FlyBase Allele Report: Dmel\H[hs.PM]

General Information

Symbol

Dmel\H^hs.PM

Species

D. melanogaster

Name

heat shock construct of Maier

FlyBase ID

FBal0066044

Feature type

allele

Associated gene

Dmel\H

Associated Insertion(s)

Carried in Construct

P{hs-H.FL}

Also Known As

hs-H

Key Links

Transgenic product class

cDNA

(Maier et al., 1997)

wild_type

(Maier et al., 1997)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Maier et al., 1997)

wild_type

(Maier et al., 1997)

Mutagen

in vitro construct

(Maier et al., 1997)

Progenitor genotype

Carried in construct

P{hs-H.FL}

Cytology

Description

Full length H cDNA construct (including the first intron in the untranslated leader) is expressed from an Hsp70 promoter.

(Maier et al., 1997)

Allele components

Component

Use(s)

Regulatory region(s)

Hsp70

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible | heat sensitive

(Johannes and Preiss, 2002)

visible | heat sensitive, with H^C2.hs

(Johannes and Preiss, 2002)

wild-type

(Maier et al., 1997)

Phenotype Manifest In

macrochaeta

(Maier et al., 1997)

tormogen cell

(Maier et al., 1997)

wing & macrochaeta

(Maier et al., 1997)

wing | heat sensitive

(Johannes and Preiss, 2002)

wing | heat sensitive, with H^C2.hs

(Johannes and Preiss, 2002)

wing vein

(Maier et al., 1997)

wing vein | ectopic | heat sensitive

(Johannes and Preiss, 2002)

wing vein | ectopic | heat sensitive, with H^C2.hs

(Johannes and Preiss, 2002)

Detailed Description

Statement

Reference

Overexpression of H^hs.PM can produce a small amount of ectopic vein material in the wing. Co-expression of H^hs.PM and H^C2.hs results in a synergistic phenotype with strong induction of ectopic wing veins.

(Johannes and Preiss, 2002)

Heat induced expression causes bristle duplications and socket to shaft transformations. Wing veins are thicker, deltas appear at the wing margin and bristles are twinned along the wing margin.

(Maier et al., 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT Enhanced by

Statement

Reference

H^hs.PM has visible phenotype, non-enhanceable by dx^hs.PB

(Nagel et al., 2000)

NOT suppressed by

Statement

Reference

H^hs.PM has visible phenotype, non-suppressible by dx^hs.PB

(Nagel et al., 2000)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

H^hs.PM has macrochaeta phenotype, non-enhanceable by dx^hs.PB

(Nagel et al., 2000)

NOT suppressed by

Statement

Reference

H^hs.PM has macrochaeta phenotype, non-suppressible by dx^hs.PB

(Nagel et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

the addition of dx^hs.PB has no effect on the bristle phenotype seen in H^hs.PM flies.

(Nagel et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

H^hs.PM rescues H^P8

(Maier et al., 2002)

H^hs.PM rescues H^P41

(Maier et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Carried in plasmid "FL" and expressed in S2 cell culture to test for proper protein expression.

(Maier et al., 1997)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

H^hs.PM

Name Synonyms

heat shock construct of Maier

(Maier et al., 1997)

Secondary FlyBase IDs

References (7)

Report Sections

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