FlyBase Allele Report: Dmel\Cdc27[S092309]

General Information

Symbol

Dmel\Cdc27^S092309

Species

D. melanogaster

Name

FlyBase ID

FBal0100461

Feature type

allele

Associated gene

Dmel\Cdc27

Associated Insertion(s)

P{lacW}Cdc27^S092309

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

P-element activity

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Deak et al., 2003)

Mutagen

P-element activity

(Deak, 1999.9.15)

Progenitor genotype

Associated Insertion(s)

P{lacW}Cdc27^S092309

Cytology

Description

Insertion within the 5'-untranslated region 253bp upstream of the ATG initiator codon.

(Deak et al., 2003)

The insertion is in the 5'UTR of the Cdc27 transcript.

(Deak, 1999.9.15)

Allele components

Component

Use(s)

Inserted element

P{lacW}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

transposable_element_insertion_site

3L:7,248,934..7,248,934

(Deak et al., 2003, Deak, 1999.9.15)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

chromatin

(Deak et al., 2003)

chromosome

(Deak et al., 2003)

condensed chromosome & neuroblast & larva

(Deak et al., 2003)

mitosis & nuclear chromosome

(Deak and Glover, 1999)

mitotic cell cycle

(Deak and Glover, 1999)

mitotic metaphase

(Deak et al., 2003)

spindle & neuroblast & larva

(Deak et al., 2003)

Detailed Description

Statement

Reference

In Cdc27^S092309 mutants, there is a high proportion of cells in a metaphase-like state (a ratio of 8.2 metaphase:anaphase compared to 2.8 in wild-type). Along with a low proportion of cells in anaphase. This suggests that Cdc27^S092309 cells arrest or delay in metaphase.

Cdc27^S092309 anaphase cells are frequently disorganised, with chromosomes lagging along the spindle. A low frequency (~3% of mitoses) of polyploid cells are also found in Cdc27^S092309 cells.

In Cdc27^S092309 larval stage brains the sister chromosomes of at least one chromosome are found to be separated in 20% of all mitotic figures. 19% of Cdc27^S092309 mitotic cells are found to contain a 4N complement of chromosomes in which there are four sites of hybridization indicating that the sister chromosomes are separated. This is compared to a frequency of 0.4% in wild-type metaphase cells.

In mitotic Cdc27^S092309 cells, the centromeric regions of the two pairs of major autosomes are present as a series of four separate pairs of dots, indicative of chromosome separation. Approximately 3.0% of Cdc27^S092309 cells contain a replicated tetraploid set of chromosomes.

Chromatin in Cdc27^S092309 mitotic cells is distributed in a broad band. Cdc27^S092309 cells exhibit overcondensed chromosomes.

Using BubR1 to stain the spindle integrity checkpoint complex, up to 16 separated kinetochores of sister chromosomes are observed in Cdc27^S092309 cells, similar to the phenotype observed in polo hypomorphs.

In Cdc27^S092309 neuroblasts, CycA is maintained at high levels in all cells arrested in a metaphase-like state. CycB accumulates to high levels in all metaphase arrested Cdc27^S092309 cells.

(Deak et al., 2003)

Mutation results in a high mitotic index, overcondensed chromosomes, lagging or bridged chromosomes in anaphase and telophase as well as polyploidy in homozygous larval neuroblasts.

(Deak and Glover, 1999)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

Cdc27^S092309 has abnormal mitotic cell cycle phenotype, enhanceable by polo^S025604

(Deak et al., 2003)

Cdc27^S092309 has lethal phenotype, enhanceable by polo¹

(Deak et al., 2003)

Cdc27^S092309 has abnormal mitotic cell cycle phenotype, enhanceable by polo¹

(Deak et al., 2003)

Enhancer of

Statement

Reference

Cdc27^S092309 is an enhancer of abnormal mitotic cell cycle phenotype of polo^S025604

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of lethal phenotype of polo^S025604

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of lethal phenotype of polo¹

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of abnormal mitotic cell cycle phenotype of polo¹

(Deak et al., 2003)

