FlyBase Allele Report: Dmel\wg[UAS.cHa]

General Information

Symbol

Dmel\wg^UAS.cHa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Hays

FlyBase ID

FBal0065587

Feature type

allele

Associated gene

Dmel\wg

Associated Insertion(s)

Carried in Construct

P{UAS-wg.H}

Also Known As

UAS-wg, UAS-wg⁺, UASWg

Key Links

Transgenic product class

wild_type

(Hays et al., 1997)

Mutagen

Nature of the Allele

Transgenic product class

wild_type

(Hays et al., 1997)

Mutagen

FLPase

(Steitz et al., 1998, Hays et al., 1997)

in vitro construct

(Dierick and Bejsovec, 1998)

Progenitor genotype

wg^FRT.UAS.cHa

(Steitz et al., 1998, Hays et al., 1997)

Carried in construct

P{UAS-wg.H}

Cytology

Description

The FRT cassette has been excised, juxtaposing the UASt regulatory sequences and wg coding sequences.

(Hays et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

Also carries

FRT

recombinase target site

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neurophysiology | larval stage

(Ataman et al., 2008)

Phenotype Manifest In

denticle, with Scer\GAL4^e22c

(McCartney et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^e22c

(McCartney et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^en-e16E

(Moline et al., 1999)

embryonic/first instar larval cuticle, with Scer\GAL4^hs.PB

(Gallet et al., 1998)

embryonic/first instar larval cuticle, with Scer\GAL4^prd.RG1

(Moline et al., 1999)

embryonic/first instar larval cuticle | dorsal, with Scer\GAL4^VP16.arm

(McEwen et al., 2000)

embryonic epidermis | dorsal, with Scer\GAL4^VP16.arm

(McEwen et al., 2000)

embryonic midgut constriction 2, with Scer\GAL4^how-24B

(Steitz et al., 1998)

larval dorsal hair, with Scer\GAL4^VP16.arm

(McEwen et al., 2000)

motor neuron | larval stage

(Ataman et al., 2008)

pseudotrachea | ectopic, with Scer\GAL4^dpp.blk1

(Yasunaga et al., 2006)

ventral denticle belt, with Scer\GAL4^en-e16E

(Moline et al., 1999)

ventral denticle belt, with Scer\GAL4^prd.RG1

(Moline et al., 1999)

Detailed Description

Statement

Reference

Overexpression of wg^Scer\UAS.cHa by Scer\GAL4^C380 results in an increase of ghost boutons upon 5X K[+] spaced depolarisation, similar to controls, but 3X K[+] spaced depolarisation also results in an increase in ghost boutons unlike with controls.

Neuromuscular junctions in which wg^Scer\UAS.cHa is overexpressed by Scer\GAL4^C380 show on average no difference in the mEJP (spontaneous excitatory potentials) frequency when stimulated with 5X K[+] spaced depolarisation, but show a shift toward higher mEJP frequencies when compared in a probability plot.

(Ataman et al., 2008)

Expression of wg^Scer\UAS.cHa under the control of Scer\GAL4^dpp.blk1 results in the formation of ectopic pseudotrachea.

(Yasunaga et al., 2006)

When expression is driven by Scer\GAL4^{arm.T:Hsim\VP16}, embryos show puckering of dorsal epidermis with loss of dorsal hair identity and a naked scar at the dorsal midline.

(McEwen et al., 2000)

The denticle belts are replaced with an almost uniform expanse of naked cuticle when wg^Scer\UAS.cHa is expressed under the control of Scer\GAL4^e22c.

(McCartney et al., 1999)

Expression of wg^Scer\UAS.cHa under the control of Scer\GAL4^prd.RG1 results in the ablation of a portion of the even-numbered denticle belts by excess naked cuticle. Expression of wg^Scer\UAS.cHa under the control of Scer\GAL4^en-e16E results in only subtle changes to the normal denticle pattern and some excess naked cuticle.

(Moline et al., 1999)

Overexpression of wg^Scer\UAS.cHa in embryos using Scer\GAL4^hs.PB between stages 7 and 10 results in a naked cuticle phenotype.

(Gallet et al., 1998)

Embryos expressing wg^Scer\UAS.cHa under the control of Scer\GAL4^how-24B have an abnormal second midgut constriction.

