FB2024_03 , released June 25, 2024
Allele: Dmel\RhoLN25.UAS
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General Information
Symbol
Dmel\RhoLN25.UAS
Species
D. melanogaster
Name
FlyBase ID
FBal0061655
Feature type
allele
Associated gene
Dmel\RhoL
Associated Insertion(s)
Carried in Construct
P{UAS-RhoL.N25}
Also Known As
UAS-RhoLN25
Key Links
Transgenic product class
dominant_negative_variant
(Murphy and Montell, 1996)
missense_variant
(Murphy and Montell, 1996)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(Murphy and Montell, 1996)
missense_variant
(Murphy and Montell, 1996)
Mutagen
in vitro construct
(Murphy and Montell, 1996)
Progenitor genotype
Carried in construct
P{UAS-RhoL.N25}
Cytology
Description

UASt regulatory sequences drive expression of RhoL that carries the amino acid replacement T25N. This is expected to result in a dominant-negative protein (the analagous change in Ras causes preferential binding of GDP compared to GTP).

(Murphy and Montell, 1996)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
RhoL
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of RhoLN25.Scer\UAS under the control of Scer\GAL4elav.PU (using tub-Gal80[ts] to limit the expression to the adult stage) does not significantly alter 3 hr memory of adult flies in an aversive olfactory assay compared to controls.

      (Shuai et al., 2010)

      Expression of RhoLN25.Scer\UAS in border cells, driven by Scer\GAL4slbo.2.6, causes a border cell cluster migration defect in 30% of egg chambers.

      (Medioni and Noselli, 2005)

      Expression of RhoLN25.Scer\UAS under the control of Scer\GAL4gcm-rA87.P or Scer\GAL4da.G32 does not interfere with macrophage migration in the embryo.

      (Paladi and Tepass, 2004)

      RhoLN25.Scer\UAS when driven by Scer\GAL4nos.UTR.T:Hsim\VP16 has no effect on transepithelial migration of germ cells in the embryo.

      (Kunwar et al., 2003)

      Scer\GAL4-mediated expression fails to cause any border cell migration defects.

      (Murphy and Montell, 1996)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      NOT Suppressor of
      Statement
      Reference

      RhoLN25.UAS is a non-suppressor of visible | adult stage phenotype of Scer\GAL4GMR.PU, pblDH-PH.UAS.Tag:HA

      (van Impel et al., 2009)
      Phenotype Manifest In
      NOT Suppressor of
      Statement
      Reference

      RhoLN25.UAS is a non-suppressor of eye | adult stage phenotype of Scer\GAL4GMR.PU, pblDH-PH.UAS.Tag:HA

      (van Impel et al., 2009)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Co-expression of RhoLN25.Scer\UAS does not suppress the rough eye phenotype seen when pblDH-PH.Scer\UAS.T:Ivir\HA1 is expressed under the control of Scer\GAL4GMR.PU.

      (van Impel et al., 2009)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      Comments
      Comments

      Generates a dominant negative protein.

      (Murphy and Montell, 1996)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      DrhoLN25
      (Medioni and Noselli, 2005)
      RhoLN25.Scer\UAS
      RhoLN25.UAS
      Name Synonyms
      Secondary FlyBase IDs
        References (7)