Flies expressing perBG.T:Ppyr\LUC are able to entrain to a 12 hour:12 hour LD cycle at 25oC. These flies entrain to a 12 hour:12 hour 25oC:18oC temperature cycle in LL but exhibit maximum activity during the middle of the warm phase, while the wild-type activity peak is at the end of the warm phase and extends into the phase. perBG.T:Ppyr\LUC flies can also entrain to two consecutive warm:cold cycles in LL where the second cycle is phase advanced by 6 hours; they reach a new stable phase relationship after 3-4 days by steadily advancing their average activity peak during the warm phase.
nocte1, perBG.pLUC has abnormal locomotor behavior phenotype
Flies expressing perBG.T:Ppyr\LUC in a nocte1 background are able to entrain to a 12 hour:12 hour LD cycle at 25oC. In contrast to wild-type flies, perBG.T:Ppyr\LUC nocte1 flies fail to entrain to a 12 hour:12 hour 25oC:18oC temperature cycle in LL; these flies are constantly active throughout both temperatures, except for a drop in activity during a ~1 hour window shortly after transition to the cold phase. Activity levels are higher in the warm phase. Further, the flies fail to entrain to two consecutive warm:cold cycles in LL where the second cycle is phase advanced by 6 hours, instead they abruptly change their activity level at the time of temperature transition. The flies behave similarly to control flies when kept in constant darkness at a variety of constant temperatures.
