FlyBase Allele Report: Dmel\fz2[UAS.cCa]

General Information

Symbol

Dmel\fz2^UAS.cCa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Cadigan

FlyBase ID

FBal0090424

Feature type

allele

Associated gene

Dmel\fz2

Associated Insertion(s)

Carried in Construct

P{UAS-fz2.C}

Also Known As

UAS-DFz2, UAS-Fz2, UASDfz2

Key Links

Transgenic product class

wild_type

(Cadigan et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Cadigan et al., 1998)

Mutagen

in vitro construct

(Cadigan et al., 1998)

Progenitor genotype

Carried in construct

P{UAS-fz2.C}

Cytology

Description

A 2.2kb fragment of the fz2 coding region is expressed under the control of UAS regulatory sequences.

(Cadigan et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

fz2

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of pupal stage | heat sensitive, with Scer\GAL4^h-1J3

(Cadigan et al., 1998)

some die during pupal stage | heat sensitive, with Scer\GAL4^h-1J3

(Cadigan et al., 1998)

visible, with Scer\GAL4^69B

(Lin and Perrimon, 1999, Cadigan et al., 1998)

visible, with Scer\GAL4^71B

(Cadigan et al., 1998)

visible, with Scer\GAL4^bbg-C96

(Baeg et al., 2004)

visible, with Scer\GAL4^Bx-MS1096

(Wehrli et al., 2000)

visible, with Scer\GAL4^h-1J3

(Cadigan et al., 1998)

Phenotype Manifest In

bouton, with Scer\GAL4^C57

(Packard et al., 2002)

chaeta | ectopic & wing, with Scer\GAL4^bbg-C96

(Baeg et al., 2004)

dorsal denticle, with Scer\GAL4^da.G32

(Buratovich et al., 2000)

embryonic epidermis | dorsal, with Scer\GAL4^da.G32

(Buratovich et al., 2000)

macrochaeta | ectopic, with Scer\GAL4^69B

(Lin and Perrimon, 1999)

mitochondrion & bouton, with Scer\GAL4^C57

(Packard et al., 2002)

neuromuscular junction, with Scer\GAL4^C57

(Packard et al., 2002)

postsynaptic membrane, with Scer\GAL4^C57

(Packard et al., 2002)

stalk follicle cell | increased number | heat sensitive, with Scer\GAL4^hs.PB

(Song and Xie, 2003)

t-bar, with Scer\GAL4^C57

(Packard et al., 2002)

wing, with Scer\GAL4^69B

(Lin and Perrimon, 1999, Cadigan et al., 1998)

wing, with Scer\GAL4^71B

(Cadigan et al., 1998)

wing, with Scer\GAL4^bbg-C96

(Baeg et al., 2004)

wing, with Scer\GAL4^Bx-MS1096

(Wehrli et al., 2000)

wing, with Scer\GAL4^h-1J3

(Cadigan et al., 1998)

wing & macrochaeta, with Scer\GAL4^69B

(Lin and Perrimon, 1999)

wing margin bristle | ectopic, with Scer\GAL4^Bx-MS1096

(Wehrli et al., 2000)

wing sensillum | ectopic, with Scer\GAL4^69B

(Cadigan et al., 1998)

wing sensillum | ectopic, with Scer\GAL4^71B

(Cadigan et al., 1998)

wing sensillum | ectopic, with Scer\GAL4^h-1J3

(Cadigan et al., 1998)

Detailed Description

Statement

Reference

When fz2^Scer\UAS.cCa is driven by Scer\GAL4^C96 mutant wings have notched wings and ectopic bristles near the wing margin.

(Baeg et al., 2004)

Expression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^hs.PB using four days of pulsed heat shock treatment consistently produces more follicle cells that accumulate between egg chambers. These extra follicle cells form long stalks with multiple rows of cells instead of one row in the wild type.

(Song and Xie, 2003)

When fz2^Scer\UAS.cCa is driven by Scer\GAL4^C57 synaptic boutons lack Subsynaptic reticulum, active zones and mitochondria.

(Packard et al., 2002)

Expression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^da.G32 results in a mild ectopic wg phenotype in the dorsal epidermis of the embryo, consisting of expanded 4^o cells at the expense of 3^o cells (in wild-type embryos each parasegment contains 4 types of cells in the dorsal epidermis; the 1^o, 2^o and 3^o cells in the anterior half of the parasegment and the 4^o cells in the posterior half of the parasegment).

(Buratovich et al., 2000)

Overexpression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^Bx-MS1096 results in ectopic margin bristles in the wing.

(Wehrli et al., 2000)

Uniform expression of fz2^Scer\UAS.cCa in the wing pouch by Scer\GAL4^69B leads to wings with ectopic bristles.

(Lin and Perrimon, 1999)

Ubiquitous overexpression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^e22c does not alter epidermal patterning in a wild-type background.

