

Discoverer

Comments

The relative ability of UAS constructs of the E(spl) complex to induce wing vein deletion when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHmβ^{Scer\UAS.cdCa} = HLHmγ^Scer\UAS.cLa > E(spl)^Scer\UAS.cNa = HLHm7^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum microchaetae when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} >> HLHmγ^Scer\UAS.cLa = HLHmβ^{Scer\UAS.cdCa} = HLHm3^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of wing margin bristles when expressed under the control of Scer\GAL4^32B is (ranked according to severity of phenotype): HLHm7^{Scer\UAS.cdCa} > E(spl)^Scer\UAS.cNa > HLHmγ^Scer\UAS.cLa > HLHmδ^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa}. The relative ability of UAS constructs of the E(spl) complex to induce loss of notum macrochaetae when expressed under the control of Scer\GAL4^h-1J3 is (ranked according to severity of phenotype): E(spl)^Scer\UAS.cNa > HLHm7^{Scer\UAS.cdCa} > HLHmβ^{Scer\UAS.cdCa} > HLHmδ^{Scer\UAS.cdCa} > HLHmγ^Scer\UAS.cLa = HLHm3^Scer\UAS.cLa.

(Ligoxygakis et al., 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

E(spl)m7-HLH^{Scer\UAS.cdCa}

E(spl)m7-HLH^UAS.cdCa

HLHm7^{Scer\UAS.cdCa}

HLHm7^UAS.cdCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Celis

(de Celis et al., 1996)

Secondary FlyBase IDs

References (26)

Report Sections

Open Close