FlyBase Allele Report: Dmel\Drp1[KG03815]

General Information

Symbol

Dmel\Drp1^KG03815

Species

D. melanogaster

Name

FlyBase ID

FBal0131515

Feature type

allele

Associated gene

Dmel\Drp1

Associated Insertion(s)

P{SUPor-P}Drp1^KG03815

Carried in Construct

Also Known As

drp1^KG, KG03815

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Gene Disruption Project members, 2001-)

Progenitor genotype

Associated Insertion(s)

P{SUPor-P}Drp1^KG03815

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

2L:2,584,872..2,584,872 [+]

(Uttekar et al., 2024, Swinter et al., 2023, Ponte et al., 2020, Tsakiri et al., 2019, Fernandes and Rao, 2011, Rikhy et al., 2007, Ly, 2005.3.2, Verstreken et al., 2005, Gene Disruption Project members, 2001-)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates Parkinson's disease

modeled by Pink1^B9

(Fernandes and Rao, 2011)

ameliorates amyotrophic lateral sclerosis type 1

modeled by Sod1^G85R

(Nemtsova et al., 2023)

ameliorates Parkinson's disease 1

modeled by Hsap\SNCA^UAS.cFa

(Krzystek et al., 2021)

ameliorates neurodegenerative disease

modeled by Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal - all die before end of P-stage

(Krzystek et al., 2021)

Phenotype Manifest In

autophagic vacuole & larval fat body | somatic clone | cell autonomous

(Arsham and Neufeld, 2009)

embryonic/larval brain | larval stage | somatic clone

(Dubal et al., 2022)

intermediate neuronal progenitor | larval stage | somatic clone

(Dubal et al., 2022)

larval multidendritic neuron

(Nemtsova et al., 2023)

lysosome & larval fat body | somatic clone | cell autonomous

(Arsham and Neufeld, 2009)

mitochondrial derivative | germline clone

(Aldridge et al., 2007)

mitochondrion & primary spermatocyte | germ-line clone

(Aldridge et al., 2007)

mitochondrion | larval stage

(Nemtsova et al., 2023, Krzystek et al., 2021)

mitochondrion | larval stage | somatic clone

(Dubal et al., 2022)

Nebenkern | germline clone

(Aldridge et al., 2007)

synapse | larval stage

(Nemtsova et al., 2023)

type II neuroblast | larval stage | somatic clone

(Dubal et al., 2022)

Detailed Description

Statement

Reference

Drp1^KG03815 type II neuroblast clones in the larval brain exhibit clustered mitochondria.

(Dubal et al., 2022)

Drp1^KG03815 clones in fat bodies from well-fed early third instar larvae accumulate lysosomes, as indicated by elevated LysoTracker staining, and show redistribution of the Atg8a product into autophagosomes.

(Arsham and Neufeld, 2009)

Drp1^KG03815/+ mutants are fully viable.

(Poole et al., 2008)

Primary spermatocytes in homozygous germline clones induced in males contain abnormally clustered mitochondria. 52% of early round spermatids derived from these spermatocytes retain mitochondrial material and in all cases the mitochondria appear not to have separated properly during meiosis, remaining connected and appearing to pass through the cytoplasmic bridges between spermatids. The remaining 48% of the mutant spermatids have little or no mitochondrial material. At later stages of flagellar elongation, the mutant spermatids have defects in unfurling and elongation of mitochondria, with the spermatids containing spaghetti-like interconnected masses of mitochondria that fail to elongate properly and are associated with multiple nuclei. The ratio of the observed mitochondrial masses to nuclei is 8:34 , consistent with each mass typically representing the mitochondrial material from all four meiotic products of a single spermatocyte.

(Aldridge et al., 2007)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Drp1^KG03815/Drp1[+], Pink1^B9 has partially lethal - majority die phenotype

(Fernandes and Rao, 2011, Poole et al., 2008)

Drp1^KG03815/Drp1[+], park²⁵ has lethal phenotype

(Poole et al., 2008)

Drp1^KG03815/Drp1[+], Pink1^B9 has abnormal developmental rate phenotype

(Poole et al., 2008)

Drp1^KG03815/Drp1[+], Pink1^B9 has short lived phenotype

(Poole et al., 2008)

Drp1^KG03815/Drp1[+], Pink1^B9 has decreased body size phenotype

(Poole et al., 2008)

Phenotype Manifest In

Suppressed by

Statement

Reference

Drp1^KG03815 has mitochondrion | larval stage phenotype, suppressible by Sod1^G85R/Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815 has larval multidendritic neuron phenotype, suppressible by Sod1^G85R/Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815 has synapse | larval stage phenotype, suppressible by Sod1^G85R/Sod1^G85R

(Nemtsova et al., 2023)

Suppressor of

Statement

Reference

Drp1^KG03815 is a suppressor of mitochondrion | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815 is a suppressor of larval multidendritic neuron phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815 is a suppressor of synapse | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815 is a suppressor of neuronal cell body | larval stage phenotype of Sod1^G85R

(Nemtsova et al., 2023)

Drp1^KG03815/Drp1[+] is a suppressor of mitochondrion | larval stage phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Drp1^KG03815/Drp1[+] is a suppressor of larval segmental nerve phenotype of Scer\GAL4^Appl.G1a, Zzzz\CAG^{127Q.UAS.Tag:HA}

(Swinter et al., 2023)

Drp1^KG03815 is a suppressor of mitochondrion | larval stage phenotype of Hsap\SNCA^UAS.cFa, Scer\GAL4^Appl.G1a

(Krzystek et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

Drp1^KG03815/+ in combination with park²⁵/park²⁵ mutants results in lethality. Viability of flies Drp1^KG03815/+ is unaffected in a park²⁵/+ background.

Drp1^KG03815/+ in combination with Pink1^B9 hemizygous mutations results in almost complete lethality. The few surviving adults are developmentally delayed and are substantially smaller and short lived than single Pink1^B9 hemizygous mutants. Viability of Drp1^KG03815/+ flies is unaffected in a heterozygous Pink1^B9/+ background.

(Poole et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Drp1¹

(Ly, 2005.3.2)

Drp1²

(Ly, 2005.3.2)

Drp1¹

(Verstreken et al., 2005)