FlyBase Allele Report: Scer\GAL80[αTub84B.PL]

General Information

Symbol

Scer\GAL80^αTub84B.PL

Species

S. cerevisiae

Name

α-Tubulin at 84B promoter construct of Lee

FlyBase ID

FBal0097120

Feature type

allele

Associated gene

Scer\GAL80

Associated Insertion(s)

Carried in Construct

P{tubP-GAL80}

Also Known As

tubP-Gal80, tub-Gal80, tubGAL80, tub.Gal80, Scer\GAL80^{alphaTub84B.PL}, tubulin-Gal80, Tubα1-G80, tub::Gal80

Transgenic product class

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

Mutagen

in vitro construct

(Lee and Luo, 1999)

Progenitor genotype

Carried in construct

P{tubP-GAL80}

Cytology

Description

Expression of GAL80 is controlled by the αTub84B promoter.

(Lee and Luo, 1999)

Allele components

Component

Use(s)

Regulatory region(s)

αTub84B

Encoded product / tool

GAL80

binary expression system - repressor

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell polarity | conditional | somatic clone, with Raf^UAS.F179, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

abnormal cell polarity | conditional | somatic clone, with Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

abnormal sleep, with Bhal\NaChBac^UAS.EGFP, Scer\GAL4^Tdc2.PC

(Crocker et al., 2010)

fertile

(Lee and Luo, 1999)

hyperplasia | conditional | somatic clone, with Raf^UAS.F179, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

hyperplasia | conditional | somatic clone, with Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

increased cell death | somatic clone, with Scer\GAL4^ptc-559.1, rpr^UAS.cZa

(Bilak et al., 2014)

increased cell death | somatic clone, with Scer\GAL4^Tub.PU, Ubr3^KK100920

(Huang et al., 2014)

lethal, with Scer\GAL4^elav.PLu, hid^UAS.cZa

(Mondal et al., 2007)

lethal | temperature conditional, with Scer\GAL4^R15W.elav, hid^UAS.cZa

(Mondal et al., 2007)

radiation resistant | cell non-autonomous, with E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Bilak et al., 2014)

viable

(Lee and Luo, 1999)

viable, with Scer\GAL4^ptc-559.1, rpr^UAS.cZa

(Bilak et al., 2014)

visible, with Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh and Irvine, 2009)

visible, with Scer\GAL4^GMR.PF, yki^{S168A.UAS.Tag:V5}

(Oh and Irvine, 2009)

Phenotype Manifest In

adult Malpighian tubule | conditional | somatic clone, with Raf^UAS.F179, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

adult Malpighian tubule | conditional | somatic clone, with Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C

(Zeng et al., 2010)

adult Malpighian tubule | conditional | somatic clone, with Scer\GAL4^FRT.Act5C, aPKC^{UAS.Tag:CAAX(Unk)}

(Zeng et al., 2010)

adult Malpighian tubule | conditional | somatic clone, with Scer\GAL4^FRT.Act5C, aPKC^ΔN.UAS

(Zeng et al., 2010)

eye, with Scer\GAL4^GMR.PF, yki^{S111A.S168A.S250A.UAS.Tag:V5}

(Oh and Irvine, 2009)

eye, with Scer\GAL4^GMR.PF, yki^{S168A.UAS.Tag:V5}

(Oh and Irvine, 2009)

eye | temperature conditional, with Scer\GAL4^R15W.elav, hid^UAS.cZa

(Mondal et al., 2007)

wing disc | cell non-autonomous, with E2f1^RNAi.UAS.cJa, Scer\GAL4^ptc-559.1

(Bilak et al., 2014)

wing disc | somatic clone, with Scer\GAL4^ptc-559.1, rpr^UAS.cZa

(Bilak et al., 2014)

Detailed Description

Statement

Reference

Causes no external morphological or behavioral abnormalities.

