FlyBase Allele Report: Dmel\dup[a1]

General Information

Symbol

Dmel\dup^a1

Species

D. melanogaster

Name

FlyBase ID

FBal0095191

Feature type

allele

Associated gene

Dmel\dup

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Cytology

Description

Amino acid replacement: K171term.

(Whittaker et al., 2000)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2R:15,379,792..15,379,792 [-]

Nucleotide change:

A15379792T

Amino acid change:

K171term | dup-PA; K171term | dup-PB

Reported amino acid change:

K171term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Whittaker et al., 2000)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cytokinesis

(Pandey et al., 2005)

abnormal mitotic cell cycle

(Pandey et al., 2005, Lee et al., 2004, Garner et al., 2001)

decreased occurrence of cell division | embryonic stage

(Sloan et al., 2012)

decreased occurrence of cell division | embryonic stage | maternal effect

(Shibutani et al., 2007)

lethal - all die during embryonic stage | recessive

(Whittaker, 1999.3.27)

some die during embryonic stage | recessive

(Whittaker, 1999.3.27)

Phenotype Manifest In

follicle cell

(Whittaker et al., 2000)

follicle cell (with dup^PA77)

(Whittaker et al., 2000)

metaphase plate

(Pandey et al., 2005)

mitosis & nuclear chromosome

(Garner et al., 2001)

mitotic cell cycle

(Garner et al., 2001, Whittaker et al., 2000)

mitotic cell cycle (with dup^PA77)

(Whittaker et al., 2000)

mitotic domain 1 | embryonic cycle 16

(Shibutani et al., 2007, Dej et al., 2004)

mitotic metaphase

(Garner et al., 2001)

nurse cell

(Whittaker et al., 2000)

nurse cell (with dup^PA77)

(Whittaker et al., 2000)

S phase

(Whittaker et al., 2000)

S phase | embryonic cycle 16

(Lee et al., 2004, Quinn et al., 2001)

Detailed Description

Statement

Reference

Homozygous embryos show no BrdU incorporation in cycle 16 nuclei.

(Sloan et al., 2012)

S phase 16 does not occur in homozygous embryos.

(Shibutani et al., 2007)

dup^a1 mutants exhibit an increase in mitotic index. Chromosomes fail to congress into a metaphase plate during mitosis 16.

(Pandey et al., 2005)

Mutant embryos fail to replicate chromosome in the S phase prior to mitosis 16, resulting in mitotic figures in mitosis 16 that contain single chromatids that are fully condensed.

(Dej et al., 2004)

S phase of cycle 16 is blocked in mutant embryos and cells enter mitosis as unreplicated nuclei, with chromosomes often appearing overcondensed and fragmented.

(Lee et al., 2004)

Cells in homozygous dup^a1 mutant embryos enter mitosis 16 at the same time as heterozygous controls. But S phase 16 is absent. Once in M16 the cells are arrested and remain in this state until at least stage 11 and mitotic cells are still visible at stage 15. Spindles in these mutants contain apparently functional centrosomes, chromosomes fail to align properly. Most chromosomes lie within the bipolar spindle but are scattered and not compacted into a metaphase plate. Severe alignment defects are seen in about 84% of mitotic cells.

(Garner et al., 2001)

DNA replication is blocked in S phase of cycle 16 in homozygous dup^a1 embryos borne of heterozygous mothers. Reduced levels of chorion amplification are also seen. In ovaries from dup^PA77/dup^a1 female flies, incorporation of BrdU into the follicle cell genome is reduced. This reduction is most apparent at stage 9. No incorporation is seen at the chorion loci at any stage.

(Whittaker et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

dup^a1 has abnormal mitotic cell cycle phenotype, suppressible by pim^IL

(Pandey et al., 2005)

dup^a1 has abnormal cytokinesis phenotype, suppressible by pim^IL

(Pandey et al., 2005)

Enhancer of

Statement

Reference

dup[+]/dup^a1 is an enhancer of visible phenotype of Scer\GAL4^GMR.PF, geminin^UAS.cQa

(Quinn et al., 2001)

Suppressor of

Statement

Reference

dup^a1 is a suppressor of abnormal cytokinesis phenotype of pim^IL

(Pandey et al., 2005)

dup^a1 is a suppressor of abnormal mitotic cell cycle phenotype of pim^IL

(Pandey et al., 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

dup^a1 has metaphase plate phenotype, suppressible by pim^IL

(Pandey et al., 2005)

dup^a1 has S phase | embryonic cycle 16 phenotype, suppressible by geminin^unspecified

(Quinn et al., 2001)

Enhancer of

Statement

Reference

dup[+]/dup^a1 is an enhancer of eye phenotype of Scer\GAL4^GMR.PF, geminin^UAS.cQa

(Quinn et al., 2001)

dup[+]/dup^a1 is an enhancer of ommatidium phenotype of Scer\GAL4^GMR.PF, geminin^UAS.cQa

(Quinn et al., 2001)

Suppressor of

Statement

Reference

dup^a1 is a suppressor of metaphase plate phenotype of pim^IL

(Pandey et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

In contrast to pim^IL and dup^a1 single mutants, double mutants do not exhibit an increase in mitotic index. Chromosomes are found to congress into a metaphase plate during embryonic mitosis 16 as in wild-type.

(Pandey et al., 2005)

In dup^a1 Cap-G^K4 double mutants, cells at cycle 16 contain unreplicated chromosomes that fail to condense into discernible metaphase chromosomes.

(Dej et al., 2004)

dup^a1/+ enhances the rough eye phenotype caused by expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^GMR.PF. The dup^a1 embryonic cycle 16 S phase defect is suppressed by geminin^unspecified.

(Quinn et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

dup^PA77

(Whittaker, 1999.3.27)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

7275

dup^a1/CyO

Notes on Origin

Discoverer

Comments

dup^a1 fails to complement dup^l5.

(Whittaker, 1999.3.27)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

(Whittaker, 1999.3.27)

dup^a1

(Sloan et al., 2012, Shibutani et al., 2007, Pandey et al., 2005)

dup^α1

(Quinn et al., 2001)

speckled^a1

Name Synonyms

Secondary FlyBase IDs

References (11)

Report Sections

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