FlyBase Allele Report: Dmel\aPKC[ΔN.UAS]

General Information

Symbol

Dmel\aPKC^ΔN.UAS

Species

D. melanogaster

Name

Saccharomyces cerevisiae UAS construct a of Betschinger

FlyBase ID

FBal0144482

Feature type

allele

Associated gene

Dmel\aPKC

Associated Insertion(s)

Carried in Construct

P{UAS-aPKC.ΔN}

Also Known As

UAS-aPKC^ΔN, aPKC^ΔN, UAS-aPKC-ΔN, UAS-DaPKCΔN, aPKC.ΔN, UAS-aPKC.ΔN, UAS aPKC^ΔN, UAS-aPKC^act

Key Links

Transgenic product class

inframe_deletion

(Betschinger et al., 2003)

Mutagen

in vitro construct

Nature of the Allele

Transgenic product class

inframe_deletion

(Betschinger et al., 2003)

Mutagen

in vitro construct

(Betschinger et al., 2003)

Progenitor genotype

aPKC^UAS.cBa

(Betschinger et al., 2003)

Carried in construct

P{UAS-aPKC.ΔN}

Cytology

Description

UASt drives expression of a truncated aPKC consisting of amino acids 180-606.

(Betschinger et al., 2003)

Allele components

Component

Use(s)

Regulatory region(s)

UASt

binary expression system - regulatory region

Encoded product / tool

aPKC

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of cancer

is NOT ameliorated by bsk^K53R.UAS

(Bunker et al., 2015)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy, with Scer\GAL4^wor.PA

(Lee et al., 2006)

abnormal planar polarity, with Scer\GAL4^sev.EP

(Djiane et al., 2005)

lethal - all die before end of larval stage, with Scer\GAL4^{neur-GAL4-A101}

(Roegiers et al., 2005)

neoplasia | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

some die during larval stage, with Scer\GAL4^{neur-GAL4-A101}

(Roegiers et al., 2005)

Phenotype Manifest In

adult Malpighian tubule | conditional | somatic clone, with Scer\GAL4^FRT.Act5C, Scer\GAL80^αTub84B.PL

(Zeng et al., 2010)

eye photoreceptor cell, with Scer\GAL4^sev.EP

(Djiane et al., 2005)

larval brain & neuroblast | supernumerary, with Scer\GAL4^wor.PA

(Lee et al., 2006)

larval brain | posterior & neuroblast | supernumerary, with Scer\GAL4^insc-Mz1407

(Betschinger et al., 2006)

ommatidium, with Scer\GAL4^sev.EP

(Djiane et al., 2005)

tormogen cell | somatic clone | increased number, with Scer\GAL4^{neur-GAL4-A101}

(Roegiers et al., 2005)

wing disc | third instar larval stage, with Scer\GAL4^Bx-MS1096

(Bunker et al., 2015)

Detailed Description

Statement

Reference

The expression of aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 leads to a tumor phenotype in third instar larval wing discs.

(Bunker et al., 2015)

Expression of aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 results in neoplastic transformation in the wing disc.

(Skwarek et al., 2014)

Expression of aPKC^ΔN.Scer\UAS in adult Malpighian tubule clones (using the MARCM system, under the control of Scer\GAL80^αTub84B.PL and Scer\GAL4^{Scer\FRT.Act5C}) blocks renal and nephric stem cell (RNSC) proliferation and differentiation.

(Zeng et al., 2010)

Expression of aPKC^ΔN.Scer\UAS in the neuroblasts, driven by Scer\GAL4¹⁴⁰⁷, results in a mild overgrowth of neuroblasts in the posterior brain cortex.

(Betschinger et al., 2006)

Expression of aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^wor.PA results in a slight increase in the number of neuroblasts in the larval brain.

(Lee et al., 2006)

The ommatidia of aPKC^ΔN.Scer\UAS; Scer\GAL4^sev.EP adults are frequently misrotated, symmetrical or lacking some photoreceptors.

(Djiane et al., 2005)

Clones that express aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^{neur-GAL4-A101} show an increased frequency of adult external sense organs with supernumerary socket cells.

