FlyBase Allele Report: Dmel\brk[XH]

General Information

Symbol

Dmel\brk^XH

Species

D. melanogaster

Name

FlyBase ID

FBal0095491

Feature type

allele

Associated gene

Dmel\brk

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

loss of function allele

Mutagen

Delta2-3 transposase

Nature of the Allele

Allele class

loss of function allele

(Campbell and Tomlinson, 1999)

Mutagen

Delta2-3 transposase

(Campbell and Tomlinson, 1999)

Progenitor genotype

P{PZ}X47

(Campbell and Tomlinson, 1999)

Cytology

Description

Deletion extending from the site of insertion of P{PZ}X47 into the brk transcription unit removing the transcription and translation start sites.

(Campbell and Tomlinson, 1999)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die during embryonic stage | recessive

(Campbell and Tomlinson, 1999)

some die during embryonic stage | recessive

(Campbell and Tomlinson, 1999)

Phenotype Manifest In

abdominal 1 ventral denticle belt

(Torres-Vazquez et al., 2001)

dorsal ectoderm

(Torres-Vazquez et al., 2001)

embryonic ventral epidermis

(Torres-Vazquez et al., 2001)

ventral denticle belt

(Torres-Vazquez et al., 2001, Marty et al., 2000, Campbell and Tomlinson, 1999)

wing

(Campbell and Tomlinson, 1999)

wing disc | somatic clone

(Zirin and Mann, 2004)

Detailed Description

Statement

Reference

Homozygous clones in the distal wing hinge tend to grow larger than their wild-type twin spots.

(Zirin and Mann, 2004)

The dorsal ectoderm is expanded in mutant embryos and there is a corresponding loss of ventral epidermis (seen as a reduction in the width of the denticle belts). Filzkorper differentiate normally. The first row of anterior facing denticles in segment A1 are typically missing.

(Torres-Vazquez et al., 2001)

Homozygous embryos have reduced ventral denticle bands.

(Marty et al., 2000)

Mutant embryos have narrowed denticle belts. Mutant clones in the distal half and the central region of the A-P axis in adult wing have no phenoytpe. Clones in the anterior and posterior extremes of proximal regions can produce large outgrowths. This phenoytpe is cell autonomous.

(Campbell and Tomlinson, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Suppressed by

Statement

Reference

brk^XH has dorsal ectoderm phenotype, suppressible by shn⁰⁴⁷³⁸

(Torres-Vazquez et al., 2001)

brk^XH has ventral ectoderm phenotype, suppressible by tkv⁸

(Torres-Vazquez et al., 2001)

brk^XH has abdominal 1 ventral denticle belt phenotype, suppressible by shn⁰⁴⁷³⁸

(Torres-Vazquez et al., 2001)

Suppressor of

Statement

Reference

brk^XH is a suppressor of embryo | dorsal phenotype of shn⁰⁴⁷³⁸

(Torres-Vazquez et al., 2001)

brk^XH is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of tkv⁸

(Torres-Vazquez et al., 2001)

brk^XH is a suppressor of embryonic/first instar larval cuticle phenotype of shn³

(Marty et al., 2000)

NOT Suppressor of

Statement

Reference

brk^XH is a non-suppressor of filzkorper phenotype of shn⁰⁴⁷³⁸

(Torres-Vazquez et al., 2001)

Other

Statement

Reference

Mad^B1/Mad¹², brk^XH has anterior-posterior compartment boundary of the wing disc | somatic clone phenotype

(Shen and Dahmann, 2005)

brk^XH, tkv⁸ has filzkorper phenotype

(Torres-Vazquez et al., 2001)

Additional Comments

Genetic Interactions

Statement

Reference

brk^XH/brk^XH, Mad^B1/Mad¹² double mutant wing disc clones have normal apical-basal cell length.

(Widmann and Dahmann, 2009)

brk^XH Mad^B1/Mad¹² clones generated along the A/P boundary of the wing imaginal disc, using the FLP/FRT technique, show no defects in segregation behaviour. As with wild-type clones, these mutant clones stay in the compartment in which they have been generated.

(Shen and Dahmann, 2005)

The elimination of Ts(1Lt;2Lt)sc^S2/+ clones in wild-type wing discs is suppressed if the wing discs are also mutant for brk^XH, although normal proliferation rate is not restored.

(Moreno et al., 2002)

brk^XH ; shn⁰⁴⁷³⁸ double mutant embryos have an intermediate phenotype between the two single mutant phenotypes; the dorsal hole typical of shn mutants is rescued, but the dorsal ectoderm is significantly reduced compared to brk mutant embryos and the cuticle is relatively more ventralised. The Filzkorper are reduced, as in shn⁰⁴⁷³⁸ single mutant embryos. The first row of anterior facing denticles in segment A1 (missing in brk^XH embryos) are recovered. The tkv⁸ "dorsal open" cuticle phenotype is rescued in brk^XH ; tkv⁸ double mutant embryos. These double mutant larvae differentiate a contiguous dorsal epidermis, but the Filzkorper are reduced and are more ventralised than brk^XH single mutant embryos.

(Torres-Vazquez et al., 2001)

shn³ brk^XH double mutant embryos are dorsally closed and differentiate dorsal hairs, but have reduced ventral denticle bands. shn³ brk^XH double mutant clones in the wing disc grow to a large size, in contrast to shn³ single mutant clones.

(Marty et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

brk^unspecified

(Campbell and Tomlinson, 1999)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

Remobilization by Δ2-3 did not lead to reversion of the lethal phenotype.

(Campbell and Tomlinson, 1999)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

brk^XH

(Widmann and Dahmann, 2009)

brk^xh

(Moreno et al., 2002)

Name Synonyms

Secondary FlyBase IDs

References (7)

Report Sections

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