Suppressor of

Statement

Reference

Cdc27^S092309 is a suppressor of abnormal mitotic cell cycle phenotype of tws^S061805

(Deak et al., 2003)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdc27^S092309 has mitotic anaphase phenotype, enhanceable by tws^S061805

(Deak et al., 2003)

Cdc27^S092309 has mitotic telophase phenotype, enhanceable by tws^S061805

(Deak et al., 2003)

Cdc27^S092309 has aster & neuroblast phenotype, enhanceable by polo^S025604

(Deak et al., 2003)

Cdc27^S092309 has spindle & neuroblast phenotype, enhanceable by polo^S025604

(Deak et al., 2003)

Cdc27^S092309 has aster & neuroblast phenotype, enhanceable by polo¹

(Deak et al., 2003)

Cdc27^S092309 has spindle & neuroblast phenotype, enhanceable by polo¹

(Deak et al., 2003)

Enhancer of

Statement

Reference

Cdc27^S092309 is an enhancer of aster & neuroblast phenotype of polo^S025604

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of spindle & neuroblast phenotype of polo^S025604

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of aster & neuroblast phenotype of polo¹

(Deak et al., 2003)

Cdc27^S092309 is an enhancer of spindle & neuroblast phenotype of polo¹

(Deak et al., 2003)

Suppressor of

Statement

Reference

Cdc27^S092309 is a suppressor of mitotic anaphase phenotype of tws^S061805

(Deak et al., 2003)

Cdc27^S092309 is a suppressor of mitotic telophase phenotype of tws^S061805

(Deak et al., 2003)

Additional Comments

Genetic Interactions

Statement

Reference

The lethal phase of Cdc27^S092309 is advanced to an earlier developmental stage when in combination with polo¹. The stage of death is also earlier than with polo¹ alone. In terms of the mitotic index, the metaphase:anaphase ratio, the condensation state of the chromosomes and the levels of mitotic cyclins, the phenotype of Cdc27^S092309 polo¹ cells do not differ significantly from that of the Cdc27^S092309 single mutant. The polo¹ phenotype with respect to spindle pole organisation is enhanced by the Cdc27^S092309 mutation. Mitotically arrested Cdc27^S092309 polo¹ cells, lack the MPM2 epitope at their spindle poles, consistent with diminished levels of polo kinase activity at the spindle poles. Cdc27^S092309 is epistatic to polo¹ in respect of mitotic progression and cyclin degradation. Cdc27^S092309 polo¹ double mutant cells display overcondensed chromosomes. In Cdc27^S092309 polo¹ double mutants the mitotic index, proportions of metaphase:anaphase cells and the proportion of polyploids are similar to that in the Cdc27^S092309 mutant alone. The lethal phase of Cdc27^S092309 is advanced to an earlier developmental stage when in combination with polo^S025604. The stage of death is also earlier than with polo^S025604 alone. There is no apparent shift in the lethal phase in the Cdc27^S092309 tws^S061805 double mutant. Cytological analysis of neuroblasts show an elevated mitotic index and a dramatic reduction of metaphase figures replaced by an elevated frequency of anaphase-telophase figures equivalent to the tws^S061805 mutant alone. Therefore the Cdc27^S092309 tws^S061805 double mutant overcomes the metaphase arrest seen in the Cdc27^S092309 single mutant. From the analysis of CycB localisation, it is postulated that Cdc27^S092309 tws^S061805 double mutant mitotic cells are capable of entering anaphase without activation of the APC/C. Anaphase in these cells is highly abnormal.

(Deak et al., 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

Cdc27^S092309 is rescued by Cdc27^+t6.2

(Deak et al., 2003)

Not rescued by

Cdc27^S092309 is not rescued by Cdc27^ΔBam.t6.2

(Deak et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Cdc27^S092309

Cdc27^mks

cdc27^makos

(Slack et al., 2007)

l(3)092309

mks

(Deak and Glover, 1999)

mks¹

(Deak et al., 2003, Deak, 1999.9.15)

Name Synonyms

Secondary FlyBase IDs

References (4)

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