FlyBase Allele Report: Dmel\ss[UAS.A5]

General Information

Symbol

Dmel\ss^UAS.A5

Species

D. melanogaster

Name

FlyBase ID

FBal0090105

Feature type

allele

Associated gene

Dmel\ss

Associated Insertion(s)

Carried in Construct

P{UAS-ss.A5}

Also Known As

UAS-ss

Key Links

Transgenic product class

cDNA

(Duncan et al., 1998)

wild_type

(Duncan et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Duncan et al., 1998)

wild_type

(Duncan et al., 1998)

Mutagen

in vitro construct

(Duncan et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-ss.A5}

Cytology

Description

Expression of ss cDNA sscA5 is governed by UAS regulatory sequences.

(Duncan et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

dendrite & dorsal multidendritic neuron ddaC, with Scer\GAL4^ppk.PG

(Kim et al., 2006)

dendrite & tracheal innervating neuron, with Scer\GAL4⁴⁷⁷

(Kim et al., 2006)

eye, with Scer\GAL4^GMR.long

(Wernet et al., 2006)

eye photoreceptor cell, with Scer\GAL4^GMR.long

(Wernet et al., 2006)

eye photoreceptor cell, with Scer\GAL4^GMR.PF

(Wernet et al., 2006)

eye photoreceptor cell | heat sensitive, with Scer\GAL4^hs.PB

(Wernet et al., 2006)

femur | distal

(Duncan et al., 1998)

leg | distal, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

leg anterior compartment

(Duncan et al., 1998)

maxillary palp, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

ommatidium, with Scer\GAL4^GMR.long

(Wernet et al., 2006)

photoreceptor cell R7 of pale ommatidium, with Scer\GAL4^GMR.PF

(Wernet et al., 2006)

photoreceptor cell R7 of pale ommatidium, with Scer\GAL4^Pan-R7

(Wernet et al., 2006)

photoreceptor cell R7 of pale ommatidium | heat sensitive, with Scer\GAL4^hs.PB

(Wernet et al., 2006)

photoreceptor cell R7 of yellow ommatidium | ectopic, with Scer\GAL4^GMR.PF

(Wernet et al., 2006)

photoreceptor cell R7 of yellow ommatidium | ectopic, with Scer\GAL4^Pan-R7

(Wernet et al., 2006)

photoreceptor cell R7 of yellow ommatidium | ectopic | heat sensitive, with Scer\GAL4^hs.PB

(Wernet et al., 2006)

rostral membrane, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

tergite, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

tibia, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

tracheal branch primordium, with Scer\GAL4^69B

(Duncan et al., 1998)

ventral denticle belt | medial, with Scer\GAL4^69B

(Duncan et al., 1998)

wing, with Scer\GAL4^ptc-559.1

(Duncan et al., 1998)

wing, with Scer\GAL4^sd-SG29.1

(Adachi-Yamada et al., 2005)

wing disc | cell autonomous | somatic clone, with Scer\GAL4^Act5C.PI

(Adachi-Yamada et al., 2005)

wing margin, with Scer\GAL4^NP7148

(Adachi-Yamada et al., 2005)

Detailed Description

Statement

Reference

Expression of ss^Scer\UAS.A5 under the control of Scer\GAL4⁴⁷⁷ causes ectopic dendritic branches to form in the td neuron (the td neuron normally extends bipolar dendrites ventrally and dorsally with little or no branching).

Expression of ss^Scer\UAS.A5 under the control of Scer\GAL4^ppk.PG causes a severe reduction in terminal branching of the dendrites in the ddaC neuron.

