FlyBase Allele Report: Dmel\emc[UAS.cdCa]

General Information

Symbol

Dmel\emc^UAS.cdCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of de Celis

FlyBase ID

FBal0082960

Feature type

allele

Associated gene

Dmel\emc

Associated Insertion(s)

Carried in Construct

P{UAS-emc}

Also Known As

UAS-emc, UAS-emc5.3

Key Links

Transgenic product class

cDNA

(Baonza et al., 2000)

wild_type

(Baonza et al., 2000)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Baonza et al., 2000)

wild_type

(Baonza et al., 2000)

Mutagen

in vitro construct

(Baonza et al., 2000, Gupta and Rodrigues, 1997)

Progenitor genotype

Carried in construct

P{UAS-emc}

Cytology

Description

UASt regulatory sequences drive expression of a full length emc cDNA.

(Baonza et al., 2000)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

emc

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

partially lethal - majority die, with Scer\GAL4^Act.PU, emc^AP6/emc^Δ1

(Li and Baker, 2018)

visible, with Scer\GAL4¹⁷⁹

(Gupta and Rodrigues, 1997)

visible, with Scer\GAL4^unspecified

(Baonza et al., 2000)

Phenotype Manifest In

antennal segment 3 sense organ, with Scer\GAL4¹⁷⁹

(Gupta and Rodrigues, 1997)

egg chamber, with Scer\GAL4^c306

(Adam and Montell, 2004)

interommatidial bristle | somatic clone, with Scer\GAL4^Act5C.PU

(Popova et al., 2011)

microchaeta, with Scer\GAL4^Act.PU, emc^AP6/emc^Δ1

(Li and Baker, 2018)

wing margin bristle, with Scer\GAL4^Act.PU, emc^AP6/emc^Δ1

(Li and Baker, 2018)

wing vein | ectopic, with Scer\GAL4^unspecified

(Baonza et al., 2000)

Detailed Description

Statement

Reference

emc^Δ1/emc^AP6 mutants rescued by the expression of emc^UAS.cdCa under the control of Scer\GAL4^Act.PU survive to pharate adults, and a small proportion emerge as adults; both adults and pharate adults exhibit almost normal eye development, including the interommatidial bristles, an essentially normal pattern of thoracic macrochaetae, despite the lack of most microchaetae, a spaced pattern of some microchaetae, and a reduced by essentially normal pattern of wing margin bristles, as compared to controls.

(Li and Baker, 2018)

Flies expressing emc^{Scer\UAS.cdCa} in clones in the eye under the control of Scer\GAL4^Act5C.PU have eyes which lack bristles.

(Popova et al., 2011)

Expression of emc^{Scer\UAS.cdCa} under the control of Scer\GAL4^c306 results in egg chambers containing multiple germline cysts with high penetrance.

(Adam and Montell, 2004)

Expression of emc^{Scer\UAS.cdCa} under the control of Scer\GAL4^unspecified occasionally results in ectopic wing veins.

(Baonza et al., 2000)

Scer\GAL4¹⁷⁹-mediated expression reduced the number of all olfactory sensilla on the antenna.

(Gupta and Rodrigues, 1997)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

emc^UAS.cdCa, Scer\GAL4^Bx-MS1096 is an enhancer of visible phenotype of E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096

(Baonza et al., 2000)

Other

Statement

Reference

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has visible | somatic clone phenotype

(Popova et al., 2011)

Phenotype Manifest In

Enhancer of

Statement

Reference

emc^UAS.cdCa, Scer\GAL4^Bx-MS1096 is an enhancer of wing vein phenotype of E(spl)mβ-HLH^UAS.cdCa, Scer\GAL4^Bx-MS1096

(Baonza et al., 2000)

Other

Statement

Reference

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has eye | somatic clone phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has cone cell | somatic clone | pupal stage phenotype

(Popova et al., 2011)

Rbf^120a, Scer\GAL4^Act5C.PU, emc^UAS.cdCa has eye photoreceptor cell | somatic clone | pupal stage phenotype

(Popova et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

Rbf^120a flies expressing emc^{Scer\UAS.cdCa} in clones in the eye under the control of Scer\GAL4^Act5C.PU have rough eyes which are reduced in size. Pupal eye discs of these animals analysed at 42 hours after puparium formation often contain ommatidia with less than four cone cells per cluster and fewer than normal photoreceptor cells.

(Popova et al., 2011)

The loss of wing vein phenotype caused by expression of HLHmβ^{Scer\UAS.cdCa} under the control of Scer\GAL4^Bx-MS1096 is enhanced by coexpression of emc^{Scer\UAS.cdCa}.

(Baonza et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescues

Scer\GAL4^Act.PU/emc^UAS.cdCa partially rescues emc^AP6/emc^Δ1

(Li and Baker, 2018)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

emc^{Scer\UAS.cdCa}

emc^UAS.cdCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of de Celis

(Gupta and Rodrigues, 1997)

Secondary FlyBase IDs

References (8)

Report Sections

Open Close