FlyBase Allele Report: Dmel\ptc[EP941]

General Information

Symbol

Dmel\ptc^EP941

Species

D. melanogaster

Name

FlyBase ID

FBal0088689

Feature type

allele

Associated gene

Dmel\ptc

Associated Insertion(s)

P{EP}ptc^EP941

Carried in Construct

Also Known As

EP(2)0941

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Rorth et al., 1998)

Progenitor genotype

Associated Insertion(s)

P{EP}ptc^EP941

Cytology

Description

P{EP} insertion 768bp upstream of the ptc translation initiation codon. The P{EP} element is oriented such that expression from the element should result in overexpression of ptc.

(Bidet et al., 2003)

Allele components

Component

Use(s)

Inserted element

P{EP}

misexpression element

Regulatory region(s)

UAS

binary expression system - regulatory region

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2R:8,649,617..8,649,617 [+]

(Bidet et al., 2003, Pena-Rangel et al., 2002, Gene Disruption Project members, 2001-, Chanut et al., 2000, Denef et al., 2000, Rorth et al., 1998)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible, with Scer\GAL4^Bx-MS1096

(Hooper, 2003)

visible | dominant, with Scer\GAL4^bi-omb-Gal4

(Rorth et al., 1998)

Phenotype Manifest In

wing, with Scer\GAL4^bi-omb-Gal4

(Rorth et al., 1998)

wing, with Scer\GAL4^Bx-MS1096

(Hooper, 2003)

wing vein L3, with Scer\GAL4^Bx-MS1096

(Hooper, 2003)

Detailed Description

Statement

Reference

Expression of ptc^EP941 under the control of Scer\GAL4^Bx-MS1096 results in a reduction in the size of the AP axis of the wing and abolishes the L3/4 territory and much of wing vein L3.

(Hooper, 2003)

Expression of ptc^EP941 under the control of Scer\GAL4^bi-omb-Gal4 produces wings of reduced size, particularly in the region between veins 3 and 4.

(Rorth et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

ptc^EP941/Scer\GAL4^pnr-MD237 is an enhancer of visible phenotype of pnr^MD237

(Pena-Rangel et al., 2002)

Suppressor of

Statement

Reference

ptc^EP941/Scer\GAL4^h-H10 is a suppressor of visible phenotype of ro^DOM

(Chanut et al., 2000)

Phenotype Manifest In

Suppressor of

Statement

Reference

ptc^EP941/Scer\GAL4^Bx-MS1096 is a suppressor of costal cell phenotype of Scer\GAL4^Bx-MS1096, smo^{C.UAS.Tag:MYC,Tag:Myr(v-Src)}

(Hooper, 2003)

ptc^EP941/Scer\GAL4^Bx-MS1096 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Bx-MS1096, smo^{C.UAS.Tag:MYC,Tag:Myr(v-Src)}

(Hooper, 2003)

ptc^EP941/Scer\GAL4^h-H10 is a suppressor of eye phenotype of ro^DOM

(Chanut et al., 2000)

Other

Statement

Reference

Scer\GAL4^Bx-MS1096, ptc^EP941, smo^{C.UAS.Tag:MYC,Tag:Myr(v-Src)} has wing phenotype

(Hooper, 2003)

Scer\GAL4^pnr-MD237, pnr^MD237, ptc^EP941 has scutellum phenotype

(Pena-Rangel et al., 2002)

Scer\GAL4^pnr-MD237, pnr^MD237/pnr[+], ptc^EP941 has scutellum phenotype

(Pena-Rangel et al., 2002)

Additional Comments

Genetic Interactions

Statement

Reference

Wings of flies co-expressing ptc^EP941 and high levels of smo^{Scer\UAS.cHa.T:Hsap\MYC} under the control of Scer\GAL4^Bx-MS1096 are indistinguishable from those of flies expressing ptc^EP941 alone. Co-expression of ptc^EP941 has no effect on the anterior overgrowth of the wing or ectopic venation caused by expression of high levels of fz::smo^FFS.Scer\UAS under the control of Scer\GAL4^Bx-MS1096, although ectopic venation is seen in a broad zone around the wing in these flies. Co-expression of ptc^EP941 completely suppresses the ectopic venation and costal overgrowth caused by expression of smo^{C.Scer\UAS.T:Hsap\MYC,T:Uuuu\Myr4} under the control of hemizygous Scer\GAL4^Bx-MS1096 and instead reduces the wing to a tiny scrap.

(Hooper, 2003)

pnr^MD237/+ adults expressing ptc^EP941 under the control of Scer\GAL4^pnr-MD237 do not form a scutellum.

(Pena-Rangel et al., 2002)

When expression is driven by Scer\GAL4^h-H10 in a ro^DOM mutant the stop-furrow phenotype of ro^DOM is substantially suppressed.

(Chanut et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Selected as: a P{EP} insertion line that modifies the pnr^MD237/+ phenotype when expressed using Scer\GAL4^pnr-MD237.

(Pena-Rangel et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

EP(2)0941^ptc

(Denef et al., 2000)

ptc^EP941

ptc[Ep941]

(Hooper, 2003)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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