FlyBase Allele Report: Dmel\geminin[UAS.cQa]

General Information

Symbol

Dmel\geminin^UAS.cQa

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Quinn

FlyBase ID

FBal0127984

Feature type

allele

Associated gene

Dmel\geminin

Associated Insertion(s)

Carried in Construct

P{UAS-geminin.Q}

Also Known As

UAS-Geminin, UAS-Gem

Key Links

Transgenic product class

cDNA

(Quinn et al., 2001)

wild_type

(Quinn et al., 2001)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

cDNA

(Quinn et al., 2001)

wild_type

(Quinn et al., 2001)

Mutagen

in vitro construct

(Quinn et al., 2001)

Progenitor genotype

Carried in construct

P{UAS-geminin.Q}

Cytology

Description

UASt regulatory sequences drive expression of a geminin cDNA (EST LD07974).

(Quinn et al., 2001)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

geminin

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

embryonic/larval neuron | ectopic, with Scer\GAL4^en-e16E

(Quinn et al., 2001)

eye, with Scer\GAL4^ey.PH

(Quinn et al., 2001)

eye, with Scer\GAL4^GMR.PF

(Quinn et al., 2001)

eye disc, with Scer\GAL4^ey.PH

(Quinn et al., 2001)

eye disc, with Scer\GAL4^GMR.PF

(Quinn et al., 2001)

larval abdominal lch5 neuron, with Scer\GAL4^h-1J3

(Herr et al., 2010)

mitotic M phase, with Scer\GAL4^en-e16E

(Quinn et al., 2001)

ommatidium, with Scer\GAL4^GMR.PF

(Quinn et al., 2001)

peripheral nervous system | ectopic, with Scer\GAL4^en-e16E

(Herr et al., 2010)

peripheral nervous system | ectopic, with Scer\GAL4^h-1J3

(Herr et al., 2010)

presumptive embryonic/larval peripheral nervous system, with Scer\GAL4^en-e16E

(Quinn et al., 2001)

S phase, with Scer\GAL4^en-e16E

(Quinn et al., 2001)

S phase, with Scer\GAL4^ey.PH

(Quinn et al., 2001)

S phase, with Scer\GAL4^GMR.PF

(Quinn et al., 2001)

wing disc | third instar larval stage, with Scer\GAL4^hs.PB

(Herr et al., 2010)

Detailed Description

Statement

Reference

Expression of geminin^Scer\UAS.cQa via the Scer\GAL4^en-e16E driver produces adult flies with relatively normal wings compared with wild-type flies.

Expression of geminin^Scer\UAS.cQa driven by Scer\GAL4^en-e16E results in ectopic peripheral nervous system (PNS) cells.

Expression of geminin^Scer\UAS.cQa driven by Scer\GAL4^h-1J3 results in ectopic PNS cells. There is also a loss of neural cells in the PNS, particularly in the lateral chordotonal clusters (lch5).

Expression of geminin^Scer\UAS.cQa driven by Scer\GAL4^hs.PB results in a slight reduction of S-phase cells in third instar imaginal wing discs compared with controls. geminin^Scer\UAS.cQa expression also results in a slight increase in the number of mitotic figures and the rate of cell death compared with controls.

(Herr et al., 2010)

Expression of geminin^Scer\UAS.cQa in 'flip out' clones in salivary glands blocks DNA replication.

(Narbonne-Reveau et al., 2008)

Expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^neur-P72 at 30[o]C at 0 hours after pupal formation abolishes S-phase in the bristle lineage (no BrdU incorporation is seen at 18 hours after pupal formation in contrast to controls).

(Remaud et al., 2008)

Expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^hs.PB during embryogenesis (using heat shock) results in a general decrease in BrdU-labeling cells in mitotic and endoreplicating tissues. Expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^en-e16E results in a dramatic decrease in S phase cells within the stripe of geminin-expressing cells compared to the surrounding cells. 4 times as many as normal of the geminin-expressing cells are in mitosis. Stage 11 embryos show many apoptotic cells in the geminin-expressing stripes (in contrast to wild type). These embryos have ectopic neuronal cells in a position more epidermal than normal PNS neurons. Some disruption of the normal PNS pattern is observed. Expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^ey.PH results in a dramatic decrease in S phases and the size of the 3rd larval instar eye disc and a reduction in size of the adult eye. Expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^GMR.PF results in a 40-50% decrease in S phase cells in the region posterior to the morphogenetic furrow in 3rd larval instar eye discs and a severely rough eye in the adult.

