FlyBase Allele Report: Dmel\Cdc42[GMR.PN]

General Information

Symbol

Dmel\Cdc42^GMR.PN

Species

D. melanogaster

Name

glass multimer reporter construct of Nolan

FlyBase ID

FBal0094048

Feature type

allele

Associated gene

Dmel\Cdc42

Associated Insertion(s)

Carried in Construct

P{GMR-Cdc42.N}

Also Known As

GMR-Cdc42

Key Links

Transgenic product class

wild_type

(Nolan et al., 1998)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

wild_type

(Nolan et al., 1998)

Mutagen

in vitro construct

(Nolan et al., 1998)

Progenitor genotype

Carried in construct

P{GMR-Cdc42.N}

Cytology

Description

Full length coding region is expressed under the control of GMR regulatory sequences.

(Nolan et al., 1998)

Allele components

Component

Use(s)

Regulatory region(s)

GMR

engineered transcription regulatory region

Encoded product / tool

Cdc42

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

visible

(Avet-Rochex et al., 2005, Raymond et al., 2001, Nolan et al., 1998)

Phenotype Manifest In

eye

(Tan et al., 2003, Raymond et al., 2001, Nolan et al., 1998)

interommatidial bristle

(Avet-Rochex et al., 2005)

ommatidium

(Avet-Rochex et al., 2005, Raymond et al., 2001, Nolan et al., 1998)

photoreceptor

(Nolan et al., 1998)

pigment cell

(Nolan et al., 1998)

rhabdomere

(Nolan et al., 1998)

Detailed Description

Statement

Reference

Flies expressing Cdc42^GMR.PN have slight eye defects associated with occasional fusion of ommatidial units and misoriented bristles.

(Avet-Rochex et al., 2005)

Cdc42^GMR.PN flies have rough eyes.

(Tan et al., 2003)

Flies carrying Cdc42^GMR.PN have moderately reduced rough eyes with an almost normal array of well-defined ommatidia.

(Raymond et al., 2001)

Expression of Cdc42^GMR.PN in a wild-type background does not disrupt photoreceptor axon projections.

(Newsome et al., 2000)

Flies bearing one copy of P{GMR-Cdc42.N} show a rough eye phenotype distinct from that caused by P{GMR-Rac1.N} or P{GMR-Rac2.N}. Ommatidial morphology is disrupted and photoreceptor cells are occasionally lost. Rhabdomere morphology is abnormal. Postmitotic elongation that establishes the depth of the retina is aberrant.

(Nolan et al., 1998)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Cdc42^GMR.PN has visible phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Other

Statement

Reference

Cdc42^GMR.PN, Df(3R)rn20/+ has visible phenotype

(Raymond et al., 2001)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Cdc42^GMR.PN has eye phenotype, non-enhanceable by Mbs[+]/Mbs⁰³⁸⁰²

(Tan et al., 2003)

Suppressed by

Statement

Reference

Cdc42^GMR.PN has ommatidium phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Cdc42^GMR.PN has interommatidial bristle phenotype, suppressible by Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

NOT suppressed by

Statement

Reference

Cdc42^GMR.PN has eye phenotype, non-suppressible by Mbs[+]/Mbs⁰³⁸⁰²

(Tan et al., 2003)

Cdc42^GMR.PN has eye phenotype, non-suppressible by mbc^1.63

(Nolan et al., 1998)

Cdc42^GMR.PN has eye phenotype, non-suppressible by mbc^2.35

(Nolan et al., 1998)

Cdc42^GMR.PN has eye phenotype, non-suppressible by mbc^3.5

(Nolan et al., 1998)

Cdc42^GMR.PN has eye phenotype, non-suppressible by mbc^4.25

(Nolan et al., 1998)

NOT Enhancer of

Statement

Reference

Cdc42^GMR.PN is a non-enhancer of phenotype of trio^{GEF1.GMR.Tag:MYC}

(Newsome et al., 2000)

NOT Suppressor of

Statement

Reference

Cdc42^GMR.PN is a non-suppressor of phenotype of trio^{GEF1.GMR.Tag:MYC}

(Newsome et al., 2000)

Other

Statement

Reference

Cdc42^GMR.PN, Df(3R)rn20/+ has eye phenotype

(Raymond et al., 2001)

Cdc42^GMR.PN, Df(3R)rn20/+ has ommatidium phenotype

(Raymond et al., 2001)

Cdc42^GMR.PN, Df(3R)rn20/+ has interommatidial bristle phenotype

(Raymond et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype of Cdc42^GMR.PN flies in unaffected by heterozygosity for Mbs⁰³⁸⁰².

(Tan et al., 2003)

Flies carrying Cdc42^GMR.PN in a Df(3R)rn20/+ background have larger and less rough eyes than flies carrying Cdc42^GMR.PN in a wild-type background, but ommatidial packing is affected more in Cdc42^GMR.PN Df(3R)rn20/+ flies; the ommatidia are less closely associated with each other, resulting in gaps or channels between them. Interommatidial bristle are sometimes duplicated.

(Raymond et al., 2001)

Does not significantly alter the photoreceptor axon projection defects caused by expression of trio^{GEF1.GMR.T:Hsap\MYC}.

(Newsome et al., 2000)

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF restores the eye morphology to wild type in Cdc42^GMR.PN flies.

(Avet-Rochex et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

Cdc42^GMR.PN

Name Synonyms

glass multimer reporter construct of Nolan

(Nolan et al., 1998)

Secondary FlyBase IDs

References (6)

Report Sections

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