FlyBase Allele Report: Dmel\AGO1[k00208]

General Information

Symbol

Dmel\AGO1^k00208

Species

D. melanogaster

Name

FlyBase ID

FBal0064637

Feature type

allele

Associated gene

Dmel\AGO1

Associated Insertion(s)

P{lacW}AGO1^k00208

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Roch et al., 1998, BDGP Project Members, 1994-1999)

Progenitor genotype

Associated Insertion(s)

P{lacW}AGO1^k00208

Cytology

Description

P{lacW} insertion in the first intron of the CT20708 transcript of AGO1, 560bp downstream of the 5' end of the transcript. The insertion maps 1849bp upstream of the 5' end of the CT42234 transcript of AGO1.

(Misra, 2000.8.25)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:13,957,531..13,957,531 [+]

(Lee et al., 2015, Misra, 2000.8.25, Spradling et al., 1999, Roch et al., 1998, BDGP Project Members, 1994-1999)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal | recessive

(Spradling et al., 1999)

lethal - all die before end of larval stage | recessive

(Roch et al., 1998)

some die during larval stage | recessive

(Roch et al., 1998)

Phenotype Manifest In

imaginal disc

(Roch et al., 1998)

Detailed Description

Statement

Reference

Mutants exhibit visible disc abnormalities: very small or no discs. Clones induced in wild type discs are abnormal: clones are small and generated at a lower than expected frequency.

(Roch et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

AGO1[+]/AGO1^k00208 is an enhancer of visible phenotype of sti^Z3-5829

(Sweeney et al., 2008)

Other

Statement

Reference

AGO1[+]/AGO1^k00208, Patr-1^d3 has abnormal neuroanatomy | third instar larval stage phenotype

(Pradhan et al., 2012)

Phenotype Manifest In

Enhancer of

Statement

Reference

AGO1[+]/AGO1^k00208 is an enhancer of eye phenotype of sti^Z3-5829

(Sweeney et al., 2008)

Other

Statement

Reference

AGO1[+]/AGO1^k00208, Patr-1^d3 has embryonic/larval neuromuscular junction | third instar larval stage phenotype

(Pradhan et al., 2012)

AGO1[+]/AGO1^k00208, Patr-1^d3 has type Ib terminal bouton | increased number | third instar larval stage phenotype

(Pradhan et al., 2012)

Additional Comments

Genetic Interactions

Statement

Reference

Patr-1^d3/AGO1^k00208 double heterozygous larvae show a significant increase in the number of type 1b boutons at the muscle 6/7 neuromuscular junction compared to controls.

(Pradhan et al., 2012)

The sti^Z3-5829 rough eye phenotype is dominantly enhanced by AGO1^k00208.

(Sweeney et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

10470

y¹ w^67c23; P{lacW}AGO1^k00208/CyO, P{Car20y}EW1

Kyoto

111119

y^d2 w¹¹¹⁸ P{ey-FLP.N}2 P{5xglBS-lacZ.38-1}TPN1; P{neoFRT}42D P{lacW}AGO1^k00208 /CyO y⁺

Notes on Origin

Discoverer

I. Kiss.

(BDGP Project Members, 1994-1999)

Comments

Complements: mam⁰⁴⁶¹⁵.

(BDGP Project Members, 1994-1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

2/8

(Roch et al., 1998)

AGO1^k00208

Ago1^k00208

(Sweeney et al., 2008)

ago1^l(2)k00208

(Pradhan et al., 2012)

l(2)k00208

(Misra, 2000.8.25, Spradling et al., 1999)

l(2)k00208^k00208

(BDGP Project Members, 1994-1999)

Name Synonyms

Secondary FlyBase IDs

References (8)

Report Sections

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