(Lee and Luo, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has radiation resistant | cell non-autonomous phenotype, suppressible | partially by +/Df(3L)mir-ban^Δ1

(Bilak et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, suppressible by Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, suppressible by Dsor1^VDRC.cUa, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, suppressible by Pka-C1^B3

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, suppressible by mTor^ΔP

(Zeng et al., 2010)

NOT suppressed by

Statement

Reference

Scer\GAL4^Tub.PU, Scer\GAL80^αTub84B.PL, Ubr3^KK100920 has increased cell death | somatic clone phenotype, non-suppressible by Df(3L)H99

(Huang et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, non-suppressible by aPKC^k06403

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, non-suppressible by Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has hyperplasia | conditional | somatic clone phenotype, non-suppressible by Stat92E^j6C8

(Zeng et al., 2010)

Suppressor of

Statement

Reference

Rho1^N19.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Dsor1^VDRC.cUa, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

NOT Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a non-suppressor of hyperplasia | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Phenotype Manifest In

Suppressed by

Statement

Reference

Scer\GAL4^ptc-559.1, Scer\GAL80^αTub84B.PL, hid^UAS.cZa, rpr^UAS.cZa has wing disc | cell non-autonomous phenotype, suppressible | partially by +/Df(3L)mir-ban^Δ1

(Bilak et al., 2014)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, suppressible by Rho1^N19.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, suppressible by Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, suppressible by Dsor1^VDRC.cUa, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, suppressible by Pka-C1^B3

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, suppressible by mTor^ΔP

(Zeng et al., 2010)

NOT suppressed by

Statement

Reference

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, non-suppressible by aPKC^k06403

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, non-suppressible by Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL has adult Malpighian tubule | conditional | somatic clone phenotype, non-suppressible by Stat92E^j6C8

(Zeng et al., 2010)

Suppressor of

Statement

Reference

cpb^UAS.cWa/Scer\GAL80^αTub84B.PL is a suppressor | partially of border follicle cell | somatic clone phenotype of not¹

(Badmos et al., 2021)

Rho1^N19.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Raf^K497M.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Dsor1^VDRC.cUa, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Scer\GAL4^LL54/Scer\GAL80^αTub84B.PL is a suppressor of phenotype of Rac1^L89.UAS

(Lee and Luo, 1999)

NOT Suppressor of

Statement

Reference

Pi3K92E^A2860C.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL is a non-suppressor of adult Malpighian tubule | conditional | somatic clone phenotype of Ras85D^V12.UAS, Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Suppresses the dominant eye phenotype caused by the Rac1^L89.Scer\UAS, Scer\GAL4⁵⁴ combination.

(Lee and Luo, 1999)

Complementation and Rescue Data

Rescues

Scer\GAL80^αTub84B.PL, Cul3^UASp.Venus, Scer\GAL4^αTub84B.PL rescues Cul3^gft2

(Hudson and Cooley, 2010)

Comments

Images (0)

Mutant

Wild-type

Stocks (115)

Bloomington

5132

P{hsFLP}1, P{tubP-GAL80}LL1 w^* P{neoFRT}19A

5140

w^*; P{FRT(w^hs)}G13 P{tubP-GAL80}LL2

5135

y¹ w^*; P{neoFRT}82B P{tubP-GAL80}LL3

5191

y¹ w^*; P{tubP-GAL80}LL9 P{neoFRT}80B

42717

P{tubP-GAL80}LL1 w^* P{ey-FLP.N}2 P{neoFRT}19A

42731

P{Ubx-FLP}1, P{tubP-GAL80}LL1 w^* P{neoFRT}19A

5192

y¹ w^*; P{tubP-GAL80}LL10 P{neoFRT}40A/CyO

Kyoto

108063

P{neoFRT}19A, P{tubP-GAL80}LL1, P{hsFLP}1, w^*

108066

y¹ w^*; P{neoFRT}82B P{tubP-GAL80}LL3

108093

y¹ w^*; P{tubP-GAL80}LL9 P{neoFRT}80B

108071

w^*; P{FRT(w^hs)}G13 P{tubP-GAL80}LL2/CyO

108094

y¹ w^*; P{tubP-GAL80}LL10 P{neoFRT}40A/CyO

More stocks available...

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Scer\GAL80^Tub84B.PL

(Casal et al., 2002)

Scer\GAL80^{alphaTub84B.PL}

(Casal et al., 2006, Lawrence et al., 2004)

Scer\GAL80^αTub84B.PL

Scer\Gal80^{alphaTub84B.PL}

(Bischoff and Cseresnyés, 2009)

tubPGAL80

(McGovern and Seeger, 2003)

Name Synonyms

α-Tubulin at 84B promoter construct of Lee

(Lee and Luo, 1999)

Secondary FlyBase IDs

References (177)

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