FlyBase Allele Report: Dmel\Tl[Mhc.PR]

General Information

Symbol

Dmel\Tl^Mhc.PR

Species

D. melanogaster

Name

Myosin heavy chain promoter construct of Rose

FlyBase ID

FBal0061574

Feature type

allele

Associated gene

Dmel\Tl

Associated Insertion(s)

Carried in Construct

P{Mhc'-Tl}

Key Links

Transgenic product class

cDNA

(Rose et al., 1997)

wild_type

(Rose et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Rose et al., 1997)

wild_type

(Rose et al., 1997)

Mutagen

in vitro construct

(Rose et al., 1997)

Progenitor genotype

Carried in construct

P{Mhc'-Tl}

Cytology

Description

Muscle-specific promoter from Mhc drives expression of Tl cDNA.

(Rose et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Mhc

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy

(Ritzenthaler and Chiba, 2003, Suzuki et al., 2000, Rose and Chiba, 1999, Rose et al., 1997)

abnormal neuroanatomy | embryonic stage

(Inaki et al., 2010)

viable

(Rose and Chiba, 1999)

Phenotype Manifest In

abdominal posterior fascicle & growth cone

(Rose et al., 1997)

abdominal segment motor neuron

(Inaki et al., 2010)

filopodium & abdominal ventral longitudinal muscle 3

(Ritzenthaler and Chiba, 2003)

larval VL3/4 motor neuron

(Ritzenthaler and Chiba, 2003, Rose and Chiba, 1999)

RP3 neuron & growth cone

(Suzuki et al., 2000)

Detailed Description

Statement

Reference

The nerve terminals synapsed to muscle M12 are greatly reduced in size compared to controls in embryos expressing Tl^Mhc.PR. The projection of other motor nerves (such as the ISN and SNa) appears normal.

(Inaki et al., 2010)

The growth cone of the RP3 neuron often stalls near its target muscles in embryos expressing Tl^Mhc.PR, failing to retract its filopodia and initiate synaptogenesis. The muscle 6/7 cleft is innervated in only 53% of hemisegments by the end of embryogenesis. Myopodia extending from muscle 6 are initially present at numbers similar to wild type (at 12 hours), but myopodia clustering can only be detected in 26% of hemisegments at 13 hours (compared to 46% of hemisegments in wild type).

(Ritzenthaler and Chiba, 2003)

The RP3 growth cone stalls underneath the muscle 6/7 cleft in 16 hour Tl^Mhc.PR embryos (which ectopically express Tl in muscles 6 and 7) and synaptogenesis does not occur. In 87% of cases the RP3 growth cone remains stalled underneath the 6/7 cleft during hours 16-18. The cleft itself is wider than in wild type and is crowded with unlabelled axonal growth cones from the SNb/d motor pathway at 16 hours (in contrast to this stage in the wild type, where the 6/7 cleft is mainly occupied by the RP3 growth cone). Most neuromuscular innervation outside of the 6/7 cleft appears normal.

(Suzuki et al., 2000)

Embryos expressing Tl^Mhc.PR in the muscles show no difference in the number or overall morphology of muscles. Embryos carrying Tl^Mhc.PR show virtually wild-type-like overall development of the nervous system. However, the RP3 neuron often fails to reach the muscle 6/7 cleft. In most cases, the growth cone stalls just beneath and external to the cleft and fails to initiate synaptogenesis.

(Rose and Chiba, 1999)

SNa growth cones develop normally, SNb growth cones look immature on muscle 6, 7 and 12. 6/7 cleft is often missing. RP3 growth cones reaches just below the correct target, cleft between muscle 6 and 7, but fails to innervate normally due to inhibition of synaptogenesis initiation.

(Rose et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Fas3^Mhc.PC, Tl^Mhc.PR has viable phenotype

(Rose and Chiba, 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Tl^Mhc.PR has larval VL3/4 motor neuron phenotype, suppressible by Fas3^Mhc.PC

(Rose and Chiba, 1999)

Suppressor of

Statement

Reference

Tl^Mhc.PR is a suppressor of larval VL3/4 motor neuron phenotype of Fas3^Mhc.PC

(Rose and Chiba, 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos expressing both Fas3^Mhc.PC and Tl^Mhc.PR in the muscles show no difference in the number or overall morphology of muscles. In contrast to embryos expressing either Fas3^Mhc.PC or Tl^Mhc.PR alone, the muscle 6/7 cleft remains innervated at a high frequency in embryos expressing both Fas3^Mhc.PC and Tl^Mhc.PR and the morphology of the innervation is very similar to wild type. The few RP3 growth cones that do not innervate the 6/7 cleft either stall beneath the 6/7 cleft (the most common phenotype in embryos expressing Tl^Mhc.PR alone) or mistarget to other muscles (a common phenotype in embryos expressing Fas3^Mhc.PC alone). Varying the dosage of Fas3^Mhc.PC and Tl^Mhc.PR indicates that there is an inverse relationship between the level of Tl/Fas3 dosage disparity and the frequency of muscle 6/7 innervation.

(Rose and Chiba, 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Tl^Mhc.PR

Name Synonyms

Myosin heavy chain promoter construct of Rose

(Rose et al., 1997)

Secondary FlyBase IDs

References (5)

Report Sections

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