FlyBase Allele Report: Dmel\pb[Rev3.HSPB]

General Information

Symbol

Dmel\pb^Rev3.HSPB

Species

D. melanogaster

Name

FlyBase ID

FBal0060955

Feature type

allele

Associated gene

Dmel\pb

Associated Insertion(s)

Carried in Construct

P{HSPB^sans.yeux}

Also Known As

HSPB^Rev3

Key Links

Genomic Maps

JBrowse

Transgenic product class

missense_variant

(Benassayag et al., 1997)

Mutagen

ethyl methanesulfonate

Nature of the Allele

Transgenic product class

missense_variant

(Benassayag et al., 1997)

Mutagen

ethyl methanesulfonate

(Benassayag et al., 1997, Benassayag et al., 1997)

Progenitor genotype

pb^hs.PB

(Benassayag et al., 1997, Benassayag et al., 1997)

Carried in construct

P{HSPB^sans.yeux}

Cytology

Description

Amino acid replacement: R?H. The amino acid replacement is at position 5 with respect to the beginning of the homeobox.

(Benassayag et al., 2003)

Nucleotide substitution: G118A. Amino acid replacement: R202H. In exon 4.

(Benassayag et al., 1997)

Point mutation (CGC to CAC), changes the Arg at the N terminus (5th residue) of the homeodomain to His.

(Benassayag et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:6,711,911..6,711,911 [-]

Nucleotide change:

G6711911A

Reported nucleotide change:

G118A

Amino acid change:

R202H | pb-PA; R197H | pb-PB; R197H | pb-PC; R192H | pb-PD

Reported amino acid change:

R202H

(Benassayag et al., 1997)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | female

(Benassayag et al., 1997)

visible

(Callaerts et al., 2001)

visible | male

(Benassayag et al., 1997)

visible | recessive

(Benassayag et al., 1997)

visible | recessive | heat sensitive

(Benassayag et al., 1997)

Phenotype Manifest In

antenna | heat sensitive

(Benassayag et al., 1997)

antennal sense organ | ectopic

(Benassayag et al., 1997)

eye

(Benassayag et al., 2003, Callaerts et al., 2001)

eye | heat sensitive

(Benassayag et al., 1997, Benassayag et al., 1997)

third segment of antenna

(Benassayag et al., 1997)

Detailed Description

Statement

Reference

2 copies of pb^Rev3.HSPB leads to flies with a slightly reduced eye. Four copies leads to a complete deletion of the eye.

(Benassayag et al., 2003)

Flies carrying pb^Rev3.HSPB show eye loss.

(Callaerts et al., 2001)

Flies carrying one copy of pb^Rev3.HSPB are wild type. Flies carrying two copies of pb^Rev3.HSPB have a single ectopic fine bristle on antennal segment 3. Heat shock causes marked reductions in the eye in flies carrying two copies of pb^Rev3.HSPB.

(Benassayag et al., 1997)

Flies appear wild type in the absence of heat shock. One copy of the transgene causes no visible phenotype. Increasing the copies to four causes reduction in eye tissue until flies have no eyes and antenna take on a more rectangular form carrying a single fine bristle.

(Benassayag et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

pb^Rev3.HSPB has visible phenotype, enhanceable by ey[+]/ey^D1Da

(Callaerts et al., 2001)

pb^Rev3.HSPB has visible phenotype, enhanceable by ey[+]/ey^JD

(Callaerts et al., 2001)

Phenotype Manifest In

Enhanced by

Statement

Reference

pb^Rev3.HSPB has eye phenotype, enhanceable by ey^JD

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by eyg^M3-12

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by Df(4)BA

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey²

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey^D1Da

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey^EH

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey^l1

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey[+]/ey^D1Da

(Callaerts et al., 2001)

pb^Rev3.HSPB has eye phenotype, enhanceable by ey[+]/ey^JD

(Callaerts et al., 2001)

NOT Enhanced by

Statement

Reference

pb^Rev3.HSPB has eye phenotype, non-enhanceable by eya²

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, non-enhanceable by eya^cli-IID

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, non-enhanceable by eyg^c1

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, non-enhanceable by so¹

(Benassayag et al., 2003)

pb^Rev3.HSPB has eye phenotype, non-enhanceable by so^L31

(Benassayag et al., 2003)

Other

Statement

Reference

ey^JD, pb^Rev3.HSPB has antenna phenotype

(Benassayag et al., 2003)

ey^JD, pb^Rev3.HSPB has maxillary palp phenotype

(Benassayag et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The combination of 2 copies of ey^JD with pb^Rev3.HSPB often leads to flies with complete eye loss and the fusion of the antenna and maxillary palp. In some cases, the maxillary palp, remains adjoined to the antennal appendage. However differentiation od the proboscis is not affected. The addition of ey^EH, or ey^D1Da to flies with 2 copies of pb^Rev3.HSPB, leads to a strong enhancement of the eye loss phenotype. The addition of ey², or Df(4)BA to flies with 2 copies of pb^Rev3.HSPB, leads to an enhancement of the eye loss phenotype. The addition of ey^l1, or eyg^M3-12 to flies with 2 copies of pb^Rev3.HSPB, leads to a weak enhancement of the eye loss phenotype. The addition of so¹, so^L31, eya², eya^cli-IID or eyg^c1 to flies with 2 copies of pb^Rev3.HSPB, does not enhance the eye loss phenotype.

(Benassayag et al., 2003)

ey^JD and ey^D1Da dominantly enhance the eye loss phenotype of pb^Rev3.HSPB flies.

(Callaerts et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to rescue

pb^Rev3.HSPB fails to rescue pb⁴

(Benassayag et al., 1997)

pb^Rev3.HSPB fails to rescue pb⁴

(Benassayag et al., 1997)

pb^Rev3.HSPB fails to rescue pb⁵

(Benassayag et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Loss of function allele in the mouthparts and aristae and gain of function in the proximal antenna and eyes.

(Benassayag et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

HPSB^Rev3

(Callaerts et al., 2001)

HSPB^Rev3

(Benassayag et al., 1997, Benassayag et al., 1997)

HSPB<up>sans yeux</up>

(Benassayag et al., 1997)

HSPB^sy

(Benassayag et al., 1997)

pb^Rev3.HSPB

Name Synonyms

Secondary FlyBase IDs

References (5)

Report Sections

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