(Steitz et al., 1998)

Expression of wg^Scer\UAS.cHa using Scer\GAL4^e22c in wg^l-17 embryos causes all ventral epidermal cells to secrete naked cuticle. wg^l-17 embryos expressing wg^Scer\UAS.cHa under the control of Scer\GAL4^wg.PM show full rescue of the ventral cuticle pattern, although the embryos are slightly distorted as the dorsal pattern elements are not rescued.

(Hays et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressor of

Statement

Reference

Scer\GAL4^prd.RG1/wg^UAS.cHa is a suppressor of ventral denticle belt phenotype of Scer\GAL4^prd.RG1, shi^K44A.UAS

(Moline et al., 1999)

Scer\GAL4^prd.RG1/wg^UAS.cHa is a suppressor of embryonic/first instar larval cuticle phenotype of Scer\GAL4^prd.RG1, shi^K44A.UAS

(Moline et al., 1999)

Scer\GAL4^en-e16E/wg^UAS.cHa is a suppressor of denticle row 1 phenotype of Scer\GAL4^en-e16E, shi^K44A.UAS

(Moline et al., 1999)

Scer\GAL4^en-e16E/wg^UAS.cHa is a suppressor of denticle row 2 phenotype of Scer\GAL4^en-e16E, shi^K44A.UAS

(Moline et al., 1999)

Scer\GAL4^en-e16E/wg^UAS.cHa is a suppressor of denticle row 3 phenotype of Scer\GAL4^en-e16E, shi^K44A.UAS

(Moline et al., 1999)

Scer\GAL4^en-e16E/wg^UAS.cHa is a suppressor of ventral denticle belt phenotype of Scer\GAL4^en-e16E, shi^K44A.UAS

(Moline et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Embryos expressing wg^Scer\UAS.cHa under the control of Scer\GAL4^da.G32 in a otk³ background have no denticles.

(Peradziryi et al., 2011)

The phenotype caused when shi^{K44A.Scer\UAS} is expressed at moderate levels under the control of Scer\GAL4^prd.RG1 can be rescued by co-expression of wg^Scer\UAS.cHa; the naked cuticle region in odd-numbered segments is restored to its normal expanse and the even-numbered denticle belts show a more normal degree of diversity. Expression of shi^{K44A.Scer\UAS} under the control of Scer\GAL4^en-e16E perturbs denticle type specification in the first three rows of denticles in each segment. The phenotype can be rescued by co-expression of wg^Scer\UAS.cHa.

(Moline et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

wg^UAS.cHa is rescued by wg^l-12/Scer\GAL4^futsch-C380

(Ataman et al., 2008)

Rescues

wg^UAS.cHa/Scer\GAL4^futsch-C380 rescues wg^l-12

(Ataman et al., 2008)

wg^UAS.cHa/Scer\GAL4^wg.PM rescues wg^l-17

(Hays et al., 1997)

Partially rescues

Scer\GAL4^prd.RG1/wg^UAS.cHa partially rescues wg^l-17

(Bejsovec and Chao, 2012)

Comments

Expression of wg^Scer\UAS.cHa under the control of Scer\GAL4^prd.RG1 in wg^l-17 embryos fully rescues naked cuticle in odd-numbered segments and substantially rescues denticle diversity in the epidermal cells adjacent to the Scer\GAL4^prd.RG1 expression domain.

(Bejsovec and Chao, 2012)

wg^l-12/+ raised at the restrictive temperature fail to show the increase in ghost boutons seen in controls upon spaced 5X K[+] depolarisation. Expression of wg^Scer\UAS.cHa using Scer\GAL4^C380 in the wg^l-12/+ background rescues this phenotype.

The enhancement in numbers of ghost boutons in Scer\GAL4^C380/+;;wg^Scer\UAS.cHa/+ mutants upon 3X K[+] spaced depolarisation is suppressed with wg^l-12.

(Ataman et al., 2008)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Exists in clones, not reported as being germ-line transmitted.

(Hays et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

wg^Scer\UAS.cHa

wg^UAS.cHa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Hays

(Hays et al., 1997)

Secondary FlyBase IDs

References (21)

Report Sections

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