(Moline et al., 1999)

Animals expressing fz2^Scer\UAS.cCa under the control of Scer\GAL4^h-1J3 usually die as pupae at 29^oC. At lower temperatures, some escapers are seen. These adults have ectopic bristles on the wing blades. Ectopic bristles in the anterior compartment are almost always of the slender or chemosensory type, although an occasional stout bristle is also seen. Ectopic bristles in the posterior compartment are similar to the noninnervated bristles seen at the posterior margin. This "hairy wing" phenotype is also seen in flies expressing fz2^Scer\UAS.cCa under the control of Scer\GAL4^69B or Scer\GAL4^71B.

(Cadigan et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^69B, fz2^UAS.cCa has visible phenotype, suppressible by dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

NOT Suppressor of

Statement

Reference

fz2^UAS.cCa/Scer\GAL4^Bx-MS1096 is a non-suppressor of visible | recessive | somatic clone phenotype of arr²

(Wehrli et al., 2000)

Other

Statement

Reference

Scer\GAL4^30A, fz2^UAS.cCa, wg^l-12.UAS has visible phenotype

(Buratovich and Wilder, 2001)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^69B, fz2^UAS.cCa has wing phenotype, suppressible by dally⁰⁶⁴⁶⁴

(Lin and Perrimon, 1999)

NOT Suppressor of

Statement

Reference

Scer\GAL4^prd.RG1/fz2^UAS.cCa is a non-suppressor of ventral denticle belt | ectopic phenotype of wg^l-17

(Tolwinski et al., 2003)

Scer\GAL4^prd.RG1/fz2^UAS.cCa is a non-suppressor of embryonic/first instar larval cuticle phenotype of wg^l-17

(Tolwinski et al., 2003)

fz2^UAS.cCa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing margin bristle | somatic clone phenotype of arr²

(Wehrli et al., 2000)

fz2^UAS.cCa/Scer\GAL4^Bx-MS1096 is a non-suppressor of wing | somatic clone phenotype of arr²

(Wehrli et al., 2000)

Other

Statement

Reference

Scer\GAL4^30A, fz2^UAS.cCa, wg^l-12.UAS has 3rd axillary sclerite phenotype

(Buratovich and Wilder, 2001)

Scer\GAL4^e22c, fz2^UAS.cCa, wg[+]/wg^l-17 has ventral denticle belt phenotype

(Moline et al., 1999)

Scer\GAL4^e22c, fz2^UAS.cCa, wg[+]/wg^l-17 has embryonic/first instar larval cuticle phenotype

(Moline et al., 1999)

Scer\GAL4^e22c, fz2^UAS.cCa, wg^l-17 has ventral denticle belt phenotype

(Moline et al., 1999)

Scer\GAL4^e22c, fz2^UAS.cCa, wg^l-17 has embryonic/first instar larval cuticle phenotype

(Moline et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

Smooth cuticle formation in wg^l-17/wg^l-17 embryos is not restored by fz2^Scer\UAS.cCa; Scer\GAL4^prd.RG1.

(Tolwinski et al., 2003)

Coexpression of wg^{l-12.Scer\UAS} and fz2^Scer\UAS.cCa under the control of Scer\GAL4^30A results in a diminution of axillary sclerite 3 in the wing hinge.

(Buratovich and Wilder, 2001)

The notching and loss of margin bristles seen in arr² clones in the wing is not suppressed if fz2^Scer\UAS.cCa is expressed in these flies under the control of Scer\GAL4^Bx-MS1096.

(Wehrli et al., 2000)

The ectopic bristles produced by expression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^69B are strikingly reduced in fz2^Scer\UAS.cCa, dally⁰⁶⁴⁶⁴/Scer\GAL4^69B, dally⁰⁶⁴⁶⁴ flies.

(Lin and Perrimon, 1999)

Overexpression of fz2^Scer\UAS.cCa under the control of Scer\GAL4^e22c in a wg^l-17/+ background results in cuticle patterning defects in 60% of embryos; ectopic denticles appear in the domain of cells that normally secrete naked cuticle.

(Moline et al., 1999)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

fz2^UAS.cCa/Scer\GAL4^da.G32 rescues Df(3L)fz2

(Bhanot et al., 1999)

Partially rescues

fz2^UAS.cCa/Scer\GAL4^arm.PS partially rescues Df(3L)N2-27

(Bhat, 1998)

Comments

Rescues the embryonic cuticle phenotype of double mutants of fz²¹ or fz²³ with Df(3L)fz2.

(Bhanot et al., 1999)

The RP2/sib lineage defects seen in fz2 deficient Df(3L)N2-27 embryos are partially rescued by fz2^Scer\UAS.cCa expressed under the control of Scer\GAL4^arm.PS.

(Bhat, 1998)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

fz2^Scer\UAS.cCa

fz2^UAS.cCa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Cadigan

(Cadigan et al., 1998)

Secondary FlyBase IDs

References (13)

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