FB2024_03 , released June 25, 2024
Allele: Dmel\B52UAS.cKa
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General Information
Symbol
Dmel\B52UAS.cKa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Kraus
FlyBase ID
FBal0059376
Feature type
allele
Associated gene
Dmel\B52
Associated Insertion(s)
Carried in Construct
P{UAS-B52.K}
Also Known As
UAS-B52, UASB52
Key Links
Transgenic product class
cDNA
(Kraus and Lis, 1994)
wild_type
(Kraus and Lis, 1994)
Mutagen
Nature of the Allele
Transgenic product class
cDNA
(Kraus and Lis, 1994)
wild_type
(Kraus and Lis, 1994)
Mutagen
in vitro construct
(Kraus and Lis, 1994)
Progenitor genotype
Carried in construct
P{UAS-B52.K}
Cytology
Description

UASt regulatory sequences drive expression of a B52 cDNA.

(Kraus and Lis, 1994)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
B52
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Flies expressing B52Scer\UAS.cKa under the control of Scer\GAL4dsx.PU do not show impaired development of sex-specific features.

      (Zaharieva et al., 2015)

      Expression of B52Scer\UAS.cKa under the control of Scer\GAL4ey.PU results in eye defects; 33% of eyes show slight disorganisation, 19% have are smaller than normal and 2% are completely missing.

      Expression of B52Scer\UAS.cKa under the control of Scer\GAL4GMR.PF results in strongly reduced eyes.

      (Juge et al., 2010)

      All females expressing B52Scer\UAS.cKa under the control of Scer\GAL4GMR.PF die as late pupae and less than 1% of males expressing B52Scer\UAS.cKa under the control of Scer\GAL4GMR.PF eclose. Male survivors have weak retina defects (this phenotype is fully penetrant). Flies expressing B52Scer\UAS.cKa under the control of Scer\GAL4sca-537.4 are viable but fail to develop bristles.

      (Labourier et al., 1999)

      When expression is governed by Scer\GAL4hs.PB sternopleural bristle number is reduced. When expression is governed by Scer\GAL4dpp.blk1 salivary glands are 100% undeveloped and wings 0% are unfolded (100% remain folded). When expression is governed by Scer\GAL4G-17 40% of abdominal sternites show a defect. When expression is governed by Scer\GAL4I-65 there is no survival beyond the 2nd larval instar.

      (Shi et al., 1999)

      Scer\GAL4A-25-mediated expression causes lethality during larval and pupal stages allowing a few adults to survive. Scer\GAL4A-27- and Scer\GAL4C-70-mediated expression causes lethality during larval and pupal stages. Adult survivors from Scer\GAL4G-17 expression have abdominal sternite defects. Scer\GAL4I-86-mediated expression causes abdominal sternite defects in adults. Scer\GAL4hs.PB-mediated expression causes bristle defects, wing defects and slower egg production in adults.

      (Kraus and Lis, 1994)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressed by
      Suppressor of
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of Top1Scer\UAS.cJa strongly suppresses the eye defects caused by expression of B52Scer\UAS.cKa under the control of Scer\GAL4ey.PU; 96% of eyes are wild type, and 4% show slight disorganisation in the double mutants.

      Co-expression of Top1Scer\UAS.cJa partly suppresses the mutant eye phenotype caused by expression of B52Scer\UAS.cKa under the control of Scer\GAL4GMR.PF.

      (Juge et al., 2010)

      Flies expressing both B52Scer\UAS.cKa and Rsf1Scer\UAS.cLa under the control of Scer\GAL4GMR.PF survive to adulthood and have retina defects. Flies expressing both B52Scer\UAS.cKa and Rsf1Scer\UAS.cLa under the control of Scer\GAL4sca-537.4 are viable and show complete (10% of flies) or partial (90% of flies) rescue of the sensory bristle pattern.

      (Labourier et al., 1999)
      Xenogenetic Interactions
      Statement
      Reference

      When expression is governed by Scer\GAL4hs.PB in combination with Zzzz\aptamerB52.BBS.Scer\UAS the bristle number is restored to near normal morphology. When expression is governed by Scer\GAL4dpp.blk1 in combination with Zzzz\aptamerB52.BBS.Scer\UAS the salivary glands are restored to near normal morphology and the wings are restored such that 20% are unfolded. When expression is governed by Scer\GAL4G-17 in combination with Zzzz\aptamerB52.BBS.Scer\UAS less than 5% of abdominal sternites are defective. When expression is governed by Scer\GAL4I-65 in combination with Zzzz\aptamerB52.BBS.Scer\UAS 60% of the flies survive to adulthood.

      (Shi et al., 1999)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      B52Scer\UAS.cKa
      B52UAS.cKa
      Name Synonyms
      Saccharomyces cerevisiae UAS construct a of Kraus
      (Kraus and Lis, 1994)
      Secondary FlyBase IDs
        References (7)