(Quinn et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, geminin^UAS.cQa has visible phenotype, enhanceable by dup[+]/dup^a1

(Quinn et al., 2001)

NOT Enhanced by

Statement

Reference

Scer\GAL4^hs.PB, geminin^UAS.cQa has abnormal mitotic cell cycle | third instar larval stage phenotype, non-enhanceable by brm^{K804R.UAS.Tag:HA}/Scer\GAL4^hs.PB

(Herr et al., 2010)

NOT Enhancer of

Statement

Reference

geminin^UAS.cQa, Scer\GAL4^bbg-C96 is a non-enhancer of visible phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/geminin^UAS.cQa is a suppressor | partially of visible phenotype of Scer\GAL4^en-e16E, osa^UAS.cCa

(Herr et al., 2010)

geminin^UAS.cQa/brm^{K804R.UAS.Tag:HA}, Scer\GAL4^bbg-C96 is a suppressor | partially of visible phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

NOT Suppressor of

Statement

Reference

geminin^UAS.cQa, Scer\GAL4^bbg-C96 is a non-suppressor of visible phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Other

Statement

Reference

Scer\GAL4^en-e16E, Snr1^cdel.3.UAS, geminin^UAS.cQa has visible phenotype

(Herr et al., 2010)

Scer\GAL4^en-e16E, brm^{K804R.UAS.Tag:HA}, geminin^UAS.cQa has lethal | first instar larval stage phenotype

(Herr et al., 2010)

Phenotype Manifest In

Enhanced by

Statement

Reference

Scer\GAL4^GMR.PF, geminin^UAS.cQa has eye phenotype, enhanceable by dup[+]/dup^a1

(Quinn et al., 2001)

Scer\GAL4^GMR.PF, geminin^UAS.cQa has ommatidium phenotype, enhanceable by dup[+]/dup^a1

(Quinn et al., 2001)

NOT Enhancer of

Statement

Reference

geminin^UAS.cQa, Scer\GAL4^bbg-C96 is a non-enhancer of wing margin phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Suppressor of

Statement

Reference

Scer\GAL4^en-e16E/geminin^UAS.cQa is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4^en-e16E, osa^UAS.cCa

(Herr et al., 2010)

Scer\GAL4^en-e16E/geminin^UAS.cQa is a suppressor | partially of wing phenotype of Scer\GAL4^en-e16E, osa^UAS.cCa

(Herr et al., 2010)

geminin^UAS.cQa/brm^{K804R.UAS.Tag:HA}, Scer\GAL4^bbg-C96 is a suppressor | partially of wing margin phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

NOT Suppressor of

Statement

Reference

geminin^UAS.cQa, Scer\GAL4^bbg-C96 is a non-suppressor of wing margin phenotype of Raf^UAS.F179, Scer\GAL4^bbg-C96

(Herr et al., 2010)

Other

Statement

Reference

Scer\GAL4^en-e16E, Snr1^cdel.3.UAS, geminin^UAS.cQa has wing vein L5 phenotype

(Herr et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Co-expression of brm^{K804R.Scer\UAS.T:Ivir\HA1} and geminin^Scer\UAS.cQa driven by Scer\GAL4^en-e16E causes a dramatic developmental defect leading to lethality during the first instar larval stage.

Co-expression of Snr1^{cdel.3.Scer\UAS} and geminin^Scer\UAS.cQa driven by Scer\GAL4^en-e16E causes a shortening of wing vein L5.

Co-expression of geminin^Scer\UAS.cQa driven by Scer\GAL4^en-e16E strongly suppresses the osa^Scer\UAS.cCa wing phenotype, although some blistering is still detectable.

Co-expression of brm^{K804R.Scer\UAS.T:Ivir\HA1} driven by Scer\GAL4^hs.PB does not enhance the S-phase, mitotic and cell death phenotypes of geminin^Scer\UAS.cQa expression.

Co-expression of geminin^Scer\UAS.cQa with phl^{Scer\UAS.F179} via Scer\GAL4^bbg-C96 does not significantly affect the notched wing phenotype resulting from the expression of phl^{Scer\UAS.F179} alone.

Co-expression of both brm^{K804R.Scer\UAS.T:Ivir\HA1} and geminin^Scer\UAS.cQa with phl^{Scer\UAS.F179} via Scer\GAL4^bbg-C96 results in a significant suppression of the notched wing phenotype resulting from the expression of phl^{Scer\UAS.F179} alone.

(Herr et al., 2010)

dup^a1/+ enhances the rough eye phenotype caused by expression of geminin^Scer\UAS.cQa under the control of Scer\GAL4^GMR.PF.

(Quinn et al., 2001)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

56760

w^*; P{UAS-geminin.Q}3

59066

w^*; P{UAS-geminin.Q}43

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (3)

Reported As

Symbol Synonym

geminin^Scer\UAS.cQa

geminin^UAS.cQa

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Quinn

(Quinn et al., 2001)

Secondary FlyBase IDs

References (4)

Report Sections

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