FlyBase Allele Report: Paer\ExoS[GAP.UAS]

General Information

Symbol

Paer\ExoS^GAP.UAS

Species

P. aeruginosa

Name

FlyBase ID

FBal0321127

Feature type

allele

Associated gene

Paer\ExoS

Associated Insertion(s)

Carried in Construct

P{UAS-ExoSGAP}

Transgenic product class

inframe_deletion

(Avet-Rochex et al., 2005)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Avet-Rochex et al., 2005)

Mutagen

in vitro construct

(Avet-Rochex et al., 2005)

Progenitor genotype

Carried in construct

P{UAS-ExoSGAP}

Cytology

Description

UAS regulatory sequences drive expression of the Paer\ExoS GTPase activating (GAP) domain.

(Avet-Rochex et al., 2005)

Allele components

Component

Use(s)

Regulatory region(s)

UAS

binary expression system - regulatory region

Encoded product / tool

Paer\ExoS

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval plasmatocyte, with Scer\GAL4^crq.PU

(Avet-Rochex et al., 2005)

embryonic/larval plasmatocyte, with Scer\GAL4^srp.D.cCa

(Avet-Rochex et al., 2005)

eye, with Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

interommatidial bristle, with Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

ommatidium, with Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

wing | posterior, with Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

wing vein | ectopic, with Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Detailed Description

Statement

Reference

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF have slightly rough eyes.

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^en-e16E have a few additional veins in the posterior part of the wing.

(Avet-Rochex et al., 2007)

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF have slight defects in ommatidial organisation with occasional loss of misoriented bristles.

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of either Scer\GAL4^srp.D.cCa or Scer\GAL4^hs.PB show reduced resistance to infection by P.aeruginosa (both the cytotoxic CHA strain and the mutant CHA::exsA strain) (infection by septic injury).

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^yolk show wild-type levels of resistance to infection by P.aeruginosa(both the cytotoxic CHA strain and the mutant CHA::exsA strain) (infection by septic injury).

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of either Scer\GAL4^srp.D.cCa or Scer\GAL4^da.G32 show reduced resistance to oral infection by the P.aeruginosa CHA strain.

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^srp.D.cCa show reduced phagocytosis of injected bacteria compared to control flies (when assayed 40 minutes after injection).

Flies expressing Zzzz\ExoS^GAP.Scer\UAS under the control of either Scer\GAL4^srp.D.cCa or Scer\GAL4^da.G32 show reduced resistance to infection by S.aureus (infection by septic injury).

Circulating plasmatocytes from third instar larvae expressing Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^srp.D.cCa or Scer\GAL4^crq.PU show a reduced phagocytic index (for dead E.coli and dead S.aureus) compared to control plasmatocytes.

(Avet-Rochex et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Rac2^UAS.cARa, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

Paer\ExoS^GAP.UAS, Scer\GAL4^da.G32 is a suppressor of abnormal immune response phenotype of Rac2^UAS.cARa, Scer\GAL4^da.G32

(Avet-Rochex et al., 2007)

Paer\ExoS^GAP.UAS, Scer\GAL4^en-e16E is a suppressor of visible phenotype of Rac2^UAS.cARa, Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of visible phenotype of Rac1^UAS.cLa, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Cdc42^GMR.PN

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of visible phenotype of Rho1^GMR.PH

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of lethal | pupal stage phenotype of Rac1^UAS.cLa, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Phenotype Manifest In

Suppressor of

Statement

Reference

Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF is a suppressor of eye phenotype of Rac2^UAS.cARa, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2007)

Paer\ExoS^GAP.UAS, Scer\GAL4^en-e16E is a suppressor of wing | posterior phenotype of Rac2^UAS.cARa, Scer\GAL4^en-e16E

(Avet-Rochex et al., 2007)

Paer\ExoS^GAP.UAS, Scer\GAL4^GMR.PF is a suppressor | partially of eye phenotype of Rac1^UAS.cLa, Scer\GAL4^GMR.PF

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Rho1^GMR.PH

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Rho1^GMR.PH

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of ommatidium phenotype of Cdc42^GMR.PN

(Avet-Rochex et al., 2005)

Paer\ExoS^GAP.UAS/Scer\GAL4^GMR.PF is a suppressor of interommatidial bristle phenotype of Cdc42^GMR.PN

(Avet-Rochex et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Co-expression of Zzzz\ExoS^GAP.Scer\UAS suppresses the rough eye phenotype caused by expression of Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^GMR.PF.

Co-expression of Zzzz\ExoS^GAP.Scer\UAS suppresses the wing phenotype caused by expression of Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^en-e16E.

The increased sensitivity to P.aeruginosa following oral infection that is seen in flies expressing Rac2^{Scer\UAS.cARa} under the control of Scer\GAL4^da.G32 is suppressed by co-expression of Zzzz\ExoS^GAP.Scer\UAS.

(Avet-Rochex et al., 2007)

The viability of flies expressing Rac1^Scer\UAS.cLa under the control of Scer\GAL4^GMR.PF is enhanced to 50% by co-expression of Zzzz\ExoS^GAP.Scer\UAS. In addition, the eye morphology of the surviving adults is restored towards wild type.

Co-expression of Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF restores the eye morphology to wild type in Cdc42^GMR.PN flies.

Co-expression of Zzzz\ExoS^GAP.Scer\UAS under the control of Scer\GAL4^GMR.PF restores the eye morphology to wild type in Rho1^GMR.PH flies.

(Avet-Rochex et al., 2005)

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

26649

w¹¹¹⁸; P{UAS-ExoSGAP}31.7

26648

w¹¹¹⁸; P{UAS-ExoSGAP}31.8/CyO, P{GAL4-twi.G}2.2, P{UAS-2xEGFP}AH2.2

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

Paer\ExoS^GAP.Scer\UAS

Paer\ExoS^GAP.UAS

Zzzz\ExoS^GAP.Scer\UAS

Name Synonyms

Secondary FlyBase IDs

FBal0320513
FBal0219715

References (3)

Report Sections

Open Close