FB2024_03 , released June 25, 2024
Allele: Dmel\GluRIIAM614R.UAS.Tag:MYC
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General Information
Symbol
Dmel\GluRIIAM614R.UAS.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0097264
Feature type
allele
Associated gene
Dmel\GluRIIA
Associated Insertion(s)
Carried in Construct
P{UAS-GluRIIA.M614R}
Also Known As
UAS-GluRIIAM/R, UAS-GluRIIAM614R
Key Links
Transgenic product class
dominant_negative_variant
(DiAntonio et al., 1999)
missense_variant
(DiAntonio et al., 1999)
Mutagen
Nature of the Allele
Transgenic product class
dominant_negative_variant
(DiAntonio et al., 1999)
missense_variant
(DiAntonio et al., 1999)
Mutagen
in vitro construct
(DiAntonio et al., 1999)
Progenitor genotype
Carried in construct
P{UAS-GluRIIA.M614R}
Cytology
Description

UASt regulatory sequences drive expression of a dominant negative form of GluRIIA (carries the amino acid replacement M614R). The protein is tagged with Tag:MYC.

(DiAntonio et al., 1999)
Allele components
Component
Use(s)
Regulatory region(s)
UASt
Encoded product / tool
GluRIIA
Tagged with
Tag:MYC
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Expression of GluRIIAM614R.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mhc.PW in larval neuromuscular junctions results in decreased amplitude of miniature excitatory junction currents (mEJCs) as compared to controls, EJCs are maintained at wild type levels, and quantal content is increased.

      (Penney et al., 2016)

      In neuromuscular junctions of GluRIIAM614R.Scer\UAS.T:Hsap\MYC; Scer\GAL4C142 larvae there is a decrease in quantal size, but an increase in quantal content. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae).

      (Haghighi et al., 2003)

      Expression of two copies of GluRIIAM614R.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4how-24B results in lethality. Expression of one copy of GluRIIAM614R.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4how-24B results in viable adults which do not have any obvious behavioural abnormalities. There is a significant reduction in the quantal size at the neuromuscular junction.

      (DiAntonio et al., 1999)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Lrrke03680/+ blocks the increased quantal content seen in larval NMJs expressing GluRIIAM614R.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4Mhc.PW, via a decrease in excitatory junction current (EJC) amplitude.

      (Penney et al., 2016)

      The increase in quantal content in neuromuscular junctions of GluRIIAM614R.Scer\UAS.T:Hsap\MYC; Scer\GAL4C142 larvae is partially suppressed by CaMKIIT287D.Scer\UAS : There is an decrease of 25% (p < 0.01) (55% decrease when corrected for nonlinear summation) in quantal content compared to GluRIIAM614R.Scer\UAS.T:Hsap\MYC; Scer\GAL4C142 larvae. This decrease is due to a significant reduction (p < 0.01) in the amplitude of excitatory postsynaptic potentials (EPSPs). There is no effect on mini-EPSPs. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). witHA4/witHA5 suppresses the increase in quantal content seen in the neuromuscular junctions of GluRIIAM614R.Scer\UAS.T:Hsap\MYC; Scer\GAL4C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). witHA4/witHA5 suppresses the increase in the excitatory postsynaptic potential amplitude seen in the neuromuscular junctions of Rat\CaMKIINĪ±I.Scer\UAS.cHa; Scer\GAL4C142 larvae. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae). The excitatory postsynaptic potential (EPSP) and mini-EPSP amplitudes are significantly decreased in witHA4/witHA5 larvae by the presence of GluRIIAM614R.Scer\UAS.T:Hsap\MYC with Scer\GAL4C142. (Data from electrophysiological recordings made from muscle 6 in segment A3 of wandering 3rd instar larvae).

      (Haghighi et al., 2003)

      The addition off GluRIIAM614R.Scer\UAS.T:Hsap\MYC to hiw mutants leads to a an 18% decrease in quantal size, no change in the size of the evoked response, and a compensatory 30% increase in quantal content.

      (DiAntonio et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      Reported As
      Symbol Synonym
      Glu-RIIAM614R.Scer\UAS.T:Hsap\MYC
      GluRIIAM614R.Scer\UAS.T:Hsap\MYC
      GluRIIAM614R.UAS.Tag:MYC
      Name Synonyms
      Secondary FlyBase IDs
        References (8)