FlyBase Allele Report: Dmel\Hsc70-3[K97S.UAS]

General Information

Symbol

Dmel\Hsc70-3^K97S.UAS

Species

D. melanogaster

Name

FlyBase ID

FBal0060647

Feature type

allele

Associated gene

Dmel\Hsc70-3

Associated Insertion(s)

Carried in Construct

P{UAS-Hsc70-3.K97S}

Also Known As

UAS-Hsc70-3^K97S

Key Links

Transgenic product class

missense_variant

(Elefant and Palter, 1999)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

missense_variant

(Elefant and Palter, 1999)

Mutagen

in vitro construct

(Elefant and Palter, 1999, Elefant and Palter, 1997)

Progenitor genotype

Hsc70-3^UAS.cEa

(Elefant and Palter, 1999)

Carried in construct

P{UAS-Hsc70-3.K97S}

Cytology

Description

Amino acid replacement: K97S.

(Elefant and Palter, 1999)

Encodes a Hsc70-3 protein with a single point mutation in the ATP binding site.

(Elefant and Palter, 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Hsc70-3

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Huntington's disease

modeled by Hsap\HTT^{128Q.1-336.UAS}

(Branco et al., 2008)

exacerbates olivopontocerebellar atrophy

modeled by Hsap\ATXN1^82Q.UAS

(Branco et al., 2008)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | adult stage, with Scer\GAL4^GH146

(Sekine et al., 2013)

lethal, with Scer\GAL4^how-24B

(Elefant and Palter, 1999)

lethal | dominant, with Scer\GAL4^unspecified

(Elefant and Palter, 1997)

lethal - all die before end of larval stage, with Scer\GAL4^337Y

(Elefant and Palter, 1999)

some die during larval stage, with Scer\GAL4^337Y

(Elefant and Palter, 1999)

Phenotype Manifest In

adult antennal lobe projection neuron, with Scer\GAL4^GH146

(Sekine et al., 2013)

eye, with Scer\GAL4^GMR.PF

(Chang et al., 2004)

ommatidium, with Scer\GAL4^GMR.PF

(Chang et al., 2004)

Detailed Description

Statement

Reference

Expressing Hsc70-3^K97S.UAS under the control of Scer\GAL4^elav-C155 does not induce bang sensitivity.

(Mori et al., 2019)

Projection neurons expressing Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^GH146 show mild defects in targeting.

(Sekine et al., 2013)

Expression of Hsc70-3^{K97S.Scer\UAS} in the eye discs under the regulation of Scer\GAL4^GMR.PF causes a slight deformation and roughening of the eye.

(Chang et al., 2004)

Animals expressing Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^337Y die as first or second instar larvae. Animals expressing Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^how-24B generally die as pupae or larvae (depending on the P{UAS-Hsc70-3.K97S} line used).

(Elefant and Palter, 1999)

Dominant lethal allele.

(Elefant and Palter, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Hsc70-3^K97S.UAS/Scer\GAL4^GH146 is an enhancer of abnormal neuroanatomy | adult stage | somatic clone phenotype of meigo¹

(Sekine et al., 2013)

Suppressor of

Statement

Reference

Hsc70-3^K97S.UAS/Scer\GAL4^arm.PS is a suppressor | partially of neurogenic phenotype | maternal effect | embryonic stage phenotype of pcx³

(Yamakawa et al., 2012)

NOT Suppressor of

Statement

Reference

Scer\GAL4^elav-C155/Hsc70-3^K97S.UAS is a non-suppressor of bang sensitive phenotype of iPLA2-VIA^null

(Mori et al., 2019)

Phenotype Manifest In

Enhancer of

Statement

Reference

Hsc70-3^K97S.UAS/Scer\GAL4^GH146 is an enhancer of adult antennal lobe projection neuron | somatic clone phenotype of meigo¹

(Sekine et al., 2013)

Hsc70-3^K97S.UAS, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\HTT^{128Q.1-336.UAS}, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Hsc70-3^K97S.UAS, Scer\GAL4^GMR.PU is an enhancer of retina phenotype of Hsap\ATXN1^82Q.UAS, Scer\GAL4^GMR.PU

(Branco et al., 2008)

Suppressor of

Statement

Reference

Hsc70-3^K97S.UAS/Scer\GAL4^arm.PS is a suppressor of endoplasmic reticulum | maternal effect phenotype of pcx³

(Yamakawa et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Expression of Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^GH146 enhances the targeting defects seen in homozygous meigo¹ projection neuron clones.

(Sekine et al., 2013)

Expression of Hsc70-3^{K97S.Scer\UAS} under the control of Scer\GAL4^arm.PS in pcx³ embryos which lack maternal and zygotic pcx function partially rescues the neurogenic phenotype: 29% of embryos are fully rescued, 38% are partially rescued and 33% are not rescued. The endoplasmic reticulum defects are also rescued in 67% of cases.

(Yamakawa et al., 2012)

Coexpression of Hsc70-3^{K97S.Scer\UAS} and Rme-8^{fl.Scer\UAS.T:Disc\RFP-mRFP}, both under the regulation of Scer\GAL4^GMR.PF has little or no effect on the Hsc70-3^{K97S.Scer\UAS} phenotype.

(Chang et al., 2004)

Xenogenetic Interactions

Statement

Reference

Expression of Hsc70-3^{K97S.Scer\UAS} enhances the retinal phenotype seen when Hsap\HTT^{128Q.1-336.Scer\UAS} is expressed under the control of Scer\GAL4^GMR.PU.

Expression of Hsc70-3^{K97S.Scer\UAS} enhances the retinal phenotype seen when Hsap\ATXN1^82Q.Scer\UAS is expressed under the control of Scer\GAL4^GMR.PU.

(Branco et al., 2008)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5842

w¹²⁶; P{UAS-Hsc70-3.K97S}D

Kyoto

108460

w¹²⁶; P{UAS-Hsc70-3.K97S}D

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

HSC3^K97S

(Elefant and Palter, 1997)

Hsc3^K97S

(Sekine et al., 2013)

Hsc70-3^{K97S.Scer\UAS}

(Branco et al., 2008)

Hsc70-3^K97S.UAS

K97S

(Elefant and Palter, 1995)

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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