(Kim et al., 2006)

ss^Scer\UAS.A5; Scer\GAL4^GMR.PF have normal retinal morphology but marker analysis indicates, transformation of pale ommatidium type R7 cells to a yellow-type ommatidium R7 cell fate and an abnormal rhodopsin expression profile in other eye photoreceptor subtypes. Apart from this, specification of inner vs outer photorecptor types and R7 vs R8 appears normal.

ss^Scer\UAS.A5; Scer\GAL4^hs.PB animals given a 30 minute heat shock at mid-pupal stages have a range of eye phenotypes from ransformation of pale ommatidium type R7 cells to a yellow-type ommatidium R7 cell fate to an abnormal rhodopsin expression profile in other all eye photoreceptor subtypes. Heat shocks during larval or early pupal stage are lethal.

ss^Scer\UAS.A5; Scer\GAL4^Pan-R7 have normal retinal morphology but marker analysis indicates, transformation of pale ommatidium type R7 cells to a yellow-type ommatidium R7 cell fate. This effect is also seen if the eyes are also homozygous for ss^D115.7. In neither case is this phenotype accompanied by a shift of R8 cell fate from pale-type to yellow-type in the affected ommatidia.

ss^Scer\UAS.A5; Scer\GAL4^GMR.long flies have rough eyes and an abnormal rhodopsin expression profile is seen in all eye photoreceptor subtypes.

(Wernet et al., 2006)

When :ss^Scer\UAS.A5 is driven by Scer\GAL4^sd-SG29.1, none of the adult mutant flies develop wings. When :ss^Scer\UAS.A5 is driven by Scer\GAL4^sd-NP7148, adult mutant flies develop wings that show incisions into their wing margins. Overexpression of ss^Scer\UAS.A5 in clones in the wing (driven by Scer\GAL4^Act5C.PI) leads to apoptosis in which cell autonomy is position dependent. Clones have a rounded shape.

(Adachi-Yamada et al., 2005)

When expression is driven by Scer\GAL4^69B, embryonic trachea and midline are disorganised. Expression driven by Scer\GAL4^ptc-559.1 causes transformation of maxillary palp, rostral membrane and distal leg to antenna. Palps are also often deleted, as are medial leg structures. Anterior structures from the distal femur and most of the tibia are deleted. Much of posterior compartment remains, and produced irregular cuticular out growths. The distal basitarsus is retained and more distal tarsal segments are usually unaffected, with the exception of transformation of claw to arista. Central part of wing is deleted, a zone of polarity reversal occurs in the abdominal tergites and a few scattered bristles are induced in the wing.

(Duncan et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^NP7148, ss^UAS.A5 has wing margin phenotype, suppressible by Dll⁵/Dll[+]

(Adachi-Yamada et al., 2005)

Scer\GAL4^NP7148, ss^UAS.A5 has wing margin phenotype, suppressible by tgo^RNAi.UAS.cAa/Scer\GAL4^NP7148

(Adachi-Yamada et al., 2005)

Other

Statement

Reference

Diap1^UAS.Tag:MYC, Scer\GAL4^sd-SG29.1, ss^UAS.A5 has wing phenotype

(Adachi-Yamada et al., 2005)

Diap1^UAS.Tag:MYC, Scer\GAL4^sd-SG29.1, ss^UAS.A5 has pretarsus | ectopic phenotype

(Adachi-Yamada et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

When th^{Scer\UAS.T:Hsap\MYC} and ss^Scer\UAS.A5 are co-expressed under the control of Scer\GAL4^sd-SG29.1, an ectopic tarsus-like structure is is generated in the wing. When ss^Scer\UAS.A5 expressing clones are made in the wing disc, they retain a rounded shape, even after the addition of W⁰⁵⁰¹⁴ or hep^CA.

(Adachi-Yamada et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

ss^UAS.A5/Scer\GAL4²²¹ rescues ss^D115.7/ss¹³⁴

(Kim et al., 2006)

Comments

The primary branch overgrowth and increased terminal branching seen in ss¹³⁴/ss^D115.7 class I dendrites is rescued by expression of ss^Scer\UAS.A5 under the control of Scer\GAL4²²¹.

(Kim et al., 2006)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78354

w^*; P{UAS-ss.A5}A1/SM6a

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ss^Scer\UAS.A5

ss^UAS.A5

Name Synonyms

Secondary FlyBase IDs

References (13)

Report Sections

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