(Roegiers et al., 2005)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^ΔN.UAS has neoplasia | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/bsk^K53R.UAS

(Bunker et al., 2015)

Enhancer of

Statement

Reference

Scer\GAL4^da.G32/aPKC^ΔN.UAS is an enhancer of abnormal planar polarity phenotype of arm^F1α, sgg^M11

(Kaplan et al., 2011)

Scer\GAL4^da.G32/aPKC^ΔN.UAS is an enhancer of abnormal cell polarity phenotype of arm^F1α, sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of abnormal planar polarity phenotype of sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of abnormal cell polarity phenotype of sgg^M11

(Kaplan et al., 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-enhancer of abnormal planar polarity phenotype of arm^F1α

(Kaplan et al., 2011)

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-enhancer of abnormal cell polarity phenotype of arm^F1α

(Kaplan et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a suppressor | partially of increased size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a suppressor | partially of abnormal cell size | somatic clone | oogenesis phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^wor.PA/aPKC^ΔN.UAS is a suppressor of abnormal cell polarity phenotype of Scer\GAL4^wor.PA, l(2)gl^3A.UAS

(Atwood and Prehoda, 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-suppressor of abnormal planar polarity phenotype of arm^F1α

(Kaplan et al., 2011)

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-suppressor of abnormal cell polarity phenotype of arm^F1α

(Kaplan et al., 2011)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

Scer\GAL4^Bx-MS1096, aPKC^ΔN.UAS has wing disc | third instar larval stage phenotype, non-suppressible by Scer\GAL4^Bx-MS1096/bsk^K53R.UAS

(Bunker et al., 2015)

Enhancer of

Statement

Reference

Scer\GAL4^da.G32/aPKC^ΔN.UAS is an enhancer of ventral denticle belt phenotype of arm^F1α, sgg^M11

(Kaplan et al., 2011)

arm^F1α, Scer\GAL4^da.G32, aPKC^ΔN.UAS is an enhancer of ventral denticle belt phenotype of sgg^M11

(Kaplan et al., 2011)

NOT Enhancer of

Statement

Reference

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a non-enhancer of mitochondrion | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-enhancer of ventral denticle belt phenotype of arm^F1α

(Kaplan et al., 2011)

Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a suppressor | partially of follicle cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a suppressor | partially of apical part of cell | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^wor.PA/aPKC^ΔN.UAS is a suppressor of larval neuroblast phenotype of Scer\GAL4^wor.PA, l(2)gl^3A.UAS

(Atwood and Prehoda, 2009)

NOT Suppressor of

Statement

Reference

Scer\GAL4^Tub.PU/aPKC^ΔN.UAS is a non-suppressor of mitochondrion | somatic clone phenotype of Drp1^KG03815

(Uttekar et al., 2024)

Scer\GAL4^sns.PK/aPKC^ΔN.UAS is a non-suppressor of garland cell phenotype of Scer\GAL4^sns.PK, baz^RNAi.UAS

(Heiden et al., 2021)

Scer\GAL4^da.G32/aPKC^ΔN.UAS is a non-suppressor of ventral denticle belt phenotype of arm^F1α

(Kaplan et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The neoplasia phenotype resulting from the expression of aPKC^ΔN.Scer\UAS under the control of Scer\GAL4^Bx-MS1096 is not suppressed by the co-expression of bsk^{K53R.Scer\UAS}.

(Bunker et al., 2015)

Co-expression of aPKC^ΔN.Scer\UAS results in normal localization of cell polarity determinants in larval neuroblasts expressing l(2)gl^3A.Scer\UAS via Scer\GAL4^wor.PA.

(Atwood and Prehoda, 2009)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescues

Scer\GAL4^da.G32/aPKC^ΔN.UAS rescues aPKC^k06403

(Kaplan et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

51673

w^*; P{UAS-aPKC.ΔN}3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

aPKC^ΔN.Scer\UAS

aPKC^ΔN.UAS

aPKC^ΔN

(Lodge et al., 2021, Wirtz-Peitz et al., 2008, Roegiers et al., 2005)

Name Synonyms

Saccharomyces cerevisiae UAS construct a of Betschinger

(Betschinger et al., 2003)

Secondary FlyBase IDs

References (34)

Report Sections

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