FlyBase Allele Report: Dmel\Sod1[G85R]

General Information

Symbol

Dmel\Sod1^G85R

Species

D. melanogaster

Name

FlyBase ID

FBal0343865

Feature type

allele

Associated gene

Dmel\Sod1

Associated Insertion(s)

TI{TI}Sod1^G85R

Carried in Construct

Also Known As

dSod1^G85R

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

ends-out gene targeting

(Şahin et al., 2017)

I-CreI

(Şahin et al., 2017)

FLPase

(Şahin et al., 2017)

I-SceI

(Şahin et al., 2017)

Progenitor genotype

Associated Insertion(s)

TI{TI}Sod1^G85R

Cytology

Description

Amino acid replacement: G?R.

(Şahin et al., 2017)

The equivalent mutation to the ALS-associated G85R mutation in Hsap\SOD1 has replaced the endogenous gene at the Sod1 locus. (Note names are based on the Hsap\SOD1 amino acid numbering system.) A LoxP "scar" of 72 nt remains. (Note that this allele also contains a natural A1013C polymorphism, which leads to the N98K missense mutation, according to the human amino acid numbering system.)

(Şahin et al., 2017)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:11,112,665..11,112,665 [-]

Nucleotide change:

G11112665C

Reported nucleotide change:

G?C

Amino acid change:

G84R | Sod1-PA

Comment:

Analogous mutation in human SOD1 implicated in ALS; mutation introduced into fly gene by homologous recombination; site of nucleotide substitution in fly gene specified by author. G85 in human SOD1 aligns with G84 in Drosophila ortholog Sod1.

(Şahin et al., 2017)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of amyotrophic lateral sclerosis type 1

CEA

(Nemtsova et al., 2023, Agudelo et al., 2020, Held et al., 2019, Şahin et al., 2017)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

model of amyotrophic lateral sclerosis type 1

is ameliorated by COX6AL2^HMC03530

(Nemtsova et al., 2023)

is ameliorated by Drp1^KG03815

(Nemtsova et al., 2023)

is ameliorated by ND-51L1^HMS02423

(Nemtsova et al., 2023)

is ameliorated by SdhBL^HMJ22015

(Nemtsova et al., 2023)

is ameliorated by gbb^UAS.cKa

(Held et al., 2019)

is ameliorated by sax^CA.UAS.cUa

(Held et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

This allele represents a human variant implicated in disease.

(Şahin et al., 2017)

SOD1:p.Gly86Arg

(FlyBase curators, 2019-)

Variants Synonym(s)

SOD1:p.Gly85Arg

Associated human disease model(s)

amyotrophic lateral sclerosis 1

External database links

ClinVar: SOD1_14758

Comments concerning this variant

Phenotypic Data

Phenotypic Class

abnormal size | larval stage

(Nemtsova et al., 2023)

Phenotype Manifest In

Detailed Description

Statement

Reference

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

Marf^B, Sod1^G85R has lethal phenotype

(Nemtsova et al., 2023)

Phenotype Manifest In

NOT Enhanced by

Statement

Reference

Sod1^G85R has larval multidendritic neuron phenotype, non-enhanceable by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, non-enhanceable by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, non-enhanceable by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

Suppressed by

Statement

Reference

Sod1^G85R has mitochondrion | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/Miro^UAS.cGa

(Nemtsova et al., 2023)

Sod1^G85R has larval multidendritic neuron phenotype, suppressible by Scer\GAL4^109(2)80/Miro^UAS.cGa

(Nemtsova et al., 2023)

Sod1^G85R has axon | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/Miro^UAS.cGa

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, suppressible by Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R has larval multidendritic neuron phenotype, suppressible by Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, suppressible by Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R has neuronal cell body | larval stage phenotype, suppressible by Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/ND-51L1^HMS02423

(Nemtsova et al., 2023)

Sod1^G85R has larval multidendritic neuron phenotype, suppressible by Scer\GAL4^109(2)80/ND-51L1^HMS02423

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/SdhBL^HMJ22015

(Nemtsova et al., 2023)

Sod1^G85R has larval multidendritic neuron phenotype, suppressible by Scer\GAL4^109(2)80/SdhBL^HMJ22015

(Nemtsova et al., 2023)

Sod1^G85R has larval multidendritic neuron phenotype, suppressible by Scer\GAL4^109(2)80/COX6AL2^HMC03530

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/COX6AL2^HMC03530

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/Miro^UAS.cGa

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/ND-51L1^HMS02423

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/SdhBL^HMJ22015

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, suppressible by Scer\GAL4^109(2)80/COX6AL2^HMC03530

(Nemtsova et al., 2023)

NOT suppressed by

Statement

Reference

Sod1^G85R has larval multidendritic neuron phenotype, non-suppressible by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

Sod1^G85R has mitochondrion | larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

Sod1^G85R has synapse | larval stage phenotype, non-suppressible by Scer\GAL4^109(2)80/Coq8^HMS04484

(Nemtsova et al., 2023)

NOT Enhancer of

Statement

Reference

Sod1^G85R is a non-enhancer of larval multidendritic neuron phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of mitochondrion | larval stage phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of mitochondrion | larval stage phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of larval multidendritic neuron phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of mitochondrion | larval stage phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of larval multidendritic neuron phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of axon | larval stage phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of mitochondrion | larval stage phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of larval multidendritic neuron phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of axon | larval stage phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of mitochondrion | larval stage phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of larval multidendritic neuron phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of axon | larval stage phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of axon | larval stage phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-enhancer of axon | larval stage phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Suppressor of

Statement

Reference

Sod1^G85R/Sod1^G85R is a suppressor of mitochondrion | larval stage phenotype of Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R/Sod1^G85R is a suppressor of larval multidendritic neuron phenotype of Drp1^KG03815

(Nemtsova et al., 2023)

Sod1^G85R/Sod1^G85R is a suppressor of synapse | larval stage phenotype of Drp1^KG03815

(Nemtsova et al., 2023)

NOT Suppressor of

Statement

Reference

Sod1^G85R is a non-suppressor of larval multidendritic neuron phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of mitochondrion | larval stage phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of larval multidendritic neuron phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of axon | larval stage phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of mitochondrion | larval stage phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of larval multidendritic neuron phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of axon | larval stage phenotype of Dhc64C^4-19

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of mitochondrion | larval stage phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of larval multidendritic neuron phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of axon | larval stage phenotype of Dhc64C^HMS01587, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of axon | larval stage phenotype of Khc²⁷

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of axon | larval stage phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of mitochondrion | larval stage phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of larval multidendritic neuron phenotype of sw^Dic-1

(Nemtsova et al., 2023)

Sod1^G85R is a non-suppressor of mitochondrion | larval stage phenotype of Miro^UAS.cGa, Scer\GAL4^109(2)80

(Nemtsova et al., 2023)

Other

Statement

Reference

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has axon | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has synapse | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has mitochondrion | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has neuronal cell body | larval stage phenotype

(Nemtsova et al., 2023)

Miro^UAS.cGa, Scer\GAL4^109(2)80, Sod1^G85R has larval multidendritic neuron phenotype

(Nemtsova et al., 2023)

Additional Comments

Genetic Interactions

Statement

Reference

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Sod1^G85R

dSod1^G85R-hr

(Nemtsova et al., 2023)

dSod1^G85R

(Nemtsova et al., 2023, Held et al., 2019)

dsod1^G85R

(Agudelo et al., 2020)

dsod^G85R

(Şahin et al., 2017)

Name Synonyms

Secondary FlyBase IDs